Development and Validation for Estimation of Abiraterone acetate in Bulk and Pharmaceutical Dosage Form by UPLC

A simple, Robust, stability indicated method was developed for the estimation of Abiraterone by RP-UPLC technique. Chromatographic conditions used are, stationary phase SB C8 100 x 3.0 mm, 1.8. Mobile phase 0.01N NaH₂PO₄: Acetonitrile in the ratio of 60:40v/v and flow rate were maintained at 0.3 ml/min, detection wave length was 235 nm, and column temperature was set to 30^oC. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R² value was found to be as 0.999. Precision was found to be 0.6 for repeatability and 1.1 for intermediate precision. LOD and LOQ are 0.09μg/ml and 0.27μg/ml respectively. Degradation studies of Abiraterone were done, in all conditions and it is within the acceptable range.