Research Journal of Pharmacy and Technology

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Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass Spectroscopy (Lc-ms)


Affiliations
1 K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
2 Research Scholar, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh-522 502., India
     

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Objective: Highly responsive simple Liquid-liquid Extraction Glipizide development using deuterated Glipizide and Sitagliptin LC-MS/MS Glipizide for gradation of Glipizide and Sitagliptin in rat plasma Glipizide: The chromatographic condition involves, isocratic mode using waters X-Bridge C18 3.5μ (150x4.6mm) column. Mobile phase 0.1% orthophosphoric acid and Acetonitrile in the ratio (80:20) was used and detection was carried out in a positive mode of electrospray ionization by using MS. Results: Glipizide has been validated, the linearity was observed in the range 10-150 ng/ml and 0.1-1.5 ng/ml for Glipizide and Sitagliptin respectively. For intraday and inter-day precision %RSD values were found to be within the acceptable limits. Recovery studies for Glipizide and Sitagliptin obtained was 99.5% and 98.6% respectively. The battery of stability studies like bench-top, Autosampler stability, freeze-thaw, and long term stability was performed. Conclusion: Highly responsive simple liquid chromatography tandem mass spectroscopy assay Glipizidewas developed and witnessed for the gradation of Glipizideand Sitagliptin in rat plasma, the developed Glipizidewas applied to pharmacokinetic studies.

Keywords

LCMS, Glipizide, Sitagliptin, Glipizidevalidation, pharmacokinetic study.
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  • Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass Spectroscopy (Lc-ms)

Abstract Views: 257  |  PDF Views: 0

Authors

A. Anka Rao
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
K. Pavan Kumar
Research Scholar, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh-522 502., India
A. Narayana Rao
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
Narender Malothu
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
B. Prassana Kumar Desu
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
Naga Raju Bandaru
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India

Abstract


Objective: Highly responsive simple Liquid-liquid Extraction Glipizide development using deuterated Glipizide and Sitagliptin LC-MS/MS Glipizide for gradation of Glipizide and Sitagliptin in rat plasma Glipizide: The chromatographic condition involves, isocratic mode using waters X-Bridge C18 3.5μ (150x4.6mm) column. Mobile phase 0.1% orthophosphoric acid and Acetonitrile in the ratio (80:20) was used and detection was carried out in a positive mode of electrospray ionization by using MS. Results: Glipizide has been validated, the linearity was observed in the range 10-150 ng/ml and 0.1-1.5 ng/ml for Glipizide and Sitagliptin respectively. For intraday and inter-day precision %RSD values were found to be within the acceptable limits. Recovery studies for Glipizide and Sitagliptin obtained was 99.5% and 98.6% respectively. The battery of stability studies like bench-top, Autosampler stability, freeze-thaw, and long term stability was performed. Conclusion: Highly responsive simple liquid chromatography tandem mass spectroscopy assay Glipizidewas developed and witnessed for the gradation of Glipizideand Sitagliptin in rat plasma, the developed Glipizidewas applied to pharmacokinetic studies.

Keywords


LCMS, Glipizide, Sitagliptin, Glipizidevalidation, pharmacokinetic study.

References