Research Journal of Pharmacy and Technology

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Preparation and Physical Characterization of Andrographolide-Chitosan-Alginate Microparticles prepared by Ionic Gelation–Freeze Drying


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1 Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
     

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This study aimed to determine the effect of alginate concentration on physical characterization (morphology, physical state, and encapsulation efficiency) of andrographolide-chitosan-alginate microparticles prepared by ionic gelation- freeze drying method. Andrographolide-chitosan-alginate microparticles were produced with various amount of alginate by ionic gelation method using tripholyphospate (TPP) as crosslinker and maltodextrin as a lyoprotectant. The physical characteristics were evaluated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FT-IR), Differential Thermal Analyzer (DTA), Powder X-ray diffractrometer (PXRD), drug content was evaluated using High Pressure Liquid Chromatography (HPLC). The results showed that andrographolide crystallinity changed after encapsulated inside the microparticles supported by changes in IR spectra, and melting point. Furthermore, increased alginate concentration showed rougher surface morphology. The amount of alginate affected the characteristics of the andrographolide-chitosan-alginate microparticles by altering the crystallinity and lowering melting point. The highest encapsulation efficiency shown by microparticles with 0.5% alginate.

Keywords

Microparticles, Andrographolide, Chitosan, Alginates, Ionic Gelation.
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  • Preparation and Physical Characterization of Andrographolide-Chitosan-Alginate Microparticles prepared by Ionic Gelation–Freeze Drying

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Authors

Retno Sari
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Sandra Tunggadewi
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Abhimata Paramanandana
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Helmy Yusuf
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia

Abstract


This study aimed to determine the effect of alginate concentration on physical characterization (morphology, physical state, and encapsulation efficiency) of andrographolide-chitosan-alginate microparticles prepared by ionic gelation- freeze drying method. Andrographolide-chitosan-alginate microparticles were produced with various amount of alginate by ionic gelation method using tripholyphospate (TPP) as crosslinker and maltodextrin as a lyoprotectant. The physical characteristics were evaluated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FT-IR), Differential Thermal Analyzer (DTA), Powder X-ray diffractrometer (PXRD), drug content was evaluated using High Pressure Liquid Chromatography (HPLC). The results showed that andrographolide crystallinity changed after encapsulated inside the microparticles supported by changes in IR spectra, and melting point. Furthermore, increased alginate concentration showed rougher surface morphology. The amount of alginate affected the characteristics of the andrographolide-chitosan-alginate microparticles by altering the crystallinity and lowering melting point. The highest encapsulation efficiency shown by microparticles with 0.5% alginate.

Keywords


Microparticles, Andrographolide, Chitosan, Alginates, Ionic Gelation.

References