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Phytochemical Screening and Antioxidant Activity of Cananga Odorata Extract


Affiliations
1 Faculty of Pharmacy, AIMST University, Kedah 08100,, Malaysia
2 Faculty of Pharmacy, AIMST University, Kedah 08100, Malaysia
3 Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
4 Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh 30450, Malaysia
5 Faculty of Applied Science, AIMST University, Kedah 08100,, Malaysia
6 Faculty of Bio-economy and Health Sciences, Geomatika University College Kuala Lumpur 54200,, Malaysia
7 Centre for Virus and Vaccine Research & Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, Subang Jaya, Selangor,, Malaysia
8 Faculty of Pharmacy, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
9 Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia
10 Faculty of Pharmacy, AIMST University, Kedah 08100,

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Plants are the natural source for the antioxidants from plants source is boon for the investigators. Present study was aimed to study the antioxidant activity and phytochemical screening of Cananga odorata (Lam.) Hook. f. & Thomson extract. Study involved preparation hydroalcoholic extract of Cananga odorata leaves (HECOL) using maceration method, followed by its phytochemical screening and antioxidant activity. The HECOL was subjected to determination of total phenolic content (TPC) and total flavonoid content (TFC), and DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging assay for determination of antioxidant activity. Maceration of C. odorata using hydroalcoholic solvent offered dark brown colored HECOL. Phytochemical screening of HECOL exhibited presence of carbohydrates, mucilage, fats and oils, volatile oils, anthraquinone glycosides, flavonoids, alkaloids, tannins and phenolic compounds. For TPC the gallic acid equivalent of HECOL was found to be 0.507 mg GAE/g. Whereas for TFC, the rutin equivalent of HECOL was found to be 0.2224mg RE/g. The antioxidant activity of HECOL was estimated to be 120.44μg/ml (IC50). Based on the experimental results, present study concludes that HECOL possess the significant antioxidant potential. This study also recommends that antioxidant activity potential of C. odorata leaves should be further explored using different solvent extracts.

Keywords

Canaga odorata, total phenolic content, gallic acid, total flavonoid content, antioxidant activity.
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  • Malviya R, Jha S, Fuloria NK et al. Determination of temperature-dependent coefficients of viscosity and surface tension of tamarind seeds (Tamarindus indica L.) polymer. Polymers. 2021;13(4):610. doi: 10.3390/polym13040610

  • Fuloria NK, Fuloria S, Sharma VK et al. Isolation of new diterpene from methanolic extract of Capsicum annuum Linn. fruits. Pharmacognosy Magazine. 2020;16(72):730. doi: 10.4103/pm.pm_250_20

  • Chinni SV, Gopinath SC, Anbu P et al. Characterization and antibacterial response of silver nanoparticles biosynthesized using an ethanolic extract of Coccinia indica leaves. Crystals. 2021;11(2):97. doi: 10.3390/cryst11020097

  • Fuloria NK, Ko MY, Rui CS, et al. Green synthesis and evaluation of dimocarpus longan leaves extract based chitosan nanoparticles against periodontitis triggering bacteria. Asian Journal of Chemistry. 2020;32(7):1660-1666. doi: 10.14233/ajchem.2020.22649

  • Velu V, Fuloria N, Fuloria S, et al. In Vitro and In Vivo Anti-urolithiatic activity of terpenoid-rich ethyl acetate extract of rhizomes of Curcuma zedoaria. studies on ethno medicine. 2018; 12(1,2): 31-39. doi:10.31901/24566772.2018/12.01.532

  • Vakiloddin S, Fuloria N, Fuloria S, et al. Evidences of hepatoprotective and antioxidant effect of Citrullus colocynthis fruits in paracetamol induced hepatotoxicity. Pakistan Journal of Pharmaceutical Sciences. 2015;28(3):951-957.

  • Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology. 2014;4: 177. doi: 10.3389/fphar.2013.00177

  • Venkateshan S, Subramaniyan V, Chinnasamy V, et al. Anti-oxidant and anti-hyperlipidemic activity of Hemidesmus indicus in rats fed with high-fat diet. Avicenna J Phytomed. 2016; 6(5):516-525.

