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Acute Oral Toxicity of Essential Oil Extracted from the Leaves of Ocimum Sanctum L. (Thulasi) in Female Wistar Albino Rats.


Affiliations
1 Professor of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth, Deemed to be University, Puducherry., India
2 Veterinary surgeon, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
3 Associate Professor, Pathology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
4 Professor, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
5 Professor and HOD, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
6 Professor and HOD, Microbiology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
7 Professor, Microbiology department, Mahatma Gandhi Medical College and Research Center., Puducherry., India
     

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Due to the widespread use of antimicrobial agents, many Microorganisms have developed drug resistance to many available antimicrobials today. Essential oils from different plants are being explored for their ability to treat various human diseases, including infectious diseases. Earlier, we have found that the essential oil from the leaves of Ocimum sanctum L. had anti bacterial effect on 18 human pathogenic bacteria. In the present study, the essential oil of Ocimum sanctum L. was evaluated for its toxic effects at 300 mg / Kg body weight and 2000 mg / Kg body weight of female Wistar albino rats as per the OECD Guidelines 423 for testing of chemicals. The 14 day acute oral toxicity test has shown Ocimum sanctum L. essential oil is not toxic at 300 mg / Kg body weight of animals but has some toxic effects at 2000 mg /Kg body weight of animals. However, no animals died during the 14 day acute oral toxicity test with 2000 mg / Kg body weight of animals and therefore the LD50 dose of Ocimum sanctum L. should be more than 2000 mg / Kg of body weight of female Wister albino rats.

Keywords

Ocimum sanctum, Essential oil, Acute oral toxicity, Wistar albino female rats, Thulasi.
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  • Organization for Economic Cooperation and Development. OECD guidelines-423.17 th December 2001.

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  • Acute Oral Toxicity of Essential Oil Extracted from the Leaves of Ocimum Sanctum L. (Thulasi) in Female Wistar Albino Rats.

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Authors

Venugopal Jayapal
Professor of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth, Deemed to be University, Puducherry., India
V. Subha
Veterinary surgeon, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
K. Bhavani
Associate Professor, Pathology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Kartik Janardan Salwe
Professor, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
K. Manimekalai
Professor and HOD, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Josh M Easow
Professor and HOD, Microbiology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Stephen Selvaraj
Professor, Microbiology department, Mahatma Gandhi Medical College and Research Center., Puducherry., India

Abstract


Due to the widespread use of antimicrobial agents, many Microorganisms have developed drug resistance to many available antimicrobials today. Essential oils from different plants are being explored for their ability to treat various human diseases, including infectious diseases. Earlier, we have found that the essential oil from the leaves of Ocimum sanctum L. had anti bacterial effect on 18 human pathogenic bacteria. In the present study, the essential oil of Ocimum sanctum L. was evaluated for its toxic effects at 300 mg / Kg body weight and 2000 mg / Kg body weight of female Wistar albino rats as per the OECD Guidelines 423 for testing of chemicals. The 14 day acute oral toxicity test has shown Ocimum sanctum L. essential oil is not toxic at 300 mg / Kg body weight of animals but has some toxic effects at 2000 mg /Kg body weight of animals. However, no animals died during the 14 day acute oral toxicity test with 2000 mg / Kg body weight of animals and therefore the LD50 dose of Ocimum sanctum L. should be more than 2000 mg / Kg of body weight of female Wister albino rats.

Keywords


Ocimum sanctum, Essential oil, Acute oral toxicity, Wistar albino female rats, Thulasi.

References