Research Journal of Pharmacy and Technology

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Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations


Affiliations
1 Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
2 Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad, India
3 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034., India
     

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Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Sofosbuvir in pharmaceutical dosage form. The Method A is based on reaction of Sofosbuvir with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Sofosbuvir with ferric chloride and 2,2'- bipyridyl to form a red colored ferroin complex measured at 520 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm and 520 nm respectively and obey the Beer’s law in the concentration range of 10-140 μg/mL and. 5-50 μg /mL respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Sofosbuvir in bulk drug and in its formulations.

Keywords

UV-Visible Spectrophotometry, Sofosbuvir, Ferric Chloride, 1, 10-Phenanthroline, and 2, 2’ Bipyridyl.
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  • The American Society of Health-System Pharmacists. Archived from the original on 2016-12-01. Retrieved Nov 30, 2016.

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  • Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations

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Authors

Nakka Raju
Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
P. Raveendra Reddy
Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
P. Janaki Pathi
Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad, India
N. Appala Raju
Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034., India

Abstract


Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Sofosbuvir in pharmaceutical dosage form. The Method A is based on reaction of Sofosbuvir with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Sofosbuvir with ferric chloride and 2,2'- bipyridyl to form a red colored ferroin complex measured at 520 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm and 520 nm respectively and obey the Beer’s law in the concentration range of 10-140 μg/mL and. 5-50 μg /mL respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Sofosbuvir in bulk drug and in its formulations.

Keywords


UV-Visible Spectrophotometry, Sofosbuvir, Ferric Chloride, 1, 10-Phenanthroline, and 2, 2’ Bipyridyl.

References