Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Increased Amount of Blood Vessels after Blue-light-emitting Diode Exposure During Orthodontic Tooth Movement in Rats


Affiliations
1 Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281,, Indonesia
2 Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281, ., Indonesia
     

   Subscribe/Renew Journal


Orthodontic tooth movement (OTM) results from a biological response that facilitates tooth movement. Light-emitting diode (LED) can produce a variety of beneficial biological effects. Angiogenesis plays an important role in bone remodeling; hence, the amount of blood vessels can be used as a benchmark in OTM. The objective of this study is to analyze the effects of blue LED on the amount of blood vessels visible on the tension and pressure side during OTM. This study included 48 Wistar rats that were divided into 4 groups: control group; the treatment group exposed to blue LED light for 25 seconds (T1); the treatment group exposed to blue LED light for 30 seconds (T2); and the treatment group exposed to blue LED light for 35 seconds (T3) (n = 12). An open coil spring placed on the mandibular incisors generated the orthodontic force. The amount of blood vessels present on the pressure and tension sides of the periodontal tissue was observed on histological specimens with hematoxylin–eosin staining on days 0, 3, 7, and 14 at 400× magnification. The obtained data were analyzed using two-way ANOVA followed by the post-hoc least significant different (LSD) test (P < 0.05). The amount of blood vessels on the tension and pressure sides in the T3 treatment group was higher than the other groups (P < 0.05). We conclude that exposure to blue LED increased the amount of blood vessels during OTM on the tension and pressure sides in rats.

Keywords

Orthodontic, Blue-light-emitting diode, Remodeling, Accelerated tooth movement side, Blood vessels.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Nugraha AP, Narmada IB, Sitasari PI, Inayati F, Wira R, Triwardhani A, et al. High mobility group box 1 and heat shock protein-70 expression post (-)-epigallocatechin-3-gallate in East Java green tea methanolic extract administration during orthodontic tooth movement in wistar rats. Pesqui Bras Odontopediatria Clín Integr. 2020; 20:e5347. doi:10.1590/pboci.2020.040

  • Shahana RY, Muralidharan NP. Efficacy of mouth rinse in maintaining oral health of patients attending orthodontic clinics. Research J. Pharm. and Tech 2016; 9(11): 1991–1993. doi: 10.5958/0974-360X.2016.00406.6

  • Aravind N, Saravana Pandiyan. Demineralisation around orthodontic brackets- a review. Research J. Pharm. and Tech 2016; 9(9):1536-1540. doi: 10.5958/0974-360X.2016.00301.2

  • Arianda TA, Rezqita P, Pudyani PS, Rosyida NF, Alhasyimi AA. Effect of Cocoa Administration on Osteoblast Counts and Alkaline Phosphatase Levels During orthodontic tooth movement in rats. J Orofac Sci 2020; 12: 101–106. doi: 10.4103/jofs.jofs_51_20

  • Alhasyimi AA, Rosyida NF. Cocoa administration may accelerate orthodontic tooth movement by inducing osteoclastogenesis in rats. Iran J Basic Med Sci 2019; 22(2): 206–210. doi: 10.22038/ijbms.2018.32967.7881.

  • Ekizer A, Türker G, Uysal T, Güray E, Taşdemir Z. Light emitting diode mediated photobiomodulation therapy improves orthodontic tooth movement and miniscrew stability: A randomized controlled clinical trial. Lasers Surg Med 2016; 48(10): 936–943. doi: 10.1002/lsm.22516

  • Ubolviroj C, Komoltri C, Manopattanakul S, Viwattanatipa N. Effect of light emitting diodes (LED) with 430–480 nm wavelength Upon tooth movement. Biomed J Sci Tech Res 2018; 5(4): 7778–7786. doi: 10.26717/BJSTR.2018.10.001947

  • ChaveChaves ME, Araújo AR, Piancastelli AC. Pinotti M. Effects of low-power light therapy on wound healing: Laser X LED. An Bras Dermatol 2014; 89(4): 616–623. doi: 10.1590/abd1806-4841.20142519

  • Triwardhani A, Oktaviona I, Narmada IB, Nugraha AP, Riawan W. The effect of bifidobacterium probiotic on heat shock protein-70 expression and osteoclast number during orthodontic tooth movement in rats (Rattus novergicus). Research J. Pharm. and Tech 2021; 14(3): 1477–1481. doi: 10.5958/0974-360X.2021.00262.6

  • Hermawan RW, Narmada IB, Djaharu’ddin I, Nugraha AP, Rahmawati D. The influence of epigallocatechin gallate on the nuclear factor associated t cell-1 and sclerostin expression in Wistar rats (Rattus novergicus) during the orthodontic tooth movement. Research J. Pharm. and Tech 2020; 13(4): 1730–1734. doi: 10.5958/0974-360X.2020.00312.1

  • Indriasari V, Suparwitri S, Christnawati C, Alhasyimi AA. Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits. F1000Research 2020; 8: 2074. doi: 10.12688/f1000research.21211.2

