Research Journal of Pharmacy and Technology

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Multi Epitopes Potential on Surface Sars-cov-2 Protein as a Covid-19 Vaccine Candidate


Affiliations
1 Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
2 Department of Emergency Medicine, Dr. Iskak General Hospital, Tulungagung, Indonesia
3 Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia
4 Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia
5 Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya Malang, Indonesia
     

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Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the etiology of an outbreak Covid-19. SARS-CoV-2 has a structural part consisting of spike glycoprotein, nucleoprotein N, membrane M and envelopes small membrane pentamer E. Immunoinformatic approach epitope analysis is developed to identify both weak and robust epitopes. Our study aims to identify several epitopes present in the spike glycoprotein, envelope, and membrane protein from the SARCoV-2 surface, with the help of insilico approach that highly potential as vaccine candidates. Analysis of antigeninicity was performed with the Kolaskar and Tongaonkar Antigenicity software. Epitope Mapping was analyzed using Linear Epitope Prediction Bepired. The structure of proteins with epitope regions was visualized by software Pyrex and PyMOL. Conserve analysis was performed using bio edit software. HLA mimicry was analyzed through HLAPred software. Molecular docking between the epitope with HLA I and HLA II was validated by Chimera and PyMOL software. The toxicity test for candidate vaccine peptides was carried out using ToxinPred software. Our study found seven potential epitope candidates as vaccine candidates. The seven epitopes were derived from spike proteins (5 epitopes), envelope proteins (1 epitope), and membrane proteins (1 epitope). All epitope codes are conserved and are not the same as HLA in Humans. The docking test results show a value with low affinity so that a strong bond can provide a high immune response. Toxicity tests show that all epitopes are non-toxic and safe to use as vaccine ingredients. Seven peptides from the spike, envelope, membrane protein that showed potential as vaccine candidates against Covid-19.

Keywords

Immunoinformatic, Surface protein, Epitope, Covid-19, Vaccine candidate.
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  • Multi Epitopes Potential on Surface Sars-cov-2 Protein as a Covid-19 Vaccine Candidate

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Authors

Khoirul Anam
Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Bobi Prabowo
Department of Emergency Medicine, Dr. Iskak General Hospital, Tulungagung, Indonesia
Meike Tiya Kusuma
Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia
Yuliati
Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia
Sri Winarsih
Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia
Tri Yudani Mardining Raras
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya Malang, Indonesia
Sumarno Reto Prawiro
Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang, Indonesia

Abstract


Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the etiology of an outbreak Covid-19. SARS-CoV-2 has a structural part consisting of spike glycoprotein, nucleoprotein N, membrane M and envelopes small membrane pentamer E. Immunoinformatic approach epitope analysis is developed to identify both weak and robust epitopes. Our study aims to identify several epitopes present in the spike glycoprotein, envelope, and membrane protein from the SARCoV-2 surface, with the help of insilico approach that highly potential as vaccine candidates. Analysis of antigeninicity was performed with the Kolaskar and Tongaonkar Antigenicity software. Epitope Mapping was analyzed using Linear Epitope Prediction Bepired. The structure of proteins with epitope regions was visualized by software Pyrex and PyMOL. Conserve analysis was performed using bio edit software. HLA mimicry was analyzed through HLAPred software. Molecular docking between the epitope with HLA I and HLA II was validated by Chimera and PyMOL software. The toxicity test for candidate vaccine peptides was carried out using ToxinPred software. Our study found seven potential epitope candidates as vaccine candidates. The seven epitopes were derived from spike proteins (5 epitopes), envelope proteins (1 epitope), and membrane proteins (1 epitope). All epitope codes are conserved and are not the same as HLA in Humans. The docking test results show a value with low affinity so that a strong bond can provide a high immune response. Toxicity tests show that all epitopes are non-toxic and safe to use as vaccine ingredients. Seven peptides from the spike, envelope, membrane protein that showed potential as vaccine candidates against Covid-19.

Keywords


Immunoinformatic, Surface protein, Epitope, Covid-19, Vaccine candidate.

References