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Analysis of Phytochemical Compounds Present in Some Traditional Anti-dermatophytic Plants by Thin Layer, Column Chromatographic Methods


Affiliations
1 PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
2 School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia
     

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Traditionally, various medicinal plants were known to possess anti-dermatophytic potential and have been used for many years to treat skin diseases. We are aimed to examine anti-dermatophytic plants like Calotropis gigantea (L.) R. Br., Cassia alata (L) Roxb., Kaempferia galanga L., Melia azedarach L. and Sesbania grandiflora (L) Pers. for the quantification and separation of phyto-chemical compounds through thin layer and column chromatography. Five plant samples comprising of leaves, stem fiber and rhizome were separately extracted using five solvents such as, ethanol, methanol, acetone, chloroform and petroleum ether. The solvent extracts were subsequently investigated for qualitative, quantitative phytochemical, thin layer and column chromatographic analysis using standard assays. The experimental findings revealed the varied presence of compounds such as sugar, amino acid, protein, phenol, alkaloid and flavonoid in the solvent extracts used. In TLC profiling, a number of phyto chemical compounds, at different ranges of visible spots, with different Rf values at varying solvent system, were identified. Also, column fractions at different solvent run ratio (hexane: acetone = 8:2, 6:4, 4:6, 2:8) were identified with a wide range of color variations at different retention time and were collected sequentially. The present analysis exhibit a significant role to identify and differentiate therapeutic phyto-compounds which helps for further study on isolation and characterization with anti-dermatophytic potential.

Keywords

Anti-dermatophytic, Phytochemical compounds, Thin layer, Column, Chromatography.
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  • Analysis of Phytochemical Compounds Present in Some Traditional Anti-dermatophytic Plants by Thin Layer, Column Chromatographic Methods

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Authors

Sownthariya C
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Shanthi P
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Balavinayagamani Ganapathy
School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia
Priyadharshni K
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Thiripura Sundari U
PG and Research Department of Botany, Holy Cross College (Autonomous), Tiruchirappalli - 620002, Tamil Nadu,, India
Nagaraja Suryadevara
School of Biosciences, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor,, Malaysia

Abstract


Traditionally, various medicinal plants were known to possess anti-dermatophytic potential and have been used for many years to treat skin diseases. We are aimed to examine anti-dermatophytic plants like Calotropis gigantea (L.) R. Br., Cassia alata (L) Roxb., Kaempferia galanga L., Melia azedarach L. and Sesbania grandiflora (L) Pers. for the quantification and separation of phyto-chemical compounds through thin layer and column chromatography. Five plant samples comprising of leaves, stem fiber and rhizome were separately extracted using five solvents such as, ethanol, methanol, acetone, chloroform and petroleum ether. The solvent extracts were subsequently investigated for qualitative, quantitative phytochemical, thin layer and column chromatographic analysis using standard assays. The experimental findings revealed the varied presence of compounds such as sugar, amino acid, protein, phenol, alkaloid and flavonoid in the solvent extracts used. In TLC profiling, a number of phyto chemical compounds, at different ranges of visible spots, with different Rf values at varying solvent system, were identified. Also, column fractions at different solvent run ratio (hexane: acetone = 8:2, 6:4, 4:6, 2:8) were identified with a wide range of color variations at different retention time and were collected sequentially. The present analysis exhibit a significant role to identify and differentiate therapeutic phyto-compounds which helps for further study on isolation and characterization with anti-dermatophytic potential.

Keywords


Anti-dermatophytic, Phytochemical compounds, Thin layer, Column, Chromatography.

References