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New RP-UPLC method for The Simultaneous Determination of Haloperidol and Benzhexol in Tablets


Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, India
     

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Haloperidol is an anti-psychotic drug and Benzhexol (Trihexyphenidyl) is an anticholinergic drug. A newstability indicating UPLC method has been developed for the simultaneous determination of Haloperidol and Benzhexol and the method was validated. Waters UPLC with Empower software, with Acquity UPLC SB C18 x 1.7μ. column and PDA detector was used for the simultaneous determination of Haloperidol and Benzhexol. A mixture of phosphate buffer and Acetonitrile taken in the ratio 56:44 v/v was used as mobile phase with a flow rate of 0.2 ml/min (UV detection at 245 nm) (Injection volume 1μl). Haloperidol and Benzhexol have shown linearregression equations, y=13361x+476.2(R² = 0.9998) and y =10609 x + 899.5 (R² = 0.9998). The LOD and LOQ are found to be 0.03 μg/mL and 0.10 μg/mL for Haloperidol and 0.03μg/mL and 0.09 μg/mL for Benzhexolrespectively. The present liquid chromatographic method is precise, accurate and can be used for the simultaneous estimation of Haloperidol and Benzhexol tablets.

Keywords

Haloperidol, Benzhexol (Trihexyphenidyl), RP-UPLC, Isocratic Mode, Validation.
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  • British Pharmacopoeia, London, The British Pharmacopoeia Commission. 1; 2008:1047.

  • Indian Pharmacopoeia, New Delhi, The Controller of Publications, Govt of India. 1; 1996: 91.

  • Bognar IT, Altes U, Beinhauer C, Kessler I, Fuder H. A muscarinic receptor different from the M1, M2, M3 and M4 subtypes mediates the contraction of the rabbit iris sphincter. Naunyn Schmiedebergs Arch Pharmacol. 345(6); 1992: 611-618.

  • Dorje F, Wess J, Lambrecht G, Tacke R, Mutschler E, Brann MR: Antagonist binding profiles of five cloned human muscarinic receptor subtypes. J Pharmacol Exp Ther. 256(2); 1991: 727-733.

  • Srikantha D and Ramesh Raju R.A RP-HPLC method for simultaneous determination of Haloperidol and Trihexyphenidyl hydrochloride in tablet dosage form. Asian Journal of Pharmaceutical and Clinical Research. 7; Suppl 7; 2014: 14-18.

  • Amulya E, Naveen Kumar N, Mounika CH, Kowmudi V, Supriya N, Ramya Madhuri K. Development and validation of RP-HPLC method for the simultaneous estimation of Haloperidol and Trihexyphenidyl in API and combined tablet dosage form. International Journal of Applied Pharmaceutical Sciences and Research. 3 (3); 2018: 36-40.

  • Wate SP, Borkar AA. RP-HPLC estimation of Haloperidol and Trihexyphenidyl HCl in tablets. International Journal of Chem Tech Res. 1(3); 2009: 675-676.

  • Wate SP, Borkar AA. Simultaneous spectrophotometric estimation of Haloperidol and Trihexyphenidyl HCL in tablets. Indian Journal of Pharmaceutical Sciences. 72(2); 2010:265-267.

  • Manoj Charde, Imran Sheikh, Sarika Dhole and Avinash. V. Kasture. Simultaneous Estimation of Benzhexol hydrochloride and Haloperidol by absorbance correction method. Journal of Pharmacy Research. 3(9); 2010: 2099-2101.

  • ICH Validation of analytical procedures: Text and methodology Q2 (R1), International Conference on Harmonization, 2005.


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  • New RP-UPLC method for The Simultaneous Determination of Haloperidol and Benzhexol in Tablets

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Authors

Rajesh Pudi
Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, India
Mukthinuthalapati Mathrusri Annapurna
Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, India

Abstract


Haloperidol is an anti-psychotic drug and Benzhexol (Trihexyphenidyl) is an anticholinergic drug. A newstability indicating UPLC method has been developed for the simultaneous determination of Haloperidol and Benzhexol and the method was validated. Waters UPLC with Empower software, with Acquity UPLC SB C18 x 1.7μ. column and PDA detector was used for the simultaneous determination of Haloperidol and Benzhexol. A mixture of phosphate buffer and Acetonitrile taken in the ratio 56:44 v/v was used as mobile phase with a flow rate of 0.2 ml/min (UV detection at 245 nm) (Injection volume 1μl). Haloperidol and Benzhexol have shown linearregression equations, y=13361x+476.2(R² = 0.9998) and y =10609 x + 899.5 (R² = 0.9998). The LOD and LOQ are found to be 0.03 μg/mL and 0.10 μg/mL for Haloperidol and 0.03μg/mL and 0.09 μg/mL for Benzhexolrespectively. The present liquid chromatographic method is precise, accurate and can be used for the simultaneous estimation of Haloperidol and Benzhexol tablets.

Keywords


Haloperidol, Benzhexol (Trihexyphenidyl), RP-UPLC, Isocratic Mode, Validation.

References