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Phytochemical Screening and Antioxidant Activity of Cananga Odorata Extract


Affiliations
1 Faculty of Pharmacy, AIMST University, Kedah 08100,, Malaysia
2 Faculty of Pharmacy, AIMST University, Kedah 08100, Malaysia
3 Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
4 Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh 30450, Malaysia
5 Faculty of Applied Science, AIMST University, Kedah 08100,, Malaysia
6 Faculty of Bio-economy and Health Sciences, Geomatika University College Kuala Lumpur 54200,, Malaysia
7 Centre for Virus and Vaccine Research & Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, Subang Jaya, Selangor,, Malaysia
8 Faculty of Pharmacy, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
9 Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia
10 Faculty of Pharmacy, AIMST University, Kedah 08100,

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Plants are the natural source for the antioxidants from plants source is boon for the investigators. Present study was aimed to study the antioxidant activity and phytochemical screening of Cananga odorata (Lam.) Hook. f. & Thomson extract. Study involved preparation hydroalcoholic extract of Cananga odorata leaves (HECOL) using maceration method, followed by its phytochemical screening and antioxidant activity. The HECOL was subjected to determination of total phenolic content (TPC) and total flavonoid content (TFC), and DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging assay for determination of antioxidant activity. Maceration of C. odorata using hydroalcoholic solvent offered dark brown colored HECOL. Phytochemical screening of HECOL exhibited presence of carbohydrates, mucilage, fats and oils, volatile oils, anthraquinone glycosides, flavonoids, alkaloids, tannins and phenolic compounds. For TPC the gallic acid equivalent of HECOL was found to be 0.507 mg GAE/g. Whereas for TFC, the rutin equivalent of HECOL was found to be 0.2224mg RE/g. The antioxidant activity of HECOL was estimated to be 120.44μg/ml (IC50). Based on the experimental results, present study concludes that HECOL possess the significant antioxidant potential. This study also recommends that antioxidant activity potential of C. odorata leaves should be further explored using different solvent extracts.

Keywords

Canaga odorata, total phenolic content, gallic acid, total flavonoid content, antioxidant activity.
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Abstract Views: 117




  • Phytochemical Screening and Antioxidant Activity of Cananga Odorata Extract

Abstract Views: 117  | 

Authors

Chew Khe Jiea
Faculty of Pharmacy, AIMST University, Kedah 08100,, Malaysia
Shivkanya Fuloria
Faculty of Pharmacy, AIMST University, Kedah 08100, Malaysia
Vetriselvan Subrimanyan
Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Mahendran Sekar
Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh 30450, Malaysia
Kathiresan V. Sathasivam
Faculty of Applied Science, AIMST University, Kedah 08100,, Malaysia
Saminathan Kayarohanam
Faculty of Bio-economy and Health Sciences, Geomatika University College Kuala Lumpur 54200,, Malaysia
Yuan Seng Wu
Centre for Virus and Vaccine Research & Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, Subang Jaya, Selangor,, Malaysia
Venkata Sathya Saiappala Raju Velaga
Faculty of Pharmacy, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Ashok Kumar Janakiraman
Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia
Mohammad Nazmul Hasan Maziz
Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Neeraj Kumar Fuloria
Faculty of Pharmacy, AIMST University, Kedah 08100,

Abstract


Plants are the natural source for the antioxidants from plants source is boon for the investigators. Present study was aimed to study the antioxidant activity and phytochemical screening of Cananga odorata (Lam.) Hook. f. & Thomson extract. Study involved preparation hydroalcoholic extract of Cananga odorata leaves (HECOL) using maceration method, followed by its phytochemical screening and antioxidant activity. The HECOL was subjected to determination of total phenolic content (TPC) and total flavonoid content (TFC), and DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging assay for determination of antioxidant activity. Maceration of C. odorata using hydroalcoholic solvent offered dark brown colored HECOL. Phytochemical screening of HECOL exhibited presence of carbohydrates, mucilage, fats and oils, volatile oils, anthraquinone glycosides, flavonoids, alkaloids, tannins and phenolic compounds. For TPC the gallic acid equivalent of HECOL was found to be 0.507 mg GAE/g. Whereas for TFC, the rutin equivalent of HECOL was found to be 0.2224mg RE/g. The antioxidant activity of HECOL was estimated to be 120.44μg/ml (IC50). Based on the experimental results, present study concludes that HECOL possess the significant antioxidant potential. This study also recommends that antioxidant activity potential of C. odorata leaves should be further explored using different solvent extracts.

Keywords


Canaga odorata, total phenolic content, gallic acid, total flavonoid content, antioxidant activity.

References