Refine your search
Collections
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Jayasuriya, Banukie
- Determination of Anti-tuberculosis Activity of Psychotria Sarmentosa, Aponogeton Crispus and Two Species of Pleurotus Mushrooms
Abstract Views :98 |
PDF Views:0
Authors
Jayamini Jayantha
1,
Banukie Jayasuriya
2,
Dilanthi Herath
2,
Sugandhika Suresh
3,
Dhammika Magana-Arachchi
4
Affiliations
1 Postgraduate Institute of Science, University of Peradeniya,, LK
2 Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura,, LK
3 Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,, LK
4 Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy,, LK
1 Postgraduate Institute of Science, University of Peradeniya,, LK
2 Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura,, LK
3 Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,, LK
4 Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy,, LK
Source
Research Journal of Pharmacy and Technology, Vol 15, No 3 (2022), Pagination: 954-960Abstract
Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (Mtb) complex. The global TB epidemic has been aggravated by the emergence of disease outbreaks caused by multi-drug resistant and extensively drug-resistant strains. The aim of the present study was to evaluate the in-vitro, anti-TB activity of leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms Pleurotus ostreatus and Pleurotus cystidiosus found in Sri Lanka. Leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms; Pleurotus ostreatus and P. cystidiosus were dried until a constant weight and 120 g each were taken to prepare crude extracts with distilled water (1.9 L) by heating at a moderate temperature and the final volume was reduced to 240 ml. Freeze dried aqueous extracts were incorporated in Middle Brook 7H11 medium (1mg/ml) using pour plate method.Two ten-fold dilutions (10-2 and 10-4) of standard H37Rv Mtb suspensions were inoculated on Middle Brook 7H11 media with the crude extracts. The plates were incubated at 37 0C for 4 weeks until visible appearance of Mtb colonies. The inhibitory effect of each extract was calculated by the mean reduction of number of colonies on extract containing medium compared to extract-free control medium. Accordingly, the highest mean percentage inhibition was shown by P. sarmentosa (71.0 %). The mean percentage inhibition exerted by A. crispus, P. ostreatus and P. cystidiosus were 46.0 %, 43.4 % and 39.5 % respectively. Therefore, freeze-dried aqueous extract from leaves of P. sarmentosa has certain activity against the tested standard mycobacterial strain and has a potential to be used as an anti-TB drug component.Keywords
Psychotria sarmentosa, Aponogeton crispus, Pleurotus ostreatus, Pleurotus cystidiosus, Mycobacterium tuberculosis.
References
- Anonymous.Global Tuberculosis Report. World health organization. 2020.
- Tuyiringire et al. Three promising antimycobacterial medicinal plants reviewed as potential sources of drug hit candidates against multidrug-resistant tuberculosis. Tuberculosis. 2020;124: 101987.
- Katalinic-Jankovic V, Furci L, Cirillo DM. Microbiology of mycobacterium tuberculosis and a new diagnostic test for TB. In European Respiratory Monograph, Edited by Hurst JR. European Respiratory Society, 2012; 8–13
- Yew WW, Lange C, Leung CC. Treatment of tuberculosis: Update 2010. European Respiratory Journal. 2010; 37:441–62.
- Chhipa et al. Nitroimidazoles: A newer class of Heterocycles for treatment of Tuberculosis. Research Journal of Pharmacy and Technology. 2020;13(7): 3425-32.
- Hosseinzadeh et al. The application of medicinal plants in traditional and modern medicine: A Review of Thymus vulgaris. International Journal of Clinical Medicine. 2015; 6: 635-42.
- Sanusi et al. Southeast Asian medicinal plants as a potential source of antituberculosis agent. Evidence-Based Complementary and Alternative Medicine. 2017; 39.
- Ngoci et al. Bioactivity of Cissampelos pareira medicinal plant against Mycobacterium tuberculosis. Journal of Pharmacognosy and Phytochemistry. 2015; 3(6): 167-173.
- Calixto et al. The Genus Psychotria: Phytochemistry, Chemotaxonomy, Ethnopharmacology and Biological Properties. Journal of the Brazilian Chemical Society. 2016; 27: 1355–78.
- Chowdhury et al. Pharmacological values and phytochemical analysis of aquatic plant Genus Aponogeton: A review. International Journal of Recent Innovations in Academic Research. 2019; 3(3): 125-41.
- Chowdhury NS, Farjana F, Sohrab H. Isolation, identification and pharmacological activities of endophytic fungi from Aponogeton undulatus Roxb. Pharmacology and Pharmacy, 2020; 11: 350-61.
- Jayasuriya et al. Hypoglycaemic activity of culinary Pleurotus ostreatus and P. cystidiosus mushrooms in healthy volunteers and Type 2 diabetic patients on diet control and the possible mechanisms of action. Phytotherapy Research. 2015; 29: 303-09.
- Jayasuriya et al. Anti-inflammatory activity of Pleurotus ostreatus, a culinary medicinal mushroom, in Wistar rats. Evidence Based Complementary and Alternative Medicine. 2020.
- Chatterjee et al. Antimycobacterial activities of an edible mushroom extract and its synergy with the standard antituberculous drugs. Journal of Clinical Diagnostic Research. 2018; 12: 37-45
- Afieroho et al. Antituberculosis and phytochemical investigation of the dichloromethane extract Pleurotus Tuber-Regium (Fries) Singer Sclerotium. International Research Journal of Pharmacy. 2013; 4(1): 255–57.
- Migliori et al. Drug resistance beyond extensively drug-resistant tuberculosis: individual patient data meta-analysis. European Respiratory Journal. 2013; 42: 169-179.
- Aro et al. In vitro antimycobacterial, apoptosis-inducing potential, and immunomodulatory activity of some Rubiaceae Species. Frontiers in Pharmacology. 2019; 10: 1–13.
- Ramos et al. Investigation of the antimycobacterial activity of 36 plant extracts from the Brazilian Atlantic forest. Brazilian Journal of Pharmaceutical Sciences. 2008; 44(4): 669–74.
- Moraes et al. Antimycobacterial activity and alkaloid prospection of Psychotria species (Rubiaceae) from the Brazilian Atlantic Rainforest. Planta Medica. 2011; 77(9): 964–70.
- Junior et al. Antimycobacterial and nitric oxide production inhibitory activities of triterpenes and alkaloids from Psychotria Nuda (Cham. & Schltdl.) Wawra. Molecules 2019; 24(6): 1–11.
- Ratnayake, WMKM. Anti-inflammatory activities of Psychotria sarmentosa and Acroynchia pedunculata leaves. Ph.D. Thesis, University of Sri Jayewardenepura, Sri Lanka. 2018.
- Teles et al. Effect of temperature on the degradation of bioactive compounds of Pinot Noir grape pomace during drying. Brazilian Journal of Food Technology. 2018; 21: e2017059.
- Sandhya M and Disha K. Expension in the field of Freeze-drying: An Advanced Review. Research Journal of Pharmacy and Technology. 2020; 13(5): 2468-74.
- Menikpurage IP, Soysa SSSBDP, Abeytunga DTU. Antioxidant activity and cytotoxicity of the edible mushroom, Pleurotus cystidiosus against hep-2 carcinoma cells. Journal of the National Foundation of Sri Lanka. 2012; 40(2): 106–13.
- Sanchez JGB and Kouznetsov VV. Antimycobacterial susceptibility testing methods for natural products research. Brazilian Journal of Microbiology. 2010; 41: 270-77.
- Famewo et al. Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa. African Health Sciences. 2017; 17(3): 780–89.
- Betoni et al. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Memórias do Instituto Oswaldo Cruz. 2006; 101(4): 387-90.