Research Journal of Pharmacy and Technology

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Uv-visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin


Affiliations
1 MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
2 MM School of Pharmacy, MM University, Sadopur, Ambala, Haryana,, India
3 Maharishi Markandeshwar University, Solan, HP,, India
     

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Background: Curcumin shows degradation in the solution of high pH. There is a need for development of a method which can estimate the drug release precisely and accurately. The UV spectroscopy due to its simplicity and specificity is the most popular for method development for the determination of drugs. Objective: The present study was aimed to develop the UV-Visible spectrophotometric method for the assessment of curcumin and tetrahydrocurcumin (THC) using a stable solvent system. Method: The solubility of curcumin and tetrahydrocurcumin was determined at various physiological pH levels. Non-ionic surfactant, tween 80 was used with selected media to avoid the degradation of drugs. The selected solvent system was further validated according to guidelines of the international conference on harmonization (ICH) and the analytical parameters like linearity, veracity, sensitivity and accuracy were studied. Results: The solvent systems Phosphate buffer of pH 6.8+2% tween 80 and Phosphate buffer of pH 6.8+1% tween 80 were found optimum for estimation of curcumin and THC respectively. All the validation parameters were found within the range for developed methods. Conclusion: The proposed method is very simple and can be used for routine quality control testing of curcumin and tetrahydrocurcumin.

Keywords

Curcumin, Tetrahydrocurcumin, Phosphate buffer pH 6.8, Tween 80, UV-Visible spectrophotometric method development.
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  • Uv-visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin

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Authors

Jai Bharti Sharma
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
Shailendra Bhatt
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
Vipin Saini
MM School of Pharmacy, MM University, Sadopur, Ambala, Haryana,, India
Rupesh K. Gautam
Maharishi Markandeshwar University, Solan, HP,, India
Manish Kumar
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India

Abstract


Background: Curcumin shows degradation in the solution of high pH. There is a need for development of a method which can estimate the drug release precisely and accurately. The UV spectroscopy due to its simplicity and specificity is the most popular for method development for the determination of drugs. Objective: The present study was aimed to develop the UV-Visible spectrophotometric method for the assessment of curcumin and tetrahydrocurcumin (THC) using a stable solvent system. Method: The solubility of curcumin and tetrahydrocurcumin was determined at various physiological pH levels. Non-ionic surfactant, tween 80 was used with selected media to avoid the degradation of drugs. The selected solvent system was further validated according to guidelines of the international conference on harmonization (ICH) and the analytical parameters like linearity, veracity, sensitivity and accuracy were studied. Results: The solvent systems Phosphate buffer of pH 6.8+2% tween 80 and Phosphate buffer of pH 6.8+1% tween 80 were found optimum for estimation of curcumin and THC respectively. All the validation parameters were found within the range for developed methods. Conclusion: The proposed method is very simple and can be used for routine quality control testing of curcumin and tetrahydrocurcumin.

Keywords


Curcumin, Tetrahydrocurcumin, Phosphate buffer pH 6.8, Tween 80, UV-Visible spectrophotometric method development.

References