Research Journal of Pharmacy and Technology

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Chemical Composition and Evaluation of the Antibacterial and Antifungal Activities of Pre-rif Teucrium Polium Essential Oil


Affiliations
1 Mohammed V University in Rabat, Higher School of Technology of Salé, Material, Energetic and Acoustics Team, Salé,, Morocco
2 Forest Research Center, Rabat,, Morocco
3 Ibn Tofail University of Kenitra, Faculty of Sciences, Laboratory of Agrophysiology, Biotechnology, Environment and Quality.
4 Ibn Tofail University of Kenitra, Faculty of Sciences, Laboratory of Agrophysiology, Biotechnology, Environment and Quality., Morocco
     

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As part of the valorization of Aromatic and Medicinal Plants (AMP) in Morocco, we studied the chemical composition and the antibacterial and antifungal activities of Teucrium polium Essential Oil harvested in a region of Pre-Rif, Taza in Morocco (BniKrama). After extraction of the essential oil (EO) by hydro-distillation process, the study of the chemical composition of this EO was carried out by gas chromatography (GPC) and GC/MS (gas chromatography- mass spectrometry), and the evaluation of antibacterial and antifungal activity was carried out against eleven microbial strains by determining the minimum concentration inhibitor. The yield after extraction by hydro-distillation is 0.71%. After (CPG) of the essence of this plant, sixty-eight components have been identified, of which α-Pinene is the major component (21.96%). It is followed by Limonene (18.77%), and β-Pinene (8.46%). The essential oil of T. polium (EOTP) has shown strong antibacterial and antifungal activity. This bioactivity is mainly due to the richness of this essence in terpenes known by their effectiveness against microbial agents. This essential oil is very rich in chemical molecules. Antimicrobial tests show that this EOTP has a broad spectrum on molds, fungi and bacteria.

Keywords

Antibacterial activity; Antifungal Activity; Aromatic and Medicinal Plant (AMP); Gas Chromatography (GC); Essential oil (EO); Teucrium Polium.
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  • W.H.O. A Global Health Guardian: Climate Change, Air Pollution and Antimicrobial Resistance. World Health Organization 2017.

  • Swapnali M. Rutuja S. Naziya P. Antimicrobial Activity of Leaves extracts of Cassia tora. Res. J. Pharma. Dosage Forms and Tech 2018; 10(1): 10-12.

  • Mariyappan M. Bharathidasan R. Mahalingam R. Madhanraj P. Panneerselvam A. Ambikapathy V. Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla. Asian J. Pharm. Res 2011; 1(4):111-113.

  • W.H.O. Rapport sur la médecine traditionnelle : Besoin et potentiel. World Health Organization 2003.

  • Thilagavathi T. and Kathiravan G. Phytochemical Analysis and Antimicrobial Activity of Ethonolic Leaf Extract of Ficus racemosa Linn. Research J. Pharm. and Tech 2017; 10(2): 537-540.

  • Shakeera Banu M. and Sujatha K. Antimicrobial potential of ethanolic extract of Psidium guajava leaf and its isolated fraction against some pathogenic microorganisms. Research J. Pharmacology and Pharmacodynamics 2012; 4(3):169-171.

  • Jaiganesh KP. Sreedharren B. Arunachalam G. Nirmala R. Nepolean R. Pharmacognostical and Antimicrobial investigation of Jatropha curcas, Linn., Leaf. Asian J. Res. Pharm. Sci 2013; 3(4):195-199.

  • Ramakrishnan R. Samydurai P. Thangapandian V. Qualitative Phytochemical Analysis and In Vitro Antibacterial activity of Glossocardia bosvallia (Linn. f.) Dc. - An Important Ayurvedic Medicinal Herb. Research J. Pharm. and Tech 2013; 6(12):1391-1396.

  • Ayşe B. Betül D. Mehmet YP. Selen İ. Müberra K. Kemal HCB. Fatih D. Chemical Composition of the Essential Oil and Antimicrobial Activity of Scaligeria DC. Taxa and Implications for Taxonomy. Rec. Nat. Prod 2018; 12(1):14-28.

  • Athar A. Sabiha S. Malik MA. Sayeed A. Hifzul K. Abdin MZ. Development of standard operating procedure and standardization of Habb-e-Banafsha Qawi-A Unani polyherbal formulation. J of Pharmacy and Bioallied Sciences 2015; 7(4):250-53.

