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Kowsalya, E.
- Evaluation of Genetic Diversity among Ocimum sanctum
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1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
1 Department of Industrial Biotechnology, BIHER, Selaiyur, Chennai-600073, IN
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Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2889-2894Abstract
In this study the evaluation of genetic diversity among Ocimum sanctum was undertaken. The method used to find variation among Ocimum sanctum was restriction fragment length polymorphism excluding blot hybridization. The Ocimum sanctum plants were taken from five different regions of Tamil Nadu - Chennai, Karaikudi, Kancheepuram, Vellore, Tanjavur and Puducherry. Along with whole plants the soil and water samples in the regions of the plant was also collected. DNA was isolated using CTAB protocol with ammonium acetate and ethanol precipitation. Then the DNA was quantified and restriction digested with EcoR1 and HindIII (double digested). Double digested Lambda DNA (EcoRI and Hind III) was used as marker and the samples along with marker were electrophoresed in 0.8% agarose gel at 80V and the bands were observed in UV trans illuminator. Based on observation of bands the plants from Karaikudi, Tanjavur, Kancheepuram and Puducherry may have been descended from Chennai plants and Vellore plant may have been descended from Karaikudi plant. There was sufficient variation to predict the migration pattern of the plant. And variation among water-hardness and pH and soil-N, P, K content and pH was also diverse. The measured soil and water characteristics may be few of the reasons for the variation among Ocimum sanctum around Tamil Nadu and Puducherry.Keywords
Ocimum sanctum, CTAB, DNA Extraction, Restriction Digestion, Nutrient of Soil And Hardness.
References
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- Bioremediation of Nitrate Reduction Present in Leather Industries Effluent by using Marine Algae
Abstract Views :289 |
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Authors
Affiliations
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, 173, Agaram Main Road, Selaiyur, Chennai-600 0073, Tamil Nadu, IN
1 Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, 173, Agaram Main Road, Selaiyur, Chennai-600 0073, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3522-3526Abstract
Nitrogen occurs naturally under oxidation of nitric anhydride, N2O5 and nitrates, NO3- and also from ammonia, and ammonium ions, NH4+. Because of increase in human activities environmental nitrate concentrations also increased which leads to introduction of large quantities of nitrates into ground and surface waters. Agriculture is the major source of nitrate pollution because of using of nitrogen-containing fertilizers, concentrated livestock and poultry farming. Nitrates are also released from the industrial production of glass, explosives, as well as from various chemical production and separation processes. Highest reduction in nitrate content was found in benzene extract of U.lactuca 91% and very minimal reduction of 12 % was found in aqueous extract of E.intestinalis. Among all, the extracts of Ulva lactuca, Enteromorpha flexuosa fell under Category A (Good reduction), and benzene was found to be the best solvent system. Maximum reduction by powdered algae was found in C.antenina collected from Covelong and Puduchery (375 mg/l).Keywords
Nitrate, Reduction, Bioremediation, Leather Effluent, Seaweed.References
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