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Acute Oral Toxicity of Essential Oil Extracted from the Leaves of Ocimum Sanctum L. (Thulasi) in Female Wistar Albino Rats.


Affiliations
1 Professor of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth, Deemed to be University, Puducherry., India
2 Veterinary surgeon, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
3 Associate Professor, Pathology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
4 Professor, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
5 Professor and HOD, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
6 Professor and HOD, Microbiology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
7 Professor, Microbiology department, Mahatma Gandhi Medical College and Research Center., Puducherry., India
     

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Due to the widespread use of antimicrobial agents, many Microorganisms have developed drug resistance to many available antimicrobials today. Essential oils from different plants are being explored for their ability to treat various human diseases, including infectious diseases. Earlier, we have found that the essential oil from the leaves of Ocimum sanctum L. had anti bacterial effect on 18 human pathogenic bacteria. In the present study, the essential oil of Ocimum sanctum L. was evaluated for its toxic effects at 300 mg / Kg body weight and 2000 mg / Kg body weight of female Wistar albino rats as per the OECD Guidelines 423 for testing of chemicals. The 14 day acute oral toxicity test has shown Ocimum sanctum L. essential oil is not toxic at 300 mg / Kg body weight of animals but has some toxic effects at 2000 mg /Kg body weight of animals. However, no animals died during the 14 day acute oral toxicity test with 2000 mg / Kg body weight of animals and therefore the LD50 dose of Ocimum sanctum L. should be more than 2000 mg / Kg of body weight of female Wister albino rats.

Keywords

Ocimum sanctum, Essential oil, Acute oral toxicity, Wistar albino female rats, Thulasi.
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  • Politeo O et al. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil. Food Chemistry. 2007; 101:379-385.

  • Chil Nunez I et al. Chemical composition and toxicity of Ocimum sanctum L. Var. Cubensis Essential oil up-growing in the eastern of Cuba. International Jl Pharmacognosy and Phytochemical Research. 2017: 9(7): 1021-1028.

  • Singh S et al. Biological activities of Ocimum sanctum L. fixed oil-An overview. Indian J Exp Biol. 2007; 45 (5) : 403-412

  • Brophy JJ et al. The essential oil of Ocimum tenuiflorum L. (Lamiaceae) growing in Northern Australia. J Essen Oil Res.1993; 5 : 459-461.

  • Machado MIL et al. Volatile constituents from leaves and inflorescence oil of Ocimum tenuiflorum L.f ( syn Ocimum sanctum L ) grown in Northeastern Brazil. J Essen Oil Res.1999; 11 : 324-326.

  • Viana A and Murillo E. Essential oil composition from twelve varieties of Basil ( Ocimum spp.) grown in Colombia. J Braz Chem Soc. 2003; 14 : 744-749.

  • Kothari SK et al. Essential oil yield and quality of methyl eugenol rich Ocimum tenuiflorum L.f. ( Syn Ocimum sanctum L.) grown in South India as influenced by method of harvest. J Chromatogr A. 2004; 1054: 67-72.

  • 14.Cannon JB et al. Modification of yield and composition of essential oils by distillation time. Ind Crops Prod.2013 ; 41:214-220.

  • Venugopal Jayapal. Antibacterial effect of essential oil of Ocimum sanctum L. by minimal bactericidal concentration, disc diffusion, and gaseous contact exposure methods over 18 bacteria. Jl Pharmacognosy and Phytochemistry. 2018; 7(5) : 3049-3055.

  • Prasad et al. Antimicrobial activity of essential oils from some Ocimum species and clove oil. Fitoterapia. 1986; 57 : 429-432 11 Nakamura CV et al. Antibacterial activity of Ocimum gratissimum L, essential oil. 1999; Memorias do Instituto Oswaldo Cruz. 1999 ; 94: 675-678

  • Adebolu TT and Oladimeji SA. Antimicrobial activity of leaf extracts of Ocimum gratissimum on selected diarrhea causing bacteria in southwestern Nigeria. African Journal of Biotechnology. 2005 ; 4 :682-684

  • Adiguzel A et al. . Antimicrobial effects of Ocimum basilicum (Labiate) extract. Turkish J of Biology. 2005; 29:155-160.

  • Verma RS et al.. Chemical composition and antibacterial activity of essential oil from two Ocimum spp. grown in sub-tropical India during spring-summer cropping season. Journal of Traditional Medicines. 2011; 6 : 211-217

  • Yamani HA et al.. Antimicrobial activity of Tulsi (Ocimum tenuiflorum) Essential Oil and their major constituents against three species of bacteria. Front Microbiol. 2016 ; 7 :681-692

  • Bhargava KP and Singh N. Anti-stress activity of Ocimum sanctum Linn. Ind J Med Res .1981; 73 :443-451

  • Gautam MK and Goel RK. Toxicological study of Ocimum sanctum Linn leaves: Hematological, Biochemical, and Histopathological studies.J Toxicol. 2014; published online 2014 Jan 29.doi: 10.1155/2014/135654. PMID: 24616736 PMCID: PMC3927567

  • Organization for Economic Cooperation and Development. OECD guidelines-423.17 th December 2001.


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  • Acute Oral Toxicity of Essential Oil Extracted from the Leaves of Ocimum Sanctum L. (Thulasi) in Female Wistar Albino Rats.

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Authors

Venugopal Jayapal
Professor of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth, Deemed to be University, Puducherry., India
V. Subha
Veterinary surgeon, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
K. Bhavani
Associate Professor, Pathology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Kartik Janardan Salwe
Professor, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
K. Manimekalai
Professor and HOD, Pharmacology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Josh M Easow
Professor and HOD, Microbiology Department, Mahatma Gandhi Medical College and Research Institute, Puducherry., India
Stephen Selvaraj
Professor, Microbiology department, Mahatma Gandhi Medical College and Research Center., Puducherry., India

Abstract


Due to the widespread use of antimicrobial agents, many Microorganisms have developed drug resistance to many available antimicrobials today. Essential oils from different plants are being explored for their ability to treat various human diseases, including infectious diseases. Earlier, we have found that the essential oil from the leaves of Ocimum sanctum L. had anti bacterial effect on 18 human pathogenic bacteria. In the present study, the essential oil of Ocimum sanctum L. was evaluated for its toxic effects at 300 mg / Kg body weight and 2000 mg / Kg body weight of female Wistar albino rats as per the OECD Guidelines 423 for testing of chemicals. The 14 day acute oral toxicity test has shown Ocimum sanctum L. essential oil is not toxic at 300 mg / Kg body weight of animals but has some toxic effects at 2000 mg /Kg body weight of animals. However, no animals died during the 14 day acute oral toxicity test with 2000 mg / Kg body weight of animals and therefore the LD50 dose of Ocimum sanctum L. should be more than 2000 mg / Kg of body weight of female Wister albino rats.

Keywords


Ocimum sanctum, Essential oil, Acute oral toxicity, Wistar albino female rats, Thulasi.

References