Research Journal of Pharmacy and Technology

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Evaluation of Anti-oxidant, Anti-microbial and Phytochemical Analysis of Different Parts of C. Bipinatus


Affiliations
1 Department of Biotechnology, GIT, GITAM Deemed to be University, Visakhapatnam, India, 530045., India
2 Assistant Professor, Department of Biotechnology, GITAM Institute of Technology, GITAM University. Visakhapatnam,, India
     

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Plants are sources of many natural active compounds used to prevent several diseases. The various parts of plants like leaf, stem, flower and root are rich of these bioactive compounds. From ancient times these natural compounds have been using for treatment and prevention of diseases. Cosmos bipinnatus is an herbaceous, medicinal plant. The present study was aimed with the objective to antioxidant, antimicrobial and phytochemical screening, total phenolic and total flavonoid content of different solvent extracts of different parts of Cosmos bipinnatus. Flavonoids were abundantly found in the leaves and the root of Cosmos bipinnatus. Steroids were absent only in the leaves and flowers of Cosmos bipinnatus while terpenoids were completely absent in the flowers of the plant. Alkaloids were absent in the leaves extracted from all the three solvents and root sample extracted using ethyl acetate and stem sample extracted using ethanol. Proteins are absent in all the parts of the plant extracted using all the three solvents namely ethanol, ethyl acetate and hexane. Tannins are absent in the entirely in the flower in addition to the stem sample taken using the solvent hexane. Saponins are present all the parts like leaf, stem and flower but are not found in the root extract of the plant. The total phenolic and flavonoid contents of the extracts were calculated by using the standard gallic acid and quercetin graphs to find out the concentration. The total phenolic content is high for the plant sample stem that is extracted by using hexane as the solvent the value is 790μg/mg. The total flavonoid content is high for the plant sample stem that is extracted by using hexane as the solvent the value is 150μg/mg. The antioxidant activity was also found out by using various assays like FRAP and DPPH where the percentage of inhibition was found out. The highest percentage of inhibition for leaf was found out to be at 120μg/ml with the solvent hexane that is 88.86%. The highest percentage of inhibition for stem was found out to be at 60mg/ml using ethanol that is 89.92%. The highest percentage of inhibition for flower was found out to be at 60mg/ml by ethyl acetate that is 91.42%. The highest percentage of inhibition for root was found out to be at 60mg/ml that is 97%. From the FRAP results it is evident that the antioxidant activity is highest for the root sample.

Keywords

Phytochemical analysis, Phenolic content, flavonoid, antioxidant, antimicrobial activity.
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  • Ujjwal N. Ruchi S. Rashmi KM. Raju KC. Lipid Content and in vitro Antimicrobial Activity of Oil Seeds of Some Indian Medicinal Plants. Current Research in Bacteriology. 2008; 1 (1):1–6. doi.10.3923/crb.2008.1.6

  • Parekh J. Chanda SV. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology. 2007; 31:53-58.http://dergipark.ord.tr

  • Teke GN. Lunga PK. Wabo HK. Kuiate JR. Vilarem G. Giacinti G, et al. Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie. BMC Complementary Medicine and therapies. 2011;11(1):57. https://doi.org/10.1186/1472-6882-11-57

  • Rai MK. Deepak A. Wadegaonkar P. (2003). Plant derived-antimycotics: Potential of Asteraceous plants, In: Plant-derived antimycotics:Current Trends and Future prospects, Haworth press, N-York, Londin, Oxford, pp 165-185.

  • Iniaghe OM, Malomo SO. Adebayo JO. Proximate composition and phytochemical constituents of leaves of some Acalypha species. Pakistan Journal of Nutrition, 2009:8(3):256-258.doi:10.3923/pjn.2009.256.258

  • Lee HS. Growth-Inhibitory Effects of Various Medicinal Plants against Lactic Bacteria and Harmful Intestinal Bacteria. Food Sci Biotechnol. 2000;9(1):52–6.

  • Hill AF. A textbook of useful plants and plant products, Economic Botany. 2nd ed. New York: Mc Graw-Hill Book company lnc; 1952.

  • Block G. The Data Support a Role for Antioxidants in Reducing Cancer Risk. Nutrition Reviews 2009 Apr 27;50(7):207–13.doi/10.1111/j.1753-4887.1992.tb01329.x

  • Diplock A. Antioxidant nutrients-efficiency in disease prevention and safety. Biochemistry. 1995;34(17):16–8.

