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Sivakumar, J.
- Comparative study of Probiotic Bacteria on ornamental fish giant gourami, Osphronemus goramy for its survival and growth
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1 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
2 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
3 Universiti Sultan ZainalAbidin, Terengganu, MY
1 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
2 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
3 Universiti Sultan ZainalAbidin, Terengganu, MY
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 262-268Abstract
Production of high quality giant gourami is considered essential to prevent the disease outbreak, survival and growth at aquarium. This paper deals with the production of high quality giant gourami, Osphronemus goramy using probiotics. Materials and Methods: