Research Journal of Pharmacy and Technology

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Application of Ultrasound Assisted Extraction on Ripe Banana Peels (Musa Balbisiana Colla) and Its Potential as an Antioxidant and Antigout Agent


Affiliations
1 Department of Food Nanotechnology Politeknik AKA Bogor, Bogor 16158,, Indonesia
2 Department of Industrial Waste Treatment Politeknik AKA Bogor, Bogor 16158, Indonesia
3 Department of Analytical Chemistry Politeknik AKA Bogor, Bogor 16158,, Indonesia
     

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The utilization of stone bananas is still very minimal, especially banana peels which will only end up as waste. Despite the fact that stone bananas are a fruit high in metabolites, they have numerous health benefits.The banana peels (Musa balbisiana Colla) was extracted with ultrasound-assisted extraction to determine the content of secondary metabolites, total fernol, antioxidant capacity using the DPPH and FRAP methods, as well as the potential for antigout activity in the extract. Ultrasound-assisted extraction is a less expensive, simpler, and more reliable alternative to traditional extraction methods. Musa balbisiana Colla banana peel extract contains alkaloids, saponins, tannins, steroid glycosides and phenols as secondary metabolites. The results obtained from determining the total phenol content was 397.50±0.41 GAE/g sample. Then the results of testing for free radical scavenging activity with the DPPH and FRAP methods showed IC50 values of 143.97±0.17mg/L and 151.08± 0.32mg/L, respectively. Meanwhile the antigout activity was obtained 27.95±0.08% which was greater than Alupurinol, so that Musa balbisiana Colla banana peel has the potential as an alternative compound to reduce uric acid levels.

Keywords

Ultrasonic-Assisted Extraction, Banana Peels, Total Phenol, Antioxidant, Anti-gout.
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  • Prakash Maran J. Priya B. Naif Abdullah Al-Dhabi. Ponmurugan K. Ganesh Moorthy I. Sivarajasekar N. Ultrasound assisted citric acid mediated pectin extraction from industrial waste of Musa balbisiana. Ultrasonics Sonochemistry 2017; 35(1): 204-209. doi: 10.1016/j.ultsonch.2016.09.019.

  • Sharmila S. Ravi Teja P. Vijay Chandra Gangadhara Gupta D. Kaviya Lakshm P. Kowsalya E. Kamalambigeswari R. Jeyanthi Rebecca L. Production of Biodegradable Plastics using Starch and Waste Fruit Peels. Research J. Science and Tech 2021; 13(1):44- 48. doi: 10.5958/2349-2988.2021.00008.5

  • Subramaniyan R. Kiruthika N. Somanathan T. Sriraman V. Shanmugam M. High performance of Graphene oxide from Banana peel against Pseudomonas aeruginosa and Escherichia coli. Research J. Pharm. and Tech 2018; 11(7):2932-2934. doi: 10.5958/0974-360X.2018.00541.3

  • Jami’ah SR. Ifaya M. Pusparani J. Nurhikma E. Uji Aktivitas Antioksidan Ekstrak Metanol Kulit Pisang Raja (Musa paradisiaca sapientum) Dengan Metode DPPH (2,2-Difenil-1- Pikrilhidrazil). Jurnal Mandala Pharmacon Indonesia 2018; 4(1):33-38. doi: 10.35311/jmpi.v4i1.22

  • Alhabsyi DF. Aktivitas Antioksidan dan Tabir Surya pada Ekstrak Kulit Buah Pisang Goroho (Musa acuminate L.). Pharmacon 2014; 3(2):107-114. doi: 10.35799/pha.3.2014.4782

  • Candra F. Lister INE. Uji Aktivitas Antifungal Ekstrak Kulit Pisang Barangan (Musa acuminata Colla.) terhadap Pertumbuhan Jamur Pityrosporum Ovale. BioLink: Jurnal Biologi Lingkungan, Industri, Kesehatan 2019; 6(1):32-40. doi: 10.31289/biolink.v6i1.2211

  • Zeinab A. Salama, Ahmed M. Aboul-Enein, Alaa A. Gaafar, Mohsen S. Asker, Hanan F. Aly, Habiba A. Ahmed. In-vitro Antioxidant, Antimicrobial and Anticancer Activities of Banana leaves (Musa acuminata) and Olive leaves (Olea europaea L.) as by-products. Research J. Pharm. And Tech 2020; 13(2):687-696. doi: 10.5958/0974-360X.2020.00132.8

  • Wallace KL. Riedel AA. Joseph-Ridge N. Wortmann R. Increasing prevalence of gout and hyperuricemia over 10 years among older adults in a managed care population. The Journal of Rheumatology 2004; 31(8):1582-7. PMID: 15290739.

  • Sivakumar Muthusamy, Srikanth Jeyabalan. In-Silico and in-Vitro Evaluation of Xanthine Oxidase Inhibition of Zingiber officinalae for Hypouricemic activity. Research J. Pharm. and Tech 2019; 12(1): 314-320. doi: 10.5958/0974-360X.2019. 00057.X

  • C.Buvana, A. Sumathy, M. Sukumar. In silico Identification of Potential Xanthine Oxidase Inhibitors for the Treatment of Gout and Cardiovascular Disease. Asian J. Research Chem 2013; 6(11):1049-1053. Available on: https://ajrconline.org/AbstractView.aspx?PID=2013-6-11-13

  • Hudaib M. Tawaha K. Mohammad M. Assaf A. Issa A. Alali F. Aburjai T. Bustanji Y. Xanthine oxidase inhibitory activity of the methanolic extracts of selected Jordanian medicinal plants. Pharmacognosy Magazine 2011; 7(28):320-324. doi: 10.4103/0973-1296.90413

  • Teja K. Satyanarayana T. Saraswathi B. Goutham B. Mamatha K. Samyuktha P. Tharangini S. Phytochemical and In vitro Antiinflammatory Activity on Abrus precatorius. Asian J. Res. Pharm. Sci. 2019; 9(1):50-54. doi: 10.5958/2231-5659.2019.00008.0

  • Bhosle D. Janghel A. Deo S. Raut P. Verma C. Kumar SS. Agrawal M. Amit N. Sharma M. Giri T. Tripathi DK. Ajazuddin. Alexander A. Emerging Ultrasound Assisted Extraction (UAE) Techniques as Innovative Green Technologies for the effective extraction of the active phytopharmaceuticals. Research Journal of Pharmacy and Technology 2015; 8(7):963-970. doi: 10.5958/0974-360x.2015.00161.4

  • Wang L. Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology 2006; 17(6):300–12. doi: 10.1016/j.tifs.2005.12.004

  • Rostagno MA. Palma M. Barroso CG. Ultrasound-assisted extraction of soy isoflavones. Journal of Chromatography A 2003; 1012(2):119–128. doi: 10.1016/s0021-9673(03)01184-1

  • Ciulei I. Methodology for analysis of vegetable drugs. Chemical Industries Branch Division–Industrial Operation UNINDO, Bucharest–Romania. 1984; 11–23.

  • Singleton VL. Orthofer R. Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Oxidant and Antioxidant Part A, Methods in Enzymology. Elsevier. 1999;Vol 299:152–78. doi: 10.1016/s0076-6879(99)99017-1

  • Wang LY. Unehara N. Kitanaka S. Lignans from the roots of wikstroemia indica and their DPPH radical, scavenging and nitric oxide inhibitory activities. Chem Pharm. Bull. 2005;53(10):1348- 1351. doi: 10.1248/cpb.53.1348

  • Yefrida. Ulfaningsih M. Loekman U. Validasi metoda penentuan antioksidan total (dihitung sebagai asam sitrat) dalam Sampel jeruk secara spektrofotometri dengan menggunakan oksidator FeCl3 dan pengkompleks orto-fenantrolin. J.Ris.Kim 2014;7(2):186-193. doi: 10.25077/jrk.v7i2.187

  • Styani E. Irawan C. Hanafi H. Sulistiawaty L. Imalia I. Liquid Chromatograph – Mass Spectrophotometer and Anti Uric Acid Potential Studies of Ethyl Acetat Extract of Archidendron bubalinum (Jack) I.C. Nielsen Fruit Seed Shell. Proceedings of the International Conference on Science and Technology (ICST 2018) 2018; 1:293-297. doi: 10.2991/icst-18.2018.62

  • Melecchi, MIS. Péres, VF. Dariva, C. Zini, CA. Abad, FC. Martinez, MM. Caramão, EB. Optimization of the sonication extraction method of Hibiscus tiliaceus L. flowers. Ultrasonics Sonochemistry 2006; 13(3):242-250. doi: 10.1016/j.ultsonch.2005.02.003.

  • Kristanti, AN. Aminah NS. Tanjung M. Kurniadi B. Buku Ajar Fitokimia. Airlangga University Press., Surabaya. 2008; 23-47.

  • Jayashree P. Shalini M. Meenambiga SS. Suganya V. Phytochemical Screening and GC-MS Analysis of Epiphyllum oxypetalum flower extracts. Research J. Pharm. and Tech 2020; 13(12):5893-5897. doi: 10.5958/0974-360X.2020.01028.8

  • Khan AM. Bhadauria S. Yadav R. Phytochemical Screening and Antioxidant activity of extract of different parts of Adhatoda vasica. Research J. Pharm. and Tech 2019; 12(12): 5699-5705. doi: 10.5958/0974-360X.2019.00986.7

  • Sharma DR. Kumar U. Exploration and Phytochemical Estimation of Murraya koenigii Leaves for Pharmaceutical Applications. Asian J. Pharm. Res. 2019; 9(3):159-168. doi: 10.5958/2231- 5691.2019. 00025.X

  • Pourmorad, F. Hosseinimehr, SJ. Shahabimad N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnolog 2005; 5(1):1142-1145. Available online at http://www.academicjournals.org/AJB

  • Lee KW. Kim YJ. Lee HJ. Lee CY. Cocoa Has More Phenolic Phytochemical and A Higher Antioxidant Capacity than Teas and Red Wine. J. Agric. Food Chem 2003; 51(25): 7292-7295. doi: 10.1021/jf0344385

  • Huang D. Ou B. Prior RL. The chemistry behind antioxidant capacity assays. J. of Agricultural and Food Chemistry 2005; 53:1841- 1856. doi: 10.1021/jf030723c

  • Tursiman. Ardiningsih, P. Nofiani, R. Total Fenol Fraksi Etil Asetat dari Buah Asam Kandis (Garcinia dioica Blume). Jurnal Kimia Khatulistiwa 2012; 1(1):45-48. Available on: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/982/920

  • Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science Technology 2004; 26(2):211- 219. Available on: https://rdo.psu.ac.th/sjstweb/journal/26-2/07- DPPH.pdf

  • Muthia, R. Saputri, R. Verawati, SA. Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.) Menggunakan Metode DPPH (2,2-Diphenyl-1-picrylhydrazil). Jurnal Pharmascience 2019; 6(1):74-82. doi: 10.20527/jps.v6i1.6079

  • Wahdaningsih S. Budilaksono W. Fahrurroji A. Uji aktivitas antioksidan fraksi n-heksana kulit buah naga merah menggunakan metode 1,1-Difenil-2-Pikrilhidrazil. Jurnal Kesehatan Khatulistiwa 2015; 1(2):115-136. doi: 10.26418/jurkeswa.v1i2.42997

  • Fitrianingsih, SP. Lestari, F. Aminah, S. Uji Efek Antioksidan Ekstrak Etanol Kulit Buah Salak [Salacca zalacca (Gaertner) Voss] dengan Metode Peredaman DPPH. Prosiding SNaPP: Sains dan Teknologi 2014; 4(1)49-54. Available on: http://proceeding.unisba.ac.id/index.php/sains_teknologi/article/vi ew/538

  • Panda, SK. Assay guided comparison for enzymatic and nonenzymatic antioxidant activities with special reference to medicinal plants. In El-Missiry, M.A. (ed.), Antioxidant Enzyme. IntechOpen. 2012; 14:381-400. doi: 10.5772/50782

  • Apak R. Güçlü K. Demirata B. Özyürek M. Çelik SE. Bektaşoğlu B. Berker KI. Özyurt D. Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay. Molecules. 2007; 12(7):1496-1547. doi: 10.3390/12071496


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  • Application of Ultrasound Assisted Extraction on Ripe Banana Peels (Musa Balbisiana Colla) and Its Potential as an Antioxidant and Antigout Agent

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Authors

Imalia Dwi Putri
Department of Food Nanotechnology Politeknik AKA Bogor, Bogor 16158,, Indonesia
Candra Irawan
Department of Food Nanotechnology Politeknik AKA Bogor, Bogor 16158,, Indonesia
Maman Sukiman
Department of Industrial Waste Treatment Politeknik AKA Bogor, Bogor 16158, Indonesia
Lintannisa Rahmatia
Department of Analytical Chemistry Politeknik AKA Bogor, Bogor 16158,, Indonesia
Erna Styani
Department of Industrial Waste Treatment Politeknik AKA Bogor, Bogor 16158, Indonesia
Aulia Novianti
Department of Food Nanotechnology Politeknik AKA Bogor, Bogor 16158,, Indonesia
Avisani Dewanta
Department of Analytical Chemistry Politeknik AKA Bogor, Bogor 16158,, Indonesia

Abstract


The utilization of stone bananas is still very minimal, especially banana peels which will only end up as waste. Despite the fact that stone bananas are a fruit high in metabolites, they have numerous health benefits.The banana peels (Musa balbisiana Colla) was extracted with ultrasound-assisted extraction to determine the content of secondary metabolites, total fernol, antioxidant capacity using the DPPH and FRAP methods, as well as the potential for antigout activity in the extract. Ultrasound-assisted extraction is a less expensive, simpler, and more reliable alternative to traditional extraction methods. Musa balbisiana Colla banana peel extract contains alkaloids, saponins, tannins, steroid glycosides and phenols as secondary metabolites. The results obtained from determining the total phenol content was 397.50±0.41 GAE/g sample. Then the results of testing for free radical scavenging activity with the DPPH and FRAP methods showed IC50 values of 143.97±0.17mg/L and 151.08± 0.32mg/L, respectively. Meanwhile the antigout activity was obtained 27.95±0.08% which was greater than Alupurinol, so that Musa balbisiana Colla banana peel has the potential as an alternative compound to reduce uric acid levels.

Keywords


Ultrasonic-Assisted Extraction, Banana Peels, Total Phenol, Antioxidant, Anti-gout.

References