  • Jang S. Marchal V. Panigrahi KCS. Wenkel S. Soppe W. Deng XW et al Arabidopsis COP1 shapes the temporal pattern of CO accumulation conferring a photoperiodic flowering response. EMBO J. 2008; 27(8):1277–88. DOI: 10.1038/emboj.2008.68

  • Akihisa T. Yasukawa K.Oinuma H. Kasahara Y. Yamanouchi S. Takido M. et al Triterpene alcohols from the flowers of compositae and their anti-inflammatory effects. Phytochemistry. 1996 ;43(6):1255–60. doi: 10.1016/s0031-9422(96)00343-3.

  • Dubey S. Kumar Singh V. Population Dynamics of Aphis Spiraecola Patch (Homoptera: Aphididae) on Medicinal Plant Cosmos Bipinnatus in Eastern Uttar Pradesh, India. Advances in Life Sciences. 2012; 1(2):54–58. doi: 10.5923/j.als.20110102.10

  • Botsaris AS. Plants used traditionally to treat malaria in Brazil: the archives of Flora Medicinal. Journal of Ethnobiology Ethnomedicine. 2007;3(1):18. https://doi.org/10.1186/1746-4269-3-18

  • Trease GE EW. Pharmacognosy. 15th ed. London: Saunders Publishers; 2002. 42–44. 221–229, 246–249, 304–306, 331–332, 391–393 p.

  • McDonald S. Prenzler PD. Antolovich M. Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chemistry. 2001;73(1):73–84. https://doi.org/10.1016/S0308-8146(00)00288-0

  • Sumitra C. Raj D. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal Microbiology Research. 2009; 3(13):981-996.

  • Ibrahim G. Abdulmumin S. Musa KY YA. Anticonvulsant activities of crude flavonoid fraction of the stem bark of Ficus sycomorus (Moraceae). Journal of Pharmacology and Toxicology. 2008;3(5):351-356. doi: 10.3923/jpt.2008.351.356

  • Benzie IFF. Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Anal Biochem .1996;239(1):70–6.doi:10.1006/abio.1996.0292

  • Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 1958;181(4617):1199–1200. https://doi.org/10.1038/1811199a0

  • Doern G V. Brown SD. Antimicrobial susceptibility among community-acquired respiratory tract pathogens in the USA: Data from PROTEKT US 2000-01. Journal of Infection. 2004;48(1):56-65. doi: 10.1016/s0163-4453(03)00123-3.

  • Sowmya S. Perumal PC. Anusooriya P. Vidya B. Pratibha P.Malarvizhi D. et al Comparative preliminary phytochemical analysis various different parts (stem, leaf and fruit) of Cayratia trifolia (L.). Indo American Journal of Pharm Research.2015;5(01). 218-223.

  • Sengul M. Yildiz H. Gungor N. Cetin B. Eser Z. Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences. 2009 ;22(1):102–106. PMID:19168430

  • Sen S. De B. Devanna N. Chakraborty R. Total phenolic, total flavonoid content, and antioxidant capacity of the leaves of Meyna spinosa Roxb., an Indian medicinal plant. Chinese Journal of Natural Medicine. 2013;11(2):149-157. doi: 10.1016/S1875-5364(13)60042-4.

  • Rangasamy P. Hansiya V. Maheswari P. Thamburaj S. Geetha N. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. Asian Journal of . Pharmeutical Analysis. 2019; 9(2):67-76. doi- 10.5958/2231-5675.2019.00014.0.

  • Hemalatha M. Arirudran B. Thenmozhi A. Mahadeva Rao US. Antimicrobial Effect of Separate Extract of Acetone, Ethyl Acetate, Methanol and Aqueous from Leaf of Milkweed (Calotropis gigantea L.). Asian Journal of Pharmaceutical Research. 2011; 1(4):102-107.

  • Potbhare M. Khobragade D. In Vitro Evaluation of Antioxidant Potential of Ayurvedic Preparations Lauha Bhasma and Mandura Bhasma. Asian Journal of Pharmaceutical Research 2017; 7(2):63-66. doi: 10.5958/2231-5691.2017.00011.9.

  • Ilahi I. Samar S. Khan I AI. In vitro antioxidant activities of four medicinal plants on the basis of DPPH free radical scavenging. Pakistan Journal of Pharmaceutical Sciences. 2013;26(5):949–952.PMID:24035951

  • Valli G. Jeyalakshmi M. Preliminary Phytochemical and Antioxidant Study of Odina woodier Leaf Extract. Asian Journal of Pharmeutical Research. 2012; 2(4): 153-155.

  • Shefali S. Rupali C. Rajan R. Nitin S. Anuradha S. Kamal D. Vikas K. Effect of solvent on yield, phytochemicals and in vitro antioxidant potential of Rhododendron arboreum. Research Journal of Pharmacy and Technology. 2021; 14(1):311-316. doi: 10.5958/0974360X.2021.00057.3

  • Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of ethnopharmacology. 2001 Feb 1;74(2):113-23. doi: 10.1016/s0378-8741(00)00335-4.

