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In Vitro Assessment of Sun Protection Factor (Spf) and Antioxidant Activity of Viola Odorata Extracts


Affiliations
1 Master Student in Pharmaceutical Chemistry and Drug Control Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syrian Arab Republic
2 Doctor in Pharmacognosy Department, Faculty of Pharmacy, Tishreen University, Lattakia,, Syrian Arab Republic
3 Doctor in Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syrian Arab Republic
     

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The harmful effects of sunlight on the skin have triggered several studies. They concentrated on the synthesis or extraction of natural compounds that can absorb ultraviolet (UV) radiation. In this study, we investigated the in vitro photo-protective potential of Viola odorata flowers extracts, in three states (fresh, dried, frozen), using three solvents (chloroform, ethyl acetate, ethanol) by sequential extraction. In vitro SPF values were determined by spectrophotometric method. Antioxidant activity was tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Total phenolic content (TPC) was measured using Folin-Ciocalteu method. The ethanolic extract fraction, at the concentration of 2 mg/ml, from the three flowers states had the best SPF values (27.249, 26.978, 27.907) respectively, followed by ethyl acetate extract fraction of dried flowers (SPF=21.11). The highest DPPH inhibition (about 85%) was also obtained in ethanolic extract fraction from the three states of flowers. The ethanolic extract fraction of fresh flowers showed the highest total phenolic content (18.575 mg GAE/g crud extract), followed by ethanolic extract fraction of dried flowers (15.06 mg GAE/g crud extract). There was a significant positive correlation between SPF values and each of antioxidant activity (R=0.92) and TPC (R=0.748) in the different extract fractions. Our results showed that ethnolic Viola odorata flowers extract, in different states, has a great potential to be used as a sunscreen agent due to its polyphenol content, which is also associated with strong antioxidant activity.

Keywords

SPF, Antioxidant, Polyphenols, Viola odorata, Extract.
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  • Neelima G, et al., Formulation and Evaluation of Sun Protection Factor of Poly Herbal Sunscreen Cream. Research Journal of Topical and Cosmetic Sciences. 2016 June 7(1): 09-14. Doi: 10.5958/2321-5844.2016.00002.9

  • Greinert, R., et al., European Code against Cancer 4th Edition: Ultraviolet radiation and cancer. The International Journal of Cancer Epidemiology,Detection, and Prevention. 2015 Dec 395: S75-S83. Doi: 10.1016/j.canep.2014.12.014

  • Baldisserotto, A., et al., Moringa oleifera leaf extracts as multifunctional ingredients for “natural and organic” sunscreens and photoprotective preparations. Molecules, 2018 Mar 23(3): 664-680. Doi: 10.3390/molecules23030664

  • Saha, D, et al., Skin cancer: dance of death. Asian Journal of Pharmceutical Research. 2011 June 1(2): 34-36. Doi: 10.5958/2231–5691

  • Panich, U., et al., Ultraviolet radiation-induced skin aging: the role of DNA damage and oxidative stress in epidermal stem cell damage mediated skin aging. Stem Cells International, 2016 Apr 2016: doi: 7370642

  • Patil, S., et al., Formulation of gel and its UV protective study of some medicinal flowers. Research Journal of Topical and Cosmetic Science. 2011 Jul-Dec. 2(2): 64-65

  • Nash, J.F., Human safety and efficacy of ultraviolet filters and sunscreen products. Dermatol Clin, 2006 Jan. 24(1): 35-51. Doi: 10.1016/j.det.2005.09.006

  • Siller, A., et al., Update about the effects of the sunscreen ingredients oxybenzone and octinoxate on humans and the environment. Plastic Surgical Nursing Journal, 2018 Oct-Dec. 38(4): 157-160. Doi: 10.1097

  • Russo, J., et al., Photoallergic Contact Dermatitis to Sunscreens Containing Oxybenzone in La Plata, Argentina. Actas Dermo-Sifiliográficas (English Edition), 2018 Jul-Aug. 109(6): 521-528. Doi: 10.1016/j.adengel.2018.05

  • Ratnasooriya, W., et al., In vitro sunscreen activity of Methanolic root extract of a Sri Lankan grass Heteropogon contortus. Asian Journal of Pharmaceutical Analysis, 2018 Apr-Jun. 8(2): 65-68. Doi: 10.5958/2231-5675.2018.00012.1

  • Maipas, S. and P. Nicolopoulou-Stamati, Sun lotion chemicals as endocrine disruptors. Hormones, 2015 Apr. 14(1): 32-46. Doi: 10.14310/horm.2002.1572

  • Schmutzler, C., et al., The ultraviolet filter benzophenone 2 interferes with the thyroid hormone axis in rats and is a potent in vitro inhibitor of human recombinant thyroid peroxidase. Endocrinology, 2007 June. 148(6): 2835-2844. Doi: 10.1210/en.2006-1280

  • Roy, A. and R.K. Sahu, Formulation and Development of Herbal Sunscreen Cream. Research Journal of Topical and Cosmetic Sciences, 2014 Jan-June. 5(1): 12-14.

  • Cefali, L.C., et al., Flavonoid-Enriched Plant-Extract-Loaded Emulsion: A Novel Phytocosmetic Sunscreen Formulation with Antioxidant Properties. Antioxidants, 2019 Oct. 8(10): 443. doi: 10.3390/antiox8100443

  • Dudonné, S., et al., Phenolic composition and antioxidant properties of poplar bud (Populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging. Journal of Agricultural and Food Chemistry, 2011 Mar. 59(9): 4527-4536. Doi: 10.1021/jf104791t

  • Xue, Y.-L., et al., Isolation and tyrosinase inhibitory effects of polyphenols from the leaves of persimmon, Diospyros kaki. Journal of Agricultural and Food Chemistry, 2011 May. 59(11): 6011-6017. Doi: 10.1021/jf200940h

  • Rugină, D., et al., Antiproliferative and apoptotic potential of cyanidin-based anthocyanins on melanoma cells. International Journal of Molecular Sciences, 2017 Apr. 18(5): 949. Doi: 10.3390/ijms18050949

  • Mittal, P., et al., Phytochemical and pharmacological potential of viola odorata. International Journal of Pharmacognosy (IJP), 2015 May.2(5): 215-220. Doi: 10.13040/IJPSR.0975-8232.IJP.2(5).215-20

  • Tiwari, P. and R.K. Patel, Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods. Asian Journal of Research in Chemistry, 2013 Mar. 6(3): 204-208.

  • Brand-Williams, W., M.-E. Cuvelier, and C. Berset, Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 1995 June. 28(1): 25-30.

  • Lokapure, S.G., et al., In vitro Evaluation of Sun Protection factor and Diffusion study of Hibiscus rosa-sinensis L. flower Extract Gel. Research Journal of Pharmacy and Technology, 2014 Jun. 7(6): 643-647

  • Gonçalves, A.F.K., et al., Anti-oxidant capacity, total phenolic contents and HPLC determination of rutin in Viola tricolor (L) flowers. Free Radicals and Antioxidants, 2012 Oct-Dec. 2(4): 32-37. Doi: 10.5530/ax.2012.4.6

  • Omsone, L., Z. Kruma, and R. Galoburda, Comparison of different solvents and extraction methods for isolation of phenolic compounds from horseradish roots (Armoracia rusticana). International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 2012 . (6)4: 236-241.

  • Boussetta, N., et al., Valorization of oilseed residues: Extraction of polyphenols from flaxseed hulls by pulsed electric fields. Industrial Crops and Products, 2014 Jan. 52: 347-353. Doi: 10.1016/j.indcrop.2013.10.048

  • Durling, N.E., et al., Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures. Food chemistry, 2007. 101(4): 1417-1424. Doi: 10.1016/j.foodchem.2006.03.050

  • Santarelli, V., et al., Response of organic and conventional apples to freezing and freezing pre-treatments: Focus on polyphenols content and antioxidant activity. Food chemistry, 2020 Mar. 308(125570) . Doi: 10.1016/j.foodchem.2019.125570

  • Ghasemzadeh, A., H.Z. Jaafar, and A. Rahmat, Changes in antioxidant and antibacterial activities as well as phytochemical constituents associated with ginger storage and polyphenol oxidase activity. BMC Complementary and Alternative Medicine, 2016 Sep. 16, 382(2016). Doi: 10.1186/s12906-016-1352-1

  • Narayanan, D.L., R.N. Saladi, and J.L. Fox, Ultraviolet radiation and skin cancer. International Journal of Dermatology, 2010 Sep. 49(9): 978-986. Doi: 10.1111/j.1365-4632.2010.04474.x

  • Khelker, T., N. Haque, and A. Agrawal, Ultraviolet Protection potential of Curcuma longa L. and Citrus sinensis (L.) Osbeck. Research Journal of Pharmacy and Technology, 2017 Dec. 10(12): 4282-4284. Doi: 10.5958/0974-360X.2017.00784.3

  • Rangasamy, P., et al., Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. Asian Journal of Pharmaceutical Analysis, 2019 Apr- Jun. 9(2):67-79. Doi: 10.5958/2231-5675.2019.00014.0

  • Liu, J., et al., The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chemistry, 2011 May. 126(1): 261-269.