  • Hisham PBBM, Narmada IB, Alida A, Rahmawati D, Nugraha AP, Putranti NA. Effects of Vitamin D in alveolar bone remodeling on osteoblast numbers and bone alkaline phosphatase expression in pregnant rats during orthodontic tooth movement. J Orofac Sci 2019;11:79-83. doi: 10.4103/jofs.jofs_10_19

  • Borovkova E, Schegolev A. Vascular endothelial growth factor and its receptors in the placenta of gravidas with obesity. Research J. Pharm. and Tech. 2018; 11(3): 1153-1158. doi: 10.5958/0974-360X.2018.00215.9

  • Bilal M, Al-Saleh J, Fakher FA. Serum levels of prostaglandin E2 (PGE2) and interleukin 17 (IL-17) are associated with angiogenesis and metastasis in breast cancer patients. Research J. Pharm. and Tech 2021; 14(1): 317–320. doi: 10.5958/0974-360X.2021.00058.5

  • Kau CH, Kantarci A, Shaughnessy T, Vachiramon A, Santiwong P, Fuente ADL, Skrenes D, Ma D, Brawn P. Photobiomodulation accelerates orthodontic alignment in the early phase of treatment. Prog Orthod 2013; 14(30): 1–9. doi: 10.1186/2196-1042-14-30.

  • Rohringer S, Holnthoner W, Chaudary S, Slezak P, Priglinger E, Strassl M, Pill K, Mühleder S, Redl H, Dungel P. The impact of wavelengths of LED light-therapy on endothelial cells. Sci Rep 2017; 7(1): 10700. doi: 10.1038/s41598-017-11061-y.

  • Dungel P, Hartinger J, Chaudary S, Slezak P, Hofmann A, Hausner T, Strassl M, Wintner E, Redl H, Mittermayr R. Low level light therapy by LED of different wavelength induces angiogenesis and improves ischemic wound healing. Lasers Surg Med 2014; 46(10): 773–780. doi: 10.1002/lsm.22299.

  • Nareswari RAAR, Narmada IB, Djaharu’ddin I, Rahmawati D, Putranti NAR, Nugraha AP. Effect of vitamin D administration on vascular endothelial growth factor expression and angiogenesis number in orthodontic tooth movement of pregnant Wistar rats. J Postgrad Med Inst 2019; 33(3): 182-8.

  • Asiry MA. Biological aspects of orthodontic tooth movement: A review of literature. Saudi J Biol Sci 2018; 25(6): 1027–1032. doi: 10.1016/j.sjbs.2018.03.008

  • Inayati F, Narmada IB, Ardani IGAW, Nugraha AP, Rahmawati D. Post oral administration of epigallocatechin gallate from camelia sinensis extract enhances vascular endothelial growth factor and fibroblast growth factor expression during orthodontic tooth movement in wistar rats. JKIMSU 2020;9(1):58-65.

  • Jain NK, Baghel KS. Selective cyclooxygenase-2 inhibitor etoricoxib attenuated hypoxic cancer milieu induced m2-polarization of macrophages and acquisition of pro-angiogenic and pro-invasive attributes. Research J. Pharm. and Tech. 2019; 12(12): 5871-5877. doi: 10.5958/0974-360X.2019.01018.7.

  • Zein R, Selting W, Hamblin MR. Review of light parameters and photobiomodulation efficacy: Dive into complexity. J Biomed Opt 2018; 23(12): 1–17; 30550048. doi: 10.1117/1.JBO.23.12.120901.


Abstract Views: 154

PDF Views: 0




  • Increased Amount of Blood Vessels after Blue-light-emitting Diode Exposure During Orthodontic Tooth Movement in Rats

Abstract Views: 154  |  PDF Views: 0

Authors

Efraim Herisman Satyaputra
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281,, Indonesia
Dyah Karunia
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281,, Indonesia
Darmawan Soetantyo
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281,, Indonesia
Ananto Ali Alhasyimi
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281, ., Indonesia

Abstract


Orthodontic tooth movement (OTM) results from a biological response that facilitates tooth movement. Light-emitting diode (LED) can produce a variety of beneficial biological effects. Angiogenesis plays an important role in bone remodeling; hence, the amount of blood vessels can be used as a benchmark in OTM. The objective of this study is to analyze the effects of blue LED on the amount of blood vessels visible on the tension and pressure side during OTM. This study included 48 Wistar rats that were divided into 4 groups: control group; the treatment group exposed to blue LED light for 25 seconds (T1); the treatment group exposed to blue LED light for 30 seconds (T2); and the treatment group exposed to blue LED light for 35 seconds (T3) (n = 12). An open coil spring placed on the mandibular incisors generated the orthodontic force. The amount of blood vessels present on the pressure and tension sides of the periodontal tissue was observed on histological specimens with hematoxylin–eosin staining on days 0, 3, 7, and 14 at 400× magnification. The obtained data were analyzed using two-way ANOVA followed by the post-hoc least significant different (LSD) test (P < 0.05). The amount of blood vessels on the tension and pressure sides in the T3 treatment group was higher than the other groups (P < 0.05). We conclude that exposure to blue LED increased the amount of blood vessels during OTM on the tension and pressure sides in rats.

Keywords


Orthodontic, Blue-light-emitting diode, Remodeling, Accelerated tooth movement side, Blood vessels.

References