  • Suhas SA. Santosh KS. Kiran AW. In vitro Antioxidant potential and Anticancer activity of Ceratophyllum demersum Linn. extracts on HT-29 human colon cancer cell line. Research Journal of Pharmacy and Technology. 2021; 14(1):28-36. doi: 10.5958/0974-360X.2021.00006.8

  • Latifa Nasser AA.Syed Atheruddin Q. Evaluation of Antibacterial and Antioxidant activities of Tribulus terrestris L. Fruits. Research Journal of Pharmacy and Technology. 2021; 14(1):331-336. doi: 10.5958/0974-360X.2021.00061.5

  • Shaheda N. Bhagya Lakshmi L. In-vitro Analysis of Phytochemical, Anti-oxidant capacity of seed Ethanolic extracts of Sapindus saponaria Vahl and Anti-bacterial activity on Common Dental Pathogens. Research Journal of Pharmacy and Technology. 2021; 14(1):351-355. doi: 10.5958/0974-360X.2021.00064.0

  • Deepthi Y. Sivakkumar T. Srinivas N. Phytochemical Evaluation and in-vitro Antioxidant Potential of Whole Plant of Hyptis suaveolens. Research Journal of Pharmacy and Technology. 2021; 14(1):409-412. doi: 10.5958/0974-360X.2021.00074.3

  • Vrutti P. Ravi AM. Devang BS. Evaluation of in-vitro Thrombolytic activity of methanolic extract of Prunus avium L. Asian Journal of Research in Pharmaceutical Sciences. 2021; 11(1):41-44. doi: 10.5958/2231-5659.2021.00007.2


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  • Evaluation of Anti-oxidant, Anti-microbial and Phytochemical Analysis of Different Parts of C. Bipinatus

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Authors

Tejaswi Jasti
Department of Biotechnology, GIT, GITAM Deemed to be University, Visakhapatnam, India, 530045., India
Manisha Senapathi
Department of Biotechnology, GIT, GITAM Deemed to be University, Visakhapatnam, India, 530045., India
Sasank p
Department of Biotechnology, GIT, GITAM Deemed to be University, Visakhapatnam, India, 530045., India
Varaprasad Bobbarala
Department of Biotechnology, GIT, GITAM Deemed to be University, Visakhapatnam, India, 530045., India
Kandra Prameela
Assistant Professor, Department of Biotechnology, GITAM Institute of Technology, GITAM University. Visakhapatnam,, India

Abstract


Plants are sources of many natural active compounds used to prevent several diseases. The various parts of plants like leaf, stem, flower and root are rich of these bioactive compounds. From ancient times these natural compounds have been using for treatment and prevention of diseases. Cosmos bipinnatus is an herbaceous, medicinal plant. The present study was aimed with the objective to antioxidant, antimicrobial and phytochemical screening, total phenolic and total flavonoid content of different solvent extracts of different parts of Cosmos bipinnatus. Flavonoids were abundantly found in the leaves and the root of Cosmos bipinnatus. Steroids were absent only in the leaves and flowers of Cosmos bipinnatus while terpenoids were completely absent in the flowers of the plant. Alkaloids were absent in the leaves extracted from all the three solvents and root sample extracted using ethyl acetate and stem sample extracted using ethanol. Proteins are absent in all the parts of the plant extracted using all the three solvents namely ethanol, ethyl acetate and hexane. Tannins are absent in the entirely in the flower in addition to the stem sample taken using the solvent hexane. Saponins are present all the parts like leaf, stem and flower but are not found in the root extract of the plant. The total phenolic and flavonoid contents of the extracts were calculated by using the standard gallic acid and quercetin graphs to find out the concentration. The total phenolic content is high for the plant sample stem that is extracted by using hexane as the solvent the value is 790μg/mg. The total flavonoid content is high for the plant sample stem that is extracted by using hexane as the solvent the value is 150μg/mg. The antioxidant activity was also found out by using various assays like FRAP and DPPH where the percentage of inhibition was found out. The highest percentage of inhibition for leaf was found out to be at 120μg/ml with the solvent hexane that is 88.86%. The highest percentage of inhibition for stem was found out to be at 60mg/ml using ethanol that is 89.92%. The highest percentage of inhibition for flower was found out to be at 60mg/ml by ethyl acetate that is 91.42%. The highest percentage of inhibition for root was found out to be at 60mg/ml that is 97%. From the FRAP results it is evident that the antioxidant activity is highest for the root sample.

Keywords


Phytochemical analysis, Phenolic content, flavonoid, antioxidant, antimicrobial activity.

References