  • Khan, R.A., et al., Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chemistry Central Journal, 2012 Feb. 6(1): doi: 10.1186/1752-153X-6-12

  • Cefali, L., et al., Plant‐based active photoprotectants for sunscreens. International Journal of Cosmetic Science, 2016 Aug. 38(4): 346-353. Doi: 10.1111/ics.12316

  • Hupel, M., N. Poupart, and E.A. Gall, Development of a new in vitro method to evaluate the photoprotective sunscreen activity of plant extracts against high UV-B radiation. Talanta, 2011 Sep. 86: 362-371. Doi: 10.1016/j.talanta.2011.09.029

  • Nunes, A.R., et al., Photoprotective potential of medicinal plants from Cerrado biome (Brazil) in relation to phenolic content and antioxidant activity. Journal of Photochemistry and Photobiology B: Biology, 2018 Dec. 189: 119-123. Doi: 10.1016/j.jphotobiol.2018.10.013

  • Khazaeli, P. and M. Mehrabani, Screening of sun protective activity of the ethyl acetate extracts of some medicinal plants. Iranian Journal of Pharmaceutical Research, 2010 7(1): 5-9. Doi: 10.22037/ijpr.2010.738

  • Singh, M. and V. Sharma, Spectrophotometric determination of Sun Protection Factor and antioxidant potential of an herbal mixture. Biotechnology Journal International, 2015 10(3): 1-8. Doi: 10.9734/BBJ/2016/21434

  • Philip F. BuldersHerbal Medicine. IntechOpen. UK. 2017

  • Cockell, C.S. and J. Knowland, Ultraviolet radiation screening compounds. Biological Reviews, 1999. 74(3): 311-345. Doi: 10.1111/j.1469-185X.1999.tb00189.x

  • Radice, M., et al., Herbal extracts, lichens and biomolecules as natural photo-protection alternatives to synthetic UV filters. A systematic review. Fitoterapia, 2016. Oct. 114: 144-162. Doi: 0.1016/j.fitote.2016.09.003

  • Samydurai, P. and M. Saradha, Effects of Various Solvent on the Extraction of Antimicrobial, Antioxidant Phenolics from the Stem Bark of Decalepis hamiltonii Wight and Arn. Asian J. Res. Pharm, 2016. 6(2): 129-134. Doi: 10.5958/2231-5659.2016.00018.7


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  • In Vitro Assessment of Sun Protection Factor (Spf) and Antioxidant Activity of Viola Odorata Extracts

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Authors

Zeinab Sameer Ali
Master Student in Pharmaceutical Chemistry and Drug Control Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syrian Arab Republic
Dima Muhammad
Doctor in Pharmacognosy Department, Faculty of Pharmacy, Tishreen University, Lattakia,, Syrian Arab Republic
Afraa Zrieki
Doctor in Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Tishreen University, Lattakia, Syrian Arab Republic

Abstract


The harmful effects of sunlight on the skin have triggered several studies. They concentrated on the synthesis or extraction of natural compounds that can absorb ultraviolet (UV) radiation. In this study, we investigated the in vitro photo-protective potential of Viola odorata flowers extracts, in three states (fresh, dried, frozen), using three solvents (chloroform, ethyl acetate, ethanol) by sequential extraction. In vitro SPF values were determined by spectrophotometric method. Antioxidant activity was tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Total phenolic content (TPC) was measured using Folin-Ciocalteu method. The ethanolic extract fraction, at the concentration of 2 mg/ml, from the three flowers states had the best SPF values (27.249, 26.978, 27.907) respectively, followed by ethyl acetate extract fraction of dried flowers (SPF=21.11). The highest DPPH inhibition (about 85%) was also obtained in ethanolic extract fraction from the three states of flowers. The ethanolic extract fraction of fresh flowers showed the highest total phenolic content (18.575 mg GAE/g crud extract), followed by ethanolic extract fraction of dried flowers (15.06 mg GAE/g crud extract). There was a significant positive correlation between SPF values and each of antioxidant activity (R=0.92) and TPC (R=0.748) in the different extract fractions. Our results showed that ethnolic Viola odorata flowers extract, in different states, has a great potential to be used as a sunscreen agent due to its polyphenol content, which is also associated with strong antioxidant activity.

Keywords


SPF, Antioxidant, Polyphenols, Viola odorata, Extract.

References