Research Journal of Pharmacy and Technology

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Lab–Built Semi-Automated Stop–Flow System for Spectrophotometric Phosphate Determination in Different Water Samples


Affiliations
1 Marine Chemistry Department, Marine Science Center, University of Basra, Iraq
2 Chemistry Department, Education College for Pure Sciences, University of Basra, Iraq
     

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A lab built semi-automated stopped-flow system was employed for spectrophotometric determination of phosphate in different water samples. The construction system consists made software programs. The first one was UNO type used to control and manipulates a home-made injection-pump stepper-motor type to control the reaction-time in the flow cell. The other one was Mega type and which was used as data-logger to manipulate and recording the results as stopped-flow peaks by using Microsoft Excel 2010 program. The linearity was up to 2 μg/ml with regression coefficient of 0.9960. The detection limit was 0.05 μg/ml and the samples can be analyzed at rate exceeding 45 samples/h.

Keywords

Stopped-Flow, Arduino, Uno, Mega, Phosphate, Injection Pump.
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  • Lab–Built Semi-Automated Stop–Flow System for Spectrophotometric Phosphate Determination in Different Water Samples

Abstract Views: 248  |  PDF Views: 0

Authors

Mohammad Th. K. Al-Balaawi
Marine Chemistry Department, Marine Science Center, University of Basra, Iraq
K. H. Al-Sowdani
Chemistry Department, Education College for Pure Sciences, University of Basra, Iraq

Abstract


A lab built semi-automated stopped-flow system was employed for spectrophotometric determination of phosphate in different water samples. The construction system consists made software programs. The first one was UNO type used to control and manipulates a home-made injection-pump stepper-motor type to control the reaction-time in the flow cell. The other one was Mega type and which was used as data-logger to manipulate and recording the results as stopped-flow peaks by using Microsoft Excel 2010 program. The linearity was up to 2 μg/ml with regression coefficient of 0.9960. The detection limit was 0.05 μg/ml and the samples can be analyzed at rate exceeding 45 samples/h.

Keywords


Stopped-Flow, Arduino, Uno, Mega, Phosphate, Injection Pump.

References