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Assessment of Antioxidant and Antiangiogenic Activities of Ethanolic Root extract of Cassia occidentalis
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Context: The process by which new blood vessels grow is termed as Angiogenesis. In the adult, except for a few physiological processes such as menses, wound healing, and placental formation, all angiogenic processes are pathologic. Angiogenic imbalance in which proangiogenic factors predominate over antiangiogenic factors is tumor angiogenesis. Inhibition of blood vessel growth, is a way to prevent tumour growth and other angiogenesis-dependent diseases is antiangiogenesis. Aim: The present study aims to determine the antioxidant and antiangiogenic activities of ethanolic ischolar_main extract of Cassia occidentalis. Materials and methods: Antioxidant activity was evaluated against various free radicals like Nitric oxide scavenging, Hydroxyl radicals and Phosphomolybdenum assay using Ascorbic acid as standard. The most common study used to evaluate the antiangiogenic activity is chick embryo chorioallantoic membrane (CAM) , an in vivo assay. Results: Ethanolic ischolar_main extract of Cassia occidentalis exhibited a significant antioxidant status which is evident from their IC50 values 21.42 ug/mL in Nitric oxide scavenging method, 22.3 ug/mL in hydroxyl radical. In phosphomolybdenum assay, there is concentration-dependent increase in absorbance. The results obtained are similar to that of standard drug ascorbic acid. In anti-oxidant activity, percentage inhibition was determined by calculating IC50 values . By decreasing the capillary networks proliferation in a dose (50 to 150 μg/egg) dependent manner which is probably related to the inhibition of neovascularization. The present study results indicated significant antiangiogenic effect on chorioallantoic membrane. Conclusion: The plant extract has shown good free radical scavenging activity indicative from their percentage inhibition. It also exhibits significant antiangiogenic activity, and this is a possible rationale for its folkloric use as an anticancer agent.
Keywords
Antioxidant Activity, Antiangiogenic Activity, Chick Chorioallantoic Membrane Assay, Neovascularization and Cassia occidentalis.
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- Giordano FJ, Johnson RS. Angiogenesis: the role of the microenvironment in flipping the switch. Current Opin Genet Dev. 2001; 11(1):35-40.
- Udagawa T, Fernandez A, Achilles EG, Folkman J, D’Amato RJ. Persistence of microscopic human cancers in mice: alterations in the angiogenic balance accompanies loss of tumor dormancy. The FASEB J. 2002; 16 (11):1361-70.
- Carmeliet P. Angiogenesis in health and disease. Nature Med. 2003;9(6):653.
- Brown RA, Weiss JB. Neovascularisation and its role in the osteoarthritic process. Ann Rheumatic Dis. 1988;47 (11):881.
- Waltman D, Gitter K, Yannuzzi L, Schatz H. Choroidal neovascularization associated with choroidal nevi. Am J Ophthalmol. 1978; 85: 704-10.
- Folkman J. Anti-angiogenesis: new concept for therapy of solid tumors. Ann Surg. 1972; 175(3):409.
- Folkman J, Kalluri R. Cancer without disease. Nature. 2004; 427(6977):787.
- Moses M, Langer R. Inhibitors of angiogenesis. Biotech. 1991; 9:630-34.
- Rao MP. Ashwagandha (Withania somniifera)- ayurvedic bequest for the patients of cancer: an update on current research. Int J Res Ayurveda Pharma. 2010; 1(2):234-38.
- Khandelwal KR. Practical Pharmacognosy (11th ed.), Nirali Prakashan, Pune. 2004; pp. 149–156.
- Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol., 1999; 299:152-178.
- Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 2002; 10 (3).
- Fazel S, Hamidreza M, Rouhollah G, Verdian-rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Soc. Adv. Hort. 2010; 12(1):69-70.
- Smirnoff N, Cumbes QJ. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry. 1989; 28:1057-60.
- Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex. Specific application to the determination of vitamin E. Analy Biochem 1999;269:337-41.
- L. Marcocci, J.J. Maguire, M.T. Droy-Lafaix, L. Packer.The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761 Biochem Biophys Res Commun, 201 (1994), pp. 748-755
- Noor A. Lokman , Alison S. F. Elder, Carmela Ricciardelli and Martin K. Oehler , Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian Cancer Invasion and Metastasis. Int. J. Mol. Sci. 2012; 13, 9959-9970.
- Martínez‐Poveda B, Quesada AR, Medina MÁ. Hyperforin, a bio‐active compound of St. John's Wort, is a new inhibitor of angiogenesis targeting several key steps of the process. Int J Cancer. 2005; 117(5):775-80.
- Ribatti D, Ribatti D. Chapter 1. History of research on tumor angiogenesis. 2009:1-7.
- Folkman J, Klagsbrun M. Angiogenic factors. Science. 1987; 235(4787):442-7.
- Folkman J. Anti-angiogenesis: new concept for therapy of solid tumors. Ann Surg. 1972;175(3):409.
- Folkman J, Kalluri R. Cancer without disease. Nature. 2004;427(6977):787.
- Moses M, Langer R. Inhibitors of angiogenesis. Biotech. 1991; 9:630-34.
- Kalimuthu K, Prabakaran R, Saraswathy M. Antiangiogenic activity of Boucerosia diffusa and Boucerosia truncatocoronata extracts in chick Chorioallantoic Membrane (CAM). Int J Cur Microbiol Applied Sci. 2014; 3 (8):107-14.
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