  • Sheela Rani T. Gopal V. Seethalakshmi S. Chitra K. Quantification and Screening of Phytoconstituents in Selected Medicinal Plant. Research J. Pharm. and Tech 2013; 6(11):1235-1236.

  • Aravind R. Bindu AR. Bindu K. Alexeyena V. GC-MS Analysis of the Bark Essential Oil of Cinnamomum malabatrum (Burman. F) Blume. Research J. Pharm. and Tech 2014; 7(7):754-759.

  • Hamilton A. Medicinal plants and conservation: issues and approaches. Surrey (Royaume Uni): Internatonal plants conservation Unit. 2003.

  • Labiad H. Aljaiyash A. Ghanmi M. Satrani B. Et-tahir A. Aouane M. Fadli M. Chaouch A. Exploring the provenance effect on Chemical composition and Pharmacological bioactivity of the Moroccan essential oils of Laurus nobilis. Research J. Pharm. and Tech 2020; 13(9):4067-4076.

  • Benabid A. Flore et écosystèmes du Maroc. Evaluation et préservation de la biodiversité. Edition Ibis press, Paris, France. 2000; pp. 359.

  • Abdollahi M. Karimpour H. Monsef-Esfehani H. Antinociceptive effects of Teucrium polium L. total extract and essential oil in mouse writhing test. Pharmacol. Res 2003; 48(1):31-35.

  • Khleifat K. Shakhanbeh J. Tarawneh KA. The chronice ffects of Teucrium polium on some blood parameters and histopathology of liver and kidney in the rat. Turkish journal of Biology 2002; 262: 65-71.

  • Henchiri H. Bodo B. Deville A. Dubost L. Zourgui L. Raies A. Grellier P. Mambu L. Sesquiterpenoids from Teucrium ramosissimum. Phytochemistry 2009; 70(11-12):1435-41.

  • Menichini F. Conforti F. Rigano D. Formisano C. Piozzi F. Senatore F. Phytochemical composition, anti-inflammatory and antitumour activities of four Teucrium essential oils from Greece. Food Chem 2009; 115(2):679–686.

  • Djabou N. Lorenzi V. Guinoiseau E. Andreani S. Giuliani MC. Desjobert JM. Bolla JM. Costa J. Berti L. Luciani A. Muselli A. Phytochemical composition of Corsican Teucrium essential oils and antibacterial activity against foodborne or toxi-infectious pathogens. Food Control 2013; 30(1):354-363.

  • Clevenger JF. Apparatus for the determination of volatile oil. J. Am. Pharm. Assoc 1928; 17 :341-346.

  • Kovats E. Gas chromatographic characterization of organic substances in the retention index system. Advances in Chromatography 1965; 229-247.

  • Remmal A. Tantaoui-Elaraki A. Bouchikhi T. Improved method for the determination of microbial activity of essential oils in Agar Medium. J Essent Oil Res 1993; 5(2):179–84.

  • Satrani B. Farah A. Fechtal M. Talbi M. Blaghen M. Chaouch A. Composition chimique et activité antimicrobienne des huiles essentielles de Satureja calamintha et Satureja alpina du Maroc. Ann. Fals. Exp. Chim. Toxic 2001; 956:241-250.

  • Eikani MH. Goodarznia I. Mirza M. Comparison between the essential oil and supercritical carbon dioxide extract of Teucrium polium L. J. Essent. Oil Res 1999; 11:470–472.

  • Belmekki N. Bendimerad N. Bekhechi C. Fernandez X. Chemical analysis and antimicrobial activity of Teucrium polium L. essential oil from Western Algeria. Journal of Medicinal Plants Research 2013; (714) :897-902.

  • Bendif H. Caractérisation phytochimique et détermination des activités biologiques in vitro des extraits actifs de quelques Lamiaceae. Phd thesis, the Kouba-Alger University, Alger 2017.

  • Fertout-Mouri N. Latrèche A. Mehdadi Z. Toumi-Bénali F. Khaled MB. Composition chimique et activité antibactérienne de l’huile essentielle de Teucrium polium L. du mont de Tessala (Algérie occidentale). Phytothérapie 2017; 15(6): 346-353.

  • El Atki Y. Aouam I. El Kamari F. Taroq A. Lyoussi B. Oumokhtar B. Abdellaoui A. Phytochemistry, antioxidant and antibacteriala ctivities of two Moroccan Teucrium polium L. subspecies: Preventive approach against nosocomial infections. Arabian Journal of Chemistry 2019; 13(2): 3866-3874.