  • Fuloria S, Subramaniyan V, Karupiah S, et al. Comprehensive review on source, types, effects, nanotechnology, detection, and therapeutic management of reactive carbonyl species associated with various chronic diseases. Antioxidants 2020; 9, 1075. doi: 10.3390/antiox9111075

  • Fuloria S, Subramaniyan V, Karupiah S, et al. Comprehensive Review of Methodology to Detect Reactive Oxygen Species (ROS) in Mammalian Species and Establish Its Relationship with Antioxidants and Cancer. Antioxidants. 2021;10(1):128. doi:10.3390/antiox9111075

  • Fuloria S, Wei LT, Karupiah S, et al. Development and validation of UV-visible method to determine gallic acid in hydroalcoholic extract of Erythrina fusca Leaves. 2020;11(4), 6319-6326. doi: 10.26452/ijrps.v11i4.3386

  • Saminathan Kayarohanam, Vetriselvan Subramaniyan, Ashok Kumar Janakiraman, Madhan Kumar S. J. Antioxidant, Antidiabetic, and Antihyperlipidemic Activities of Dolichandrone atrovirens in Albino Wistar rats. Research J. Pharm. and Tech. 2019; 12(7): 3511-3516. doi: 10.5958/0974-360X.2019.00597.3

  • Jaju SB, Indurwade NH, Sakarkar DM, Fuloria NK, Ali MD, Basu SP. Isolation of β-sitosterol diglucosyl caprate from Alpinia galanga. Pharmacognosy research. 2010;2(4):264-266. doi: 10.4103/0974-8490.69129

  • Jaju SB, Indurwade NH, Sakarkar DM, Fuloria NK, Ali MD, Das S, Basu SP. Galangoflavonoid isolated from rhizome of Alpinia galanga (L) Sw (Zingiberaceae). Tropical Journal of Pharmaceutical Research. 2009;8(6):545-550.

  • Jaju S, Indurwade N, Sakarkar D, Fuloria N, Al M. Isolation of galangogalloside from rhizomes of Alpinia galanga. International Journal of Green Pharmacy. 2009;3(2):144-147.

  • Jude Chigozie Ikewuchi, Catherine Chidinma Ikewuchi, Mercy Onuekwuzu Ifeanacho. An Aqueous Extract of the Leaves of Chromolaena odorata Moderated Plasma Biochemical and Hematological Indices of Sub-Chronic Salt-Loaded Rats. Asian J. Pharm. Res.2014; 4(1): 24-35.

  • Chinnasamy V, Subramaniyan V, Chandiran S, et al. Antiarthritic Activity of Achyranthes Aspera on Formaldehyde - Induced Arthritis in Rats. Open Access Maced J Med Sci. 2019;7(17):2709-2714. doi: 10.3889/oamjms.2019.559.

  • Vetriselvan Subramaniyan, Saminathan Kayarohanam, Ashok Kumar J, et al. Impact of herbal drugs and its clinical application. International Journal of Research in Pharmaceutical Sciences, 10(2), 1340-1345.

  • Patel Jitendra, Qureshi Md Shamim, Kumar GS, Panigrahy Uttam Prasad. Phytochemical and Pharmacological activities of Eupatorium odoratum L. Research J. Pharm. and Tech. 4(2): 2011; 184-188.

  • Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese medicine. 2018;13(1):1-26. doi: 10.1186/s13020-018-0177-x

  • Altemimi A, Lakhssassi N, Baharlouei A, et al. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017;6(4):42. doi: 10.3390/plants6040042

  • Fuloria NK, Fuloria S, Chia KY, Karupiah S, Sathasivam K. Response of green synthesized drug blended silver nanoparticles against periodontal disease triggering pathogenic microbiota. Journal of Applied Biology and Biotechnology. 2019;7:46-56. doi: 10.7324/JABB.2019.70408

  • Shubhangi NP, Neha DT, Shubhangi HC, et al. To study extraction, phytochemical screening and formulation from Stevia rebaudiana bertoni. Research Journal of Pharmacy and Technology. 2020; 13(12):5757-5762. doi: 10.5958/0974-360X.2020.01003.3

  • Trease GE, Evan WC. Pharmacognosy. 11th edn. Brailliar Tridel Can. Macmillan Publishers.1989.

  • Krishna Kondragunta. V, Karuppuraj. V, Perumal. K. Antioxidant activity and Folic acid content in indigenous isolates of Ganoderma lucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.

  • Shantaram M, Bollapragada M. Comparative phytochemical profiles and antioxidant properties of Antigonon leptopus, Artabotrys hexapetalus and Allamanda blanchetii leaf extracts. International Journal of Pharmacognosy and Phytochemical Research. 2018;10(10):327-33. doi: 10.25258/phyto.10.10.2

  • K Amutha , D Victor Arokia Doss. In Vitro Antioxidant Activity of Ethanolic Extract of Barleria cristata L. Leaves. Research J. Pharmacognosy and Phytochemistry 2009; 1(3): 209-212.

  • Debashisha Panda, Santosh Kumar Dash, Gouri Kumar Dash. Preliminary phytochemical screening and Pharmacogcostic studies of the common weeds, Mimosa pudica Linn., Chromolaena odorata Linn. and Tragia involucrata Linn. collected from Ganjam District, Odisha: A Comparative Study. Research J. Pharmacognosy and Phytochemistry 2012; 4(2): 92-96.

  • Yahaya MF, Kubmarawa D, Yelwa JM, Runde M. Antioxidant and antimicrobial activity of essential oils extracted from aromatic plants. World Sci News. 2018;111: 13–25.

  • Ramya Kuber B. In vitro Antioxidant potential, Total Phenolic and Flavonoid contents in Justicia gendarussa leaf extracts. Research Journal of Pharmacy and Technology. 2021; 14(5):2707-3. doi: 10.52711/0974-360X.2021.00477

  • Som K. Madhvi, Manik Sharma, Javaid Iqbal, Mohd Younis, Razda Sheikh. Phytochemical analysis, Total Flavonoid, Phenolic contents and Antioxidant activity of extracts from the Leaves of Rhododendron arboreum. Research J. Pharm. and Tech. 2020; 13(4):1701-1706. doi: 10.5958/0974-360X.2020.00307.8

  • Côté J, Caillet S, Doyon G, Sylvain J-F, Lacroix M. Bioactive compounds in cranberries and their biological properties. Critical Reviews in Food Science and Nutrition. 2010; 50(7): 666–79. doi: 10.1080/10408390903044107

  • Babbar N, Oberoi HS, Sandhu SK. Therapeutic and nutraceutical potential of bioactive compounds extracted from fruit residues. Critical Reviews in Food Science and Nutrition. 2015; 55(3):319-37. doi: 10.1080/10408398.2011.653734

  • Yamin, Ruslin, Ari Sartinah, Sunandar Ihsan, Henny Kasmawati, Suryani, Rina Andriyani, Asma, Andi Nafisah Tendri Adjeng, Muhammad Arba. Radical scavenging assay and determination Flavonoid and Phenolic total of extract and Fractions of Raghu bark (Dracontomelon dao (Blanco) Merr). Research J. Pharm. and Tech 2020; 13(5): 2335-2339. doi: 10.5958/0974-360X.2020.00420.5

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Abstract Views: 196




  • Phytochemical Screening and Antioxidant Activity of Cananga Odorata Extract

Abstract Views: 196  | 

Authors

Chew Khe Jiea
Faculty of Pharmacy, AIMST University, Kedah 08100,, Malaysia
Shivkanya Fuloria
Faculty of Pharmacy, AIMST University, Kedah 08100, Malaysia
Vetriselvan Subrimanyan
Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Mahendran Sekar
Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh 30450, Malaysia
Kathiresan V. Sathasivam
Faculty of Applied Science, AIMST University, Kedah 08100,, Malaysia
Saminathan Kayarohanam
Faculty of Bio-economy and Health Sciences, Geomatika University College Kuala Lumpur 54200,, Malaysia
Yuan Seng Wu
Centre for Virus and Vaccine Research & Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, Subang Jaya, Selangor,, Malaysia
Venkata Sathya Saiappala Raju Velaga
Faculty of Pharmacy, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Ashok Kumar Janakiraman
Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia
Mohammad Nazmul Hasan Maziz
Faculty of Medicine, Bioscience and Nursing, MAHSA University, Bandar Saujana Putra 42610,, Malaysia
Neeraj Kumar Fuloria
Faculty of Pharmacy, AIMST University, Kedah 08100,

Abstract


Plants are the natural source for the antioxidants from plants source is boon for the investigators. Present study was aimed to study the antioxidant activity and phytochemical screening of Cananga odorata (Lam.) Hook. f. & Thomson extract. Study involved preparation hydroalcoholic extract of Cananga odorata leaves (HECOL) using maceration method, followed by its phytochemical screening and antioxidant activity. The HECOL was subjected to determination of total phenolic content (TPC) and total flavonoid content (TFC), and DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging assay for determination of antioxidant activity. Maceration of C. odorata using hydroalcoholic solvent offered dark brown colored HECOL. Phytochemical screening of HECOL exhibited presence of carbohydrates, mucilage, fats and oils, volatile oils, anthraquinone glycosides, flavonoids, alkaloids, tannins and phenolic compounds. For TPC the gallic acid equivalent of HECOL was found to be 0.507 mg GAE/g. Whereas for TFC, the rutin equivalent of HECOL was found to be 0.2224mg RE/g. The antioxidant activity of HECOL was estimated to be 120.44μg/ml (IC50). Based on the experimental results, present study concludes that HECOL possess the significant antioxidant potential. This study also recommends that antioxidant activity potential of C. odorata leaves should be further explored using different solvent extracts.

Keywords


Canaga odorata, total phenolic content, gallic acid, total flavonoid content, antioxidant activity.

References