Research Journal of Pharmacy and Technology

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High Performance Liquid Chromatographic Method for the Determination of Mesalamine in Bulk Drug and Pharmaceutical Dosage Forms


Affiliations
1 Department of Chemistry, R.V.R. & J.C. College of Engineering, Guntur, A.P.,, India
2 Department of Chemistry, S.V. University, Tirupathi, A.P.,, India
     

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A simple, rapid and sensitive reverse phase high performance liquid chromatographic method has been developed for the determination of Mesalamine in bulk drug and pharmaceutical dosage forms. It was carried out using a mobile phase of methanol and 0.025 M potassium dihydrogen phosphate buffer (65:35 v/v) on a kromosil C18 column (250 X 4.6 mm, 5μm) in an isocratic mode at a flow rate of 1.0 ml/min. with detection at 250 nm using a UV detector. The linearity was obtained in the concentration range of 20 –100 μg/ml. The developed method was found to be accurate for the determination of Mesalamine in tablet dosage forms.

Keywords

Mesalamine, RP-HPLC, Method development, Validation, Recovery experiments.
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  • High Performance Liquid Chromatographic Method for the Determination of Mesalamine in Bulk Drug and Pharmaceutical Dosage Forms

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Authors

Srinivasa Rao Narala
Department of Chemistry, R.V.R. & J.C. College of Engineering, Guntur, A.P.,, India
K. Saraswathi
Department of Chemistry, S.V. University, Tirupathi, A.P.,, India

Abstract


A simple, rapid and sensitive reverse phase high performance liquid chromatographic method has been developed for the determination of Mesalamine in bulk drug and pharmaceutical dosage forms. It was carried out using a mobile phase of methanol and 0.025 M potassium dihydrogen phosphate buffer (65:35 v/v) on a kromosil C18 column (250 X 4.6 mm, 5μm) in an isocratic mode at a flow rate of 1.0 ml/min. with detection at 250 nm using a UV detector. The linearity was obtained in the concentration range of 20 –100 μg/ml. The developed method was found to be accurate for the determination of Mesalamine in tablet dosage forms.

Keywords


Mesalamine, RP-HPLC, Method development, Validation, Recovery experiments.

References