Research Journal of Pharmacy and Technology

Doungue Takwete Herve ¹, Gaurav Kumar ¹

Affiliations
1 Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, IN

Abstract

Staphylococcus aureus is a very common food born pathogenic bacteria found in raw and under cooked chicken meat samples and its consumption may lead to the infection and/or toxicity in consumers. Therefore, this study was designed to study the prevalence and antibiotic sensitivity pattern of S. aureus in chicken meat samples collected from, retail slaughter houses at Jalandhar, Punjab, India. During this study, 84 fresh raw chicken meat samples were inoculated in mannitol salt agar for the selective isolation of S. aureus, later were characterized by a combination of microscopic and biochemical tests. Results of the study revealed that the out of 84 samples, 40 samples were containing S. aureus, which is 46.61% of total samples. Further, S. aureus isolates showed resistant towards more than two antibiotics, while few isolates were found to be multiple drug resistance (MDR). These results suggest that S. aureus is highly prevalent in retail meat in Jalandhar and many isolates are resistant to commonly used drugs.

Keywords

Chicken Meat, Staphylococcus aureus, Antibiotic Sensitivity.

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Ashish Vyas ¹, Karamjeet Singh ¹, Gaurav Kumar ¹

Affiliations
1 Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144402, Punjab, IN

Abstract

Urinary tract infection (UTI) is a very common and prevalent type of bacterial infection in females, therefore current study was designed to investigate the prevalence of microorganisms in female UTIs cases and their resistance patterns. During the study, a total of 281 urine samples were collected from females clinically suspected for urinary tract Infections (UTIs) and processed for the isolation and characterization of bacteria by classical cultural methods. Following this, these isolates were subjected to antimicrobial susceptibility testing by agar disk diffusion method to study their drug resistance patterns. Among 58 microbes isolated from the patients, Escherichia coli (58.62%) was the most prevalent etiological agent followed by Klebsiella sp. (8.62%), Staphylococcus aureus (5.17%), Enterococcus sp. (3.44%) and Enterobacter sp. (3.44%), while Candida albicans was also highly prevalent with 20.68% of UTI cases. Antimicrobial susceptibility analysis of uropathogens exhibited maximum resistant towards nalidixic acid while minimum against netillin (8.69%). Based on the results it can be concluded that E. coli is one of the most prevalent etiological agent of UTIs and a large number of uropathogens exhibits resistant towards multiple antibiotics, therefore its extremely important to know the sensitivity data for UTIs to initiate an effective empirical therapy.

Keywords

Antibiotic Resistance, Urinary Tract Infection, Escherichia coli, Uropathogens, Empirical Therapy.

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Wazbir Hussain Barlaskar ¹, Neeta Raj Sharma ¹, Gaurav Kumar ¹, Sanjeev Kataria ²

Affiliations
1 Lovely Professional University, Phagwara, Punjab, IN
2 Herbal Health Research Consortium, Amritsar, Punjab, IN

Abstract

Present study was aimed to analyze berberine concentration in Tinospora cordifolia stems collected from Ficus, Azadirachta, Syzygium, Populus and Eucalyptus. High Performance Liquid Chromatography equipped with UV detector was used for analytical study of berberine from the stem of Tinospora cordifolia. Methanol (100%) as a diluent was used for sample extraction. Using potassium dihydrogen phosphate and acetonitrile as a mobile phase in a ratio of 65 to 35 respectively and Zorbax XDB C-18, 250x 4.6mm 5-micron column, berberine was separated at 254nm wavelength. Maximum concentration of berberine was detected in the stem of Tinospora cordifolia climbed on Ficus (0.087%) followed by Azadirachta (0.084%). The least concentration of berberine was quantified in Eucalyptus (0.011%) on dried wt. basis.

Keywords

Berberine, Tinospora cordifolia, HPLC Chromatograms.

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