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Phytochemistry and Cytotoxicity Analysis of Kemang (Mangifera Kemanga) Fruit Extract on Hela Cervical Cancer Cell Line


Affiliations
1 Medical Student, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia
2 Research Center for Biotechnology, Indonesian Institute of Science, Bogor, Indonesia
3 Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia
     

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Mango extract has been proven in its anticancer effect against cervical cancer, however kemang (Mangifera kemanga), despite being a relative of mango, has not been thoroughly researched although expected to give the same effect. Objective: To identify the contents contained in the ethanol, ethyl acetate, and n-hexane extract of kemang flesh and examine its cytotoxic effect on HeLa cervical cancer cells. Methods: Kemang flesh was extracted using ethanol, ethyl acetate, and n-hexane. Phytochemical tests and thin-layer chromatography on the extracts were conducted to identify the phytochemical contents. MTT assay was carried out using the extracts against HeLa cells to find out the cytotoxic effect of the samples in IC50 values. Results: Phytochemical tests revealed that kemang flesh extract contains flavonoid, tannin, triterpenoid, and alkaloid. Thin-layer chromatography test with nonpolar eluent showed one spot with Rf of 0.82 in ethanol extract; six spots with Rf of 0.16, 0.36, 0.49, 0.76, 0.82, and 0.94 in ethyl acetate extract; and six spots with Rf of 0.36, 0.48, 0.63, 0.75, 0.83, and 0.93 in n-hexane extract. MTT assay obtained IC50 values for HeLa cells, respectively for ethanol, ethyl acetate, and n-hexane extracts, were 44.34, 16.41, and 43.23ppm. Conclusion: Kemang fruit extract has potential as an anticancer agent against cervical cancer.

Keywords

HeLa, In-vitro Test, Kemang (Mangifera kemanga) Fruit Extract, Phytochemical test.
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  • Phytochemistry and Cytotoxicity Analysis of Kemang (Mangifera Kemanga) Fruit Extract on Hela Cervical Cancer Cell Line

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Authors

Prajnadiyan Catrawardhana
Medical Student, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia
Ersal Rasyid Saharso
Medical Student, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia
Yusuf Mushlih
Medical Student, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia
Yatri Hapsari
Research Center for Biotechnology, Indonesian Institute of Science, Bogor, Indonesia
Fadilah Fadilah
Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta,, Indonesia

Abstract


Mango extract has been proven in its anticancer effect against cervical cancer, however kemang (Mangifera kemanga), despite being a relative of mango, has not been thoroughly researched although expected to give the same effect. Objective: To identify the contents contained in the ethanol, ethyl acetate, and n-hexane extract of kemang flesh and examine its cytotoxic effect on HeLa cervical cancer cells. Methods: Kemang flesh was extracted using ethanol, ethyl acetate, and n-hexane. Phytochemical tests and thin-layer chromatography on the extracts were conducted to identify the phytochemical contents. MTT assay was carried out using the extracts against HeLa cells to find out the cytotoxic effect of the samples in IC50 values. Results: Phytochemical tests revealed that kemang flesh extract contains flavonoid, tannin, triterpenoid, and alkaloid. Thin-layer chromatography test with nonpolar eluent showed one spot with Rf of 0.82 in ethanol extract; six spots with Rf of 0.16, 0.36, 0.49, 0.76, 0.82, and 0.94 in ethyl acetate extract; and six spots with Rf of 0.36, 0.48, 0.63, 0.75, 0.83, and 0.93 in n-hexane extract. MTT assay obtained IC50 values for HeLa cells, respectively for ethanol, ethyl acetate, and n-hexane extracts, were 44.34, 16.41, and 43.23ppm. Conclusion: Kemang fruit extract has potential as an anticancer agent against cervical cancer.

Keywords


HeLa, In-vitro Test, Kemang (Mangifera kemanga) Fruit Extract, Phytochemical test.

References