  • Cozzani S. Muselli A. Desjobert JM. Bernardini AF. Tomi F. Casanova J. Chemical composition of essential oil of Teucrium polium subsp. capitatum (L.) from Corsica. Flavour and Fragrance Journal 2005; 20(4):436-441.

  • Bencheikh SE. Segni L. Goudjil MB. Mehani M. Zighmi S. Chemical analysis, antimicrobial and antioxidant activity of the essential oil of Teucrium polium ssp aurasianum Labiatae. Der Pharma Chemica 2015; 7(12):308-314.

  • Satrani B. Ghanmi M. Farah A. Aafi A. Fougrach H. Bourkhiss B. Bousta D. Talbi M. Composition chimique et activité antimicrobienne de l’huile essentielle de cladanthus mixtus. Bull. Soc. Pharm. Bordeaux 2007; 146:85-96.

  • Ghanmi M. Satrani B. Aafi A. Isamili MR. Houti H. El Monfalouti H. Benchakroun HK. Harki L. Boukir A. Chaouch A. Charrouf Z. Effet de la date de récolte sur le rendement, la composition chimique et la bioactivité des huiles essentielles de l’armoise blanche (Artemisia herba-alba) de la région de Guerçif (Maroc oriental). Phytotérapie 2010; 8(5):295-301.

  • Moualla N. Naser M. Using GC/MS to Study the Chemical Composition of Essential Oil of Thymus vulgaris L. at AL-Qadmous Area, Syria. Research J. Pharm. and Tech 2015; 8(4):437-442.

  • Shruthi C. Geetha RV. Antibacterial Activity of the Three Essential Oils on Oral Pathogens- An In-vitro Study. Research J. Pharm. and Tech 2014; 7(10):1128-1129.


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  • Chemical Composition and Evaluation of the Antibacterial and Antifungal Activities of Pre-rif Teucrium Polium Essential Oil

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Authors

Oussama Chauiyakh
Mohammed V University in Rabat, Higher School of Technology of Salé, Material, Energetic and Acoustics Team, Salé,, Morocco
Aziz Et-Tahir
Mohammed V University in Rabat, Higher School of Technology of Salé, Material, Energetic and Acoustics Team, Salé,, Morocco
Badr Satrani
Forest Research Center, Rabat,, Morocco
Mohamed Ghanmi
Forest Research Center, Rabat,, Morocco
Sara Cherrad
Ibn Tofail University of Kenitra, Faculty of Sciences, Laboratory of Agrophysiology, Biotechnology, Environment and Quality.
Kamal Kettani
Mohammed V University in Rabat, Higher School of Technology of Salé, Material, Energetic and Acoustics Team, Salé,, Morocco
Abdelaziz Chaouch
Ibn Tofail University of Kenitra, Faculty of Sciences, Laboratory of Agrophysiology, Biotechnology, Environment and Quality., Morocco
Malika Tiskar
Ibn Tofail University of Kenitra, Faculty of Sciences, Laboratory of Agrophysiology, Biotechnology, Environment and Quality., Morocco

Abstract


As part of the valorization of Aromatic and Medicinal Plants (AMP) in Morocco, we studied the chemical composition and the antibacterial and antifungal activities of Teucrium polium Essential Oil harvested in a region of Pre-Rif, Taza in Morocco (BniKrama). After extraction of the essential oil (EO) by hydro-distillation process, the study of the chemical composition of this EO was carried out by gas chromatography (GPC) and GC/MS (gas chromatography- mass spectrometry), and the evaluation of antibacterial and antifungal activity was carried out against eleven microbial strains by determining the minimum concentration inhibitor. The yield after extraction by hydro-distillation is 0.71%. After (CPG) of the essence of this plant, sixty-eight components have been identified, of which α-Pinene is the major component (21.96%). It is followed by Limonene (18.77%), and β-Pinene (8.46%). The essential oil of T. polium (EOTP) has shown strong antibacterial and antifungal activity. This bioactivity is mainly due to the richness of this essence in terpenes known by their effectiveness against microbial agents. This essential oil is very rich in chemical molecules. Antimicrobial tests show that this EOTP has a broad spectrum on molds, fungi and bacteria.

Keywords


Antibacterial activity; Antifungal Activity; Aromatic and Medicinal Plant (AMP); Gas Chromatography (GC); Essential oil (EO); Teucrium Polium.

References