Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Muhtadi, Ahmad
- Hypoglycemic Activity of Ethyl Acetate Fraction of Mengkudu Fruit (Morinda citrifolia L.) in Mice Induced Alloxan
Abstract Views :152 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacology and Pharmacy Clinic, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, ID
2 Department of Pharmaceutical Biology, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, ID
1 Department of Pharmacology and Pharmacy Clinic, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, ID
2 Department of Pharmaceutical Biology, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 197-201Abstract
Complementary medicine for diabetes mellitus using medicinal plants is the solution chosen as an alternative treatment. Mengkudu fruit (Morinda citrifolia L.) is empirically used by the community for the treatment of diabetes mellitus. To study the hypoglycemic activity of subfraction mengkudu fruit in mice induced alloxan. The test results on subfraction of ethyl acetate fraction (dose 150 mg/kgBW) induced alloxan (dose 175 mg/kgBW) showed that there was a decrease in blood glucose levels including Mc-IIb (33.94%), Mc-IIc (32.21%), Mc-IIa (29.58%), Mc-IId (18.16%) and Mc-IIe (8.09%) were significantly different compared to the negative control (p<0.05). Subfraction Mc-IIb shows that the most active hypoglycemic activity may be due to some secondary metabolites such as flavonoids, saponins, alkaloids, and polyphenols as antidiabetic bioactive principles. These research showed that Mc-IIb subfraction indicates the highest hypoglycemic activity. However, the research for the active hypoglycemic compound of Mc-IIb subfraction needs to be explored further.Keywords
Morinda citrifolia, Ethyl Acetate Fraction, Subfraction, Hypoglycemic, Alloxan.
References
- International Diabetes Federation. Diabetes Atlas 8th edition. Diabetes Atlas. 2017
- Novo Nordisk. Where Economics and Health Meet: Changing Diabetes in Indonesia. In N. Nordisk, The Blueprint for Change Programme Novo Nordisk: Indonesia. 2013; pp. 3.
- Nerurkar, P.V., Phoebe W. Hang and Erik Saksa. Anti-Diabetic Potential of Noni : The Yin and the Yang, Molecules. 2015; 20: 17684-17719.
- Adnyana IK, Elin Yulinah, Andreanus A. Soemardji, Endang Kumolosasi, Maria Immaculata Iwo, Joseph Iskendiarso Sigit, and Suwendar. Antidiabetic Activity of Ethanol Extract Noni Fruit (Morinda citrifolia L.). Acta Pharmaceutica Indonesia. 2004; 29(2): 43-49.
- Rao, U.S.Mahadeva, and Subramanian, S. Biochemical Evaluation of Antihyperglycemic and Antioxidative Effects of Morinda citrifolia Fruit Extract Studied in Streptozotocin-Induced Diabetic Rats. Medical Chemistry Research. 2008; 18, 433-446.
- Kustarini, I., Dewi, S.S., and Ika, P.M. Ethanol Extract of Morinda citrifolia L. (Mengkudu), Blood Glucose, Neutrophyl Count, and Glomerulus Fibronectin in Diabetes Mellitus Rat. Media Medika Indonesiana. 2012; 46(3): 178-183.
- Hadijah, H., Ayub, M.Y., Zaridah, H., and Normah, A. Hypolipidemic Activity of an Aqueous Extract Of Morinda Citrifolia Fruit in Normal and Streptozotocin-Induced Diabetic Rats. Journal of Tropical Agriculture and Food Science. 2008; 36(1): 77-85.
- Nayak, B. Shivananda, Marshall, Julien R., Isitor, Godwin, Adogwa, Andrew. Hypoglycemic and Hepatoprotective Activity of Fermented Fruit Juice of Morinda citrifolia (Noni) in Diabetic Rats. Evidence-Based Complementary and Alternative Medicine. 2011; 1-5.
- Ramdhini, N.S. Antidiabetic Activity of Water Fraction, Ethyl acetate and n-hexane of Noni Fruit (Morinda citrifolia L.) in White Mice. Essay. Faculty of Pharmacy, Padjadjaran University, Indonesia. (2005).
- Farnsworth, N.R. Biological and Phytochemical Screening of Plants. Journal of Pharmaceutical Sciences. 1966; 55(3): 225-276.
- Zhou, Z.I., L. Jing, G. Cui, Q. Feng and Y. Xiao. Effect of polysaccharide from Lycium barbarum in Alloxan-Induced Diabetic Mice. African J. Biotech. 2009; 8(23): 6634-6637.
- Diniz, S.F., Amorim, F.F. Cavalcante-Neto, A.L. Bocca, A.C. Batista, G.E.P.M. Simm and T.A. Silva. Alloxan Induced Diabetes Delays Repair in a Rat Model of Closed Tibial Fracture. Brazilian J. Med. Biol. Res. 2008; 41:373-379.
- Goodman Gilman, A., Hardman, J. G., and Limbird, L. E. (Eds.). Goodman & Gilman’s the Pharmacological Basis of Therapeutics, 10th ed., McGraw-Hill, New York. 2001; pp. 1701.
- Harvey, R.A. and Champe, P.C. Lippincott’s Illustrated Reviews: Pharmacology, Lippincott-Raven, New York, 1992; pp. 260.
- Heinicke RM. The Pharmacologically Active Ingredient of Noni. Pacific Tropical Botanical Garden Bulletin. 1985; 15:10-14.
- Zega VL, Pemsi M. Wowor, Christi Mambo. Test multiple doses of Morinda citrifolia Extract on Blood Glucose Levels in Alloxaninduced Wistar Rats (Rattus norvegicus). Journal e-Biomedik (eBm). 2016; 4(2): 1-5.
- Effect of Pelvic Floor Muscle Training with Biofeedback on Stress Urinary Incontinence in Females, Kelantan, Malaysia: A Pilot Study
Abstract Views :151 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran., IN
2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, IN
3 Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran., IN
4 Faculty Of Pharmacy, Universitas Halu Oleo, Kendari, Southeast Sulawesi, ID
5 Departemen of Pharmacy, Faculty of Science and Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID
6 Departement of Medical Laboratory Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID
1 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran., IN
2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, IN
3 Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran., IN
4 Faculty Of Pharmacy, Universitas Halu Oleo, Kendari, Southeast Sulawesi, ID
5 Departemen of Pharmacy, Faculty of Science and Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID
6 Departement of Medical Laboratory Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID
Source
Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1837-1842Abstract
Introduction: Lawsonia inermis L is a medicinal herb from Lythraceae family. This plant is proven to be able to treat various diseases. Lawsonia inermis L is one of the flowering plant of a single species of the genus Lawsonia. This plant contains the main active compounds such as flavonoids, phenols, alkaloids, glycosides, saponins, tannins, and essential oils. Flavonoids and phenols are the active compounds which most commonly found. Objective : This study aims to determine the quality parameters of the extract which include specific and non-specific parameters, antioxidant activity and total flavonoid content in purified extracts of Lawsonia inermis L. Methodology: The extract was obtained by maceration method using 96% ethanol. Purified extract was obtained by separating the chlorophyll and tannins contained in the sample using ethanol and aqudes, then continued by partitioning using ethyl acetate and adding MgSO4 as a drying agent. The ethyl acetate phase was concentrated using a Rotary Vacuum Evaporator to obtain a purified extract with a reddish black color. Furthermore, the extract was analyzed using thin layer chromatography (TLC) using eluent n-hexane: ethyl acetate (1: 1). Result : Specific parameters include organoleptic observations, water-soluble compounds and ethanol-soluble compounds, while non-specific parameters include drying loss, moisture content, total ash content, acid soluble content, specific gravity, microbial contamination, mold/yeast contamination, Pb metal contamination, Cd and As. The antioxidant activity assay used the DPPH method and the IC50 value was 39.07 mg / L, while the total flavonoid test used quercetin as a comparison and the total flavonoid content was 3.29%. Conclusion : The purified extract of Lawsonia inermis L. leaves comply the quality standard of the extract, has very strong antioxidant activity and contains flavonoids.Keywords
Lawsonia inermis L, Standardization, Radical DPPH, Total Flavonoids.References
- Gagandeep C, Sandeep G, Priyanka P. Lawsonia inermis Linnaeus: A Phytopharmacological Review International Journal of Pharmaceutical Sciences and Drug Research. 2010; 2(2): 91-98.
- Hasiani VV, Ahmad I, Rijai L. Isolasi jamur endofit dan produksi metabolit sekunder antioksidan dari daun pacar (Lawsonia inermis L.). Jurnal Sains dan Kesehatan. 2015;1(4):146-153.
- Chauhan H.V. In-Vivo Antidiabetic, Lipid Lowering and Antioxidant Activities of Methanolic Extaract of Lawsonia inermis Leaves. Research J. Pharm. and Tech. 2011; 4(5):764-767
- Patel KM , Patel PR. Review on Lawsonia inermis Linn. : An Update. Asian J. Pharm. Tech. 2017; 7(4) : 238-250. 5. Das PK, Mondal AK. Studies on traditional ‘mehendi’ used as herbal colour with special references to its antimicrobial activity and pigment profile by TLC. International Journal of Science and Nature. 2012; 3(4):799-804.
- Chaudhary, iqbal, Nizzam Udin, Binna Shaleh, Aslan Huma, Ajmal Khan and Parveen Sahidah. Bioactive flavonoids from the leaves of Lawsonia alba (Henna). Research Institute of Chemistry, International Center For Chemical and Biological Sciences, University of Karachi, Karachi Pakistan. 2011;04(11) 454-458.
- Koirala, N., Pandey, R.P., Parajuli, P., Jung, H.J., Sohng, J.K. Methylation and Subsequent Glycosylation of 7,8-dihydroxyflavone. Journal of Biotechnology. 2014;184, 128–37.
- Cartea, M.E., Francisco, M., Soengas, P., Velasco, P. Phenolic Compounds in Brassica Vegetables, Molecules, 2011; 16: 251-280.
- Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., Kalayci, O. Oxidative Stress and Antioxiant Defense, World Allergy Organization Journal. 2012; pp. 9 – 10, 14.
- Husni E, Suharti N, and Atma A,P,T. 2018. Karakterisasi Simplisia dan Ekstrak Daun Pacar Kuku (Lawsonia inermis Linn) serta Penentuan Kadar Fenolat Total dan Uji Aktivitas Antioksidan. Jurnal Sains Farmasi & Klinis.512-516.
- Madhvi S.K, Sharma M, Iqbal J, Younis M, Sheikh R. Phytochemical analysis, Total Flavonoid, Phenolic contents and Antioxidant activity of extracts from the Leaves of Rhododendron arboretum. Research J. Pharm. and Tech. 2020; 13(4):1701-1706
- Juliawati L.D, Ra’idah P.N, Abdurrahman S, Hermawati E, Alni A,Tan M.I, Ishikawa H and Syah Y.M. 5,6-Dihydro-α-pyrones from the leaves of Cryptocarya pulchinervia (Lauraceae). Journal of Natural Medicines. 2020; (74) :584–590.
- Mohapatra P., Shirwaikara A., Aswatharam HN. Standardization of a Polyherbal Formulation. Pharmacognosy Magazine 2008; 4(13); 65-69.
- Depkes RI. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Dirjen Pengawasan Obat dan Makanan.Volume 1 : Jakarta.
- Shahin K, Ajay K.M, Saluja A.K and Padhi, MM. Pharmacognostical and Physicochemical Evaluation of Anthelmintic an Ayurvedic Polyherbal Formulation: Krumighattini Tablet. Asian J. Research Chem. 2015; 8(11): 697-700.
- Muhammad S, Gul A. K, Akhtar A, Zakir U and Fazl S. Standardization of Eclipta alba (L). Asian J. Research Chem.2011; 4(12):1825-1828.
- Harbone, J.B., 1987. Metode Fitokimia, Penuntun Cara Modern Menganalisa Tumbuhan, Terbitan II. ITB : Bandung.
- Pathak A.V, Kawtikwar P. S, Sakarkar D. M. Pharmacognostical and Physico-Chemical Standardization of Shatavari Churna: An Official Ayurvedic Formulation. Research J. Pharm. and Tech. 2015; 8(11):1495-1501.
- Farnsworth, N.H. Biological and Phytochemical Screening Plant, Pharm Science, 1996; 245-246, doi: https://doi.org/ 10.1002/jps.2600550302
- Gintingg , B, Mustanir, Hira H., Lidya S.D., Era L., Rohmat M., 2017, Antixidant Activity of n- Hexana Extract of Nutmeg Plants from South Aceh Province, Jurnal Natural, Vol.17, No.1, 2017 pISSN 1411-8513, eISSN 2541-4062.
- Narmatha. K, Arul. P, Jeyamurugan S, Srinivasan R, Vignesh. Standardisation of Rhizome Extract of Acorus calamus Linn. Asian J. Research Chem. 2018; 11(2):423-426.
- Thomas S. Patil DA. Patil AG and Chandra N. Pharmacognostic evaluation and physicochemical analysis of Averrhoacarambola L. fruit. J Herb Med Tox 2008; 2(2): 51-54.
- Chang, C. Yang, M. Wen, H. and Chern J. 2002.Estimation of total flavonoid contentin Propolis by two complementary colorimetric methods.J .Food Drug A.
- Qureshi M.S, Reddy A.V, Kumar G.S and Nousheen L. Pharmacognostic, Physicochemical Standardization and PhytochemicalAnalysis of leaves of Hydrolea zeylanica Vahl. (Hydrophyllaceae). Research Journal of Pharmacognosy and Phytochemistry.2017; 9(1): 1-7
- Zou, Y., Lu, Y., Wei, D. 2004. Antioxidant activity o flavonoid-rich extract of hypericum perforatum L in vitro. Journal of Agriculture and food Chemistry 52: 5032-50.