Research Journal of Pharmacy and Technology

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New Approach for Determination of Phenylephrine Hcl in Pure and Pharmaceutical Formulation Using a Various Microextraction Methods


Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, University of Anbar, Ramadi, Iraq
2 Department of Chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq
3 Department of Chemistry, College of Science, University of Anbar, Ramadi,, Iraq
     

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In this study, ion pair reactions used for evaluation of phenylephrine HCl the development of simple, accurate, low-cost, and fast extraction methods for the separation of phenylephrine HCl was described. The first method was used was the direct extraction that included the reaction PHE with Alizarin yellow reagent to produce yellow complex in the acidic medium (pH=4). The absorbance of this complex was 430nm. The concentration range (1-20μg.mL-1), the Beers law was obeyed with correlation coefficient (R2=0.997), limit of detection as (0.34μg.mL-1), limit of quantification as (1.12μg.mL-1) and molar absorptivity as (14459.9). The second method was dispersive liquid liquid microextraction (DLLME). This method was used to estimation PHE complex by utilizing UV-Vis spectrophotometer. The linearity of calibration curve above was the range between (0.5-13 μg.mL-1), the correlation coefficient (R2=0.996) and molar absorptivity was (12423.3), the detection limit (LOD) and quantification limit (LOQ) were (0.094μg.mL-1) and (0.31μg.mL-1), respectively. This process was successfully method to detect PHE in both pure and pharmaceutical formulations.

Keywords

PhenylephrineHCl (PHE), Direct extraction, DLLME, Spectrophotometer, Pharmaceutical formulation.
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  • New Approach for Determination of Phenylephrine Hcl in Pure and Pharmaceutical Formulation Using a Various Microextraction Methods

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Authors

Nagham K. Kareem
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Anbar, Ramadi, Iraq
Mohammed Z. Thani
Department of Chemistry, College of Science, University of Mustansiriyah, Baghdad, Iraq
Khalid F.Al-Rawi
Department of Chemistry, College of Science, University of Anbar, Ramadi,, Iraq

Abstract


In this study, ion pair reactions used for evaluation of phenylephrine HCl the development of simple, accurate, low-cost, and fast extraction methods for the separation of phenylephrine HCl was described. The first method was used was the direct extraction that included the reaction PHE with Alizarin yellow reagent to produce yellow complex in the acidic medium (pH=4). The absorbance of this complex was 430nm. The concentration range (1-20μg.mL-1), the Beers law was obeyed with correlation coefficient (R2=0.997), limit of detection as (0.34μg.mL-1), limit of quantification as (1.12μg.mL-1) and molar absorptivity as (14459.9). The second method was dispersive liquid liquid microextraction (DLLME). This method was used to estimation PHE complex by utilizing UV-Vis spectrophotometer. The linearity of calibration curve above was the range between (0.5-13 μg.mL-1), the correlation coefficient (R2=0.996) and molar absorptivity was (12423.3), the detection limit (LOD) and quantification limit (LOQ) were (0.094μg.mL-1) and (0.31μg.mL-1), respectively. This process was successfully method to detect PHE in both pure and pharmaceutical formulations.

Keywords


PhenylephrineHCl (PHE), Direct extraction, DLLME, Spectrophotometer, Pharmaceutical formulation.

References