Research Journal of Pharmacy and Technology

Sanaa K. Gomaa ¹, Rania A. Zaki ¹, Heba A. El-Refai ¹, Reham R. El-Behery ²

Affiliations
1 Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Cairo, 12622,, EG
2 National Center for Radiation Research and Technology., EG

Abstract

Green synthesis of MgO nanoparticles (MgO NP) is a facile method that can be easily used for various biomedical, agricultural, catalysis, physics, chemistry, and electronic fields. The construction of nanoparticles by this method makes it compatible with antibacterial studies. Statistical analysis was employed to derive a statistical model for the individual effect of the physicochemical conditions affecting the production conditions of MgO NP to upturn the dry weight to 3 mg /50 ml, which is 6.66 times larger than a basal condition (0.45 mg /50ml). The biosynthesized MgO NP was characterized using UV-VIS, FT-IR, XRD, and TEM. The green synthesized MgO NP killed both gram positive and gram-negative bacteria and disrupted nascent biofilms thus could be used as a potential nanomaterial for in vivo applications such as coating for a medical implant, suggesting new antimicrobial mechanisms. Apart from this, the work reported here stands out from others in various aspects. First, this is the first report (to the best of our knowledge), which calculates the yield of synthesized MgO NP by Bacillus paramycoides strain MCCC 1A04098. Secondly, conditions for optimal yield, different particle sizes of MgO NP were screened using Plackett Burman design and confirmed by UV-VIS spectroscopy, FT-IR, and XRD.

Keywords

MgO NP, antibacterial, UV-VIS spectroscopy, FT-IR technique, and X-ray Diffraction (XRD).

Full Text