Research Journal of Pharmacy and Technology

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In Silico Analysis of the Betuline from the Fiddler Crab, Uca annulipes and its Antimicrobial as Well as Anti Lung Cancer Activities.


Affiliations
1 Universiti Sultan ZainalAbidin, Terengganu, Malaysia
2 Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
3 Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
     

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Introduction: <i>In silico</i> analysis of the bioactive compound betuline from the fiddler crab, Uca annulipes and its antimicrobial activities were studied. The fiddler crabs, Uca annulipes were collected from Muttukadu Estuary located on the Kanchipuram District, Tamil Nadu, India, 35 K.M away from Chennai, on the East Coast Road route to Kovalam. Materials and Methods: Fiddler crabs were collected by hand picking method. From the muscle mass of the Crab the bioactive compound was isolated by GC-MS analysis. Molecular docking was performed to identify the protein ligand responsible for the affinity with lung cancer causing tumour cells. Results and Discussion: Hence betuline is the potential lead molecule for the inhibition of lung cancer protein and the most important residues for potential drug target as carbon hydrogen bond, conventional hydrogen bond and Vander Waals interaction. In vitro studies of antimicrobial activities clearly indicated that the different concentrations of betuline bioactive compound have the potential to control the bacteria such as Escherichia coli and Pseudomonas aeruginosa. In vitro studies of anticancer activities also evidently showed the different concentrations of bioactive compound betuline have the potential to control the proliferation of lung cancer cells. Conclusion: Reports are very scanty on this species studied and the reports are very old and hence this present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs against the lung cancer. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the lung cancer.

Keywords

Betuline, Escherichia coli, Lung Cancer, Pseudomonas aeruginosa and Uca annulipes.
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  • Hartnoll, R.G., 1975.The Grapsidae and Ocypodidae (Decapoda: Brachyura) of Tanzania. J. Zool., 177: 305–328.

  • Icely, J.D., and Jones, D.A. 1975. Factors affecting the distribution of the genus Uca (Crustacea: Ocypodidade) on an East African shore. Estuarine and Coastal Marine Science., 6: 315–325.

  • Dahdouh-Guebas, F., M. Giuggioli, A. Oluoch, M. Vannini and Cannicci, S. 1999. Feeding habits of non-ocypodid crab from two mangrove forests in Kenya. Bulletin of Marine Science. 64: 291–297.

  • Nobbs, M., and McGuiness, K.A. 1999. Developing methods for quantifying the apparent abundance of fiddler crabs, (Ocypodidade: Uca) in mangrove habitats. Australian Journal of Ecology., 24: 43–49.

  • Macia, A., Quincardete, I. and Paula, J. 2001.A comparison of alternative methods for estimating population density of the fiddler crab Uca annulipes at Saco mangrove. Inhaca Island (Mozambique). Hydrobiologia., 449: 213–219.

  • Skov, M.W., and Hartnoll, R.G. 2001. Comparative suitability of binocular observation, burrow counting and excavation for the quantification of the mangrove fiddler crab, Uca annulipes (Milne Edwards, H.). Hydrobiologia; 449: 201–212.

  • Skov, M.W., Vannini, M. Shunnula, J.P. Hartnoll, R.G. and Cannicci, S. 2002. Quantifying the density of mangrove crabs. Ocypodidae and Grapsidae. Mar. Biol., 141: 725–732.

  • Aggressive Waves in the Lemon-Clawed Fiddler Crab (Uca perplexa): A Regional “Dialect” in Fiji, Judith S. Weis and Peddrick Weis. ISRN Biodiversity. Research Article (4 pages), Article ID 319590, Volume 2013 (2013)

  • Backwell, P.Y., Christy, J. H. Telford, S. R. Jennions, M. D and Passmore, N.I. 2000. Dis honest signalling in a fiddler crab. Proceedings of the Royal Society Series, B 267: 1–6.

  • Crane, J., 1975. Eastern pacific Expeditions of the New York Zoological society. XXVI. Crabs of the genus Uca from the West coast of Central America. Zoological NY, 26:145-208.

  • Macintosh, D.J., 1988. The ecology and physiology of decapods of mangrove swamps. Symp. Zool. Soc. Lond. 59: 315-341.

  • Ashton, E.C., McIntonsh D.J. and Hoghart, P.J. 2003. A baseline studyof the diversity and community ecology of crab and molluscan macrofauna in the Sematan mangrove forest, Sarawak, Malaysia. Journal of Tropical Ecology, 19:127–142.

  • Hofmann, H.S., Bartling, B. and Simm, A. et al., 2006. Identification and classification of differentially expressed genes in non-small cell lung cancer by expression profiling on a global human 59.620-element oligonucleotide array. 16(3): 587-595.

  • Ellis, P.M., 2012. The importance of multidisciplinary team management of patients with non-small-cell lung cancer. Current oncology (Toronto, Ont.). (1): 7-15.

  • Analysis of the Clinicopathological Characteristics and Risk Factors in Patients with Lung Cancer and Chronic Obstructive Pulmonary Disease, Jian-Long Miao, Jing-Jing Cai, Xiao-Feng Qin, and Rui-Juan Liu. BioMed Research International. Research Article (5 pages), Article ID 8398156, Volume 2018 (2018)

  • Effect of Comorbidity on Lung Cancer Diagnosis Timing and Mortality: A Nationwide Population-Based Cohort Study in Taiwan, Shinechimeg Dima, Kun-Huang Chen, Kung-Jeng Wang, Kung-Min Wang, and Nai-Chia Teng. BioMed Research International. Research Article (9 pages), Article ID 1252897, Volume 2018 (2018)

  • American Lung Association, 2014c. Lung Cancer Fact Sheet. Available at: http://www.lung.org/lung-disease/lung-cancer/resources/facts.

  • Identifying the Best Marker Combination in CEA, CA125, CY211, NSE, and SCC for Lung Cancer Screening by Combining ROC Curve and Logistic Regression Analyses: Is It Feasible?, Qixian Yang, Ping Zhang, Rongqiang Wu, Kefeng Lu, and Hongxing Zhou. Disease Markers. Research Article (12 pages), Article ID 2082840, Volume 2018 (2018)

  • Association between VEGF Gene Polymorphisms and the Susceptibility to Lung Cancer: An Updated Meta-Analysis, Fengming Yang, Zhiqiang Qin, Chuchu Shao, Weitao Liu, Ling Ma, Yongqian Shu, and Hua Shen. BioMed Research International. Review Article (16 pages), Article ID 9271215, Volume 2018 (2018)

  • Ma. J., Ward E.M. and Smith, R. et al., 2013. Annual number of lung cancer deaths potentially avertable by screening in the United States. 1; 119 (7): 1381-1385.

  • Clinical Lung Cancer Genome Project (CLCGP) 2013. Network Genomic Medicine (NGM). A Genomics-Based Classification of Human Lung Tumors.

  • Heuvers, M.E., Hegmans, J.P. and Stricker, B.H. et al., 2012. Improving lung cancer survival; time to move on BMC pulmonary medicine.12: 77.

  • Wouters, B.G., Wang, L.H. and Brown, J.M. 1999. Tirapazamine: a new drug producing tumor specific enhancement of platinum-based chemotherapy in non-small-cell lung cancer. Annals of oncology: Official Journal of the European Society for Medical Oncology/ESMO. 10 (5): 29-33.

  • Nascimento, A.V., Bousbaa, H. and Ferreira, D. et al., 2014. Non-small Cell Lung Carcinoma: An Overview on Targeted Therapy. Current Drug Targets.

  • Canadian Cancer Society. Cancer Information page. Syndrome of Inappropriate Antidiuretic Hormone. Available at: http://www.cancer.ca/en/cancer-information/diagnosis-and-treatment/managing-side-effects/syndrome-of-inappropriate-antidiuretic-hormone/?region=qc. Copyright © 2014. Accessed 2/25/2014.

  • Yoh, K., Kubota, K., and Nomura, M. et al., 2003. Cushing's syndrome associated with adenocarcinoma of the lung. Internal medicine (Tokyo, Japan). 42 (9): 831-833.

  • Brennan, P., Hainaut, P. and Boffetta, P. 2011. Genetics of lung-cancer susceptibility. The lancet oncology. 12 (4): 399-408.

  • CDC. Centers for Disease Control and Prevention. Press Release. About one in five U.S. adult cigarette smokers have tried an electronic cigarette. Available at: http://www.cdc.gov/media/releases/2013/p0228_electronic_cigarettes.html. Last updated 2/28/2013a. Accessed 2/21/2014.

  • Kligerman, S., and White, C., 2011. Epidemiology of lung cancer in women: risk factors, survival, and screening. American journal of roentgenology. 196 (2):287-295.

  • Cohen, A.J. and Pope, C.A. 1995. 3rd. Lung cancer and air pollution. Environmental health perspectives. 103 (8): 219-224.

  • Davidson, M.R., Gazdar, A.F. and Clarke, B.E. 2013. The pivotal role of pathology in the management of lung cancer. Journal of thoracic disease. 5 (5): 463-S478.


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  • In Silico Analysis of the Betuline from the Fiddler Crab, Uca annulipes and its Antimicrobial as Well as Anti Lung Cancer Activities.

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Authors

Thant Zin
Universiti Sultan ZainalAbidin, Terengganu, Malaysia
J. Sivakumar
Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
C. Shanmuga Sundaram
Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, India
U. S. Mahadeva Rao
Universiti Sultan ZainalAbidin, Terengganu, Malaysia

Abstract


Introduction: <i>In silico</i> analysis of the bioactive compound betuline from the fiddler crab, Uca annulipes and its antimicrobial activities were studied. The fiddler crabs, Uca annulipes were collected from Muttukadu Estuary located on the Kanchipuram District, Tamil Nadu, India, 35 K.M away from Chennai, on the East Coast Road route to Kovalam. Materials and Methods: Fiddler crabs were collected by hand picking method. From the muscle mass of the Crab the bioactive compound was isolated by GC-MS analysis. Molecular docking was performed to identify the protein ligand responsible for the affinity with lung cancer causing tumour cells. Results and Discussion: Hence betuline is the potential lead molecule for the inhibition of lung cancer protein and the most important residues for potential drug target as carbon hydrogen bond, conventional hydrogen bond and Vander Waals interaction. In vitro studies of antimicrobial activities clearly indicated that the different concentrations of betuline bioactive compound have the potential to control the bacteria such as Escherichia coli and Pseudomonas aeruginosa. In vitro studies of anticancer activities also evidently showed the different concentrations of bioactive compound betuline have the potential to control the proliferation of lung cancer cells. Conclusion: Reports are very scanty on this species studied and the reports are very old and hence this present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs against the lung cancer. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the lung cancer.

Keywords


Betuline, Escherichia coli, Lung Cancer, Pseudomonas aeruginosa and Uca annulipes.

References