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In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7


Affiliations
1 Departement of Medical Chemistry, University of Indonesia, Indonesia
2 University of Indonesia, Indonesia
3 Indonesian Medical Education and Research Institute, University of Indonesia, Indonesia
     

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Breast cancer most commonly occurs in women worldwide, in 2012 there were 1.670.000 cases with the second most prevalence in the world (25% of the total incidence of cancer). Drugs used so far have not been effective and many resistance occurs, it is necessary to a discovery of herbs that contain polyphenols as anticancer. Kaempferia pandurata Roxb plant has been identified to contain various essential oils and several flavonoid compounds that have demonstrated many biological activities. This research was done by simulation study with molecular docking approach which then extraction, the result of the extract was tested in vitro activity against Breast Cancer Cell Line MCF-7. The docking results show that the flavonoid compound against the target ERbreast cancer has an enthalpy energy ΔG -11.75 kcal/mol, the phytochemical results support that the flavonoids compound dominates the composition of the Kaempferia pandurata Roxb. The Result of in vivo test with MTT method IC50 value of standard cisplatin, hexane extract was 8.42; 26.70 μg/ml respectively to MCF-7 cell line showed that Kaempferia pandurata Roxb extract very potential as an antibreast cancer.

Keywords

Molecular Docking, Phytochemical Test, Antibreast Cancer, Kaempferia pandurata Roxb., MCF-7 Cell Line.
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  • In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7

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Authors

Fadilah
Departement of Medical Chemistry, University of Indonesia, Indonesia
Lowilius Wiyono
University of Indonesia, Indonesia
Brenda Cristie Edina
University of Indonesia, Indonesia
Risya Amelia Rahmawati
University of Indonesia, Indonesia
Linda Erlina
Departement of Medical Chemistry, University of Indonesia, Indonesia
Aryo Tedjo
Departement of Medical Chemistry, University of Indonesia, Indonesia
Rafika Indah Paramita
Indonesian Medical Education and Research Institute, University of Indonesia, Indonesia

Abstract


Breast cancer most commonly occurs in women worldwide, in 2012 there were 1.670.000 cases with the second most prevalence in the world (25% of the total incidence of cancer). Drugs used so far have not been effective and many resistance occurs, it is necessary to a discovery of herbs that contain polyphenols as anticancer. Kaempferia pandurata Roxb plant has been identified to contain various essential oils and several flavonoid compounds that have demonstrated many biological activities. This research was done by simulation study with molecular docking approach which then extraction, the result of the extract was tested in vitro activity against Breast Cancer Cell Line MCF-7. The docking results show that the flavonoid compound against the target ERbreast cancer has an enthalpy energy ΔG -11.75 kcal/mol, the phytochemical results support that the flavonoids compound dominates the composition of the Kaempferia pandurata Roxb. The Result of in vivo test with MTT method IC50 value of standard cisplatin, hexane extract was 8.42; 26.70 μg/ml respectively to MCF-7 cell line showed that Kaempferia pandurata Roxb extract very potential as an antibreast cancer.

Keywords


Molecular Docking, Phytochemical Test, Antibreast Cancer, Kaempferia pandurata Roxb., MCF-7 Cell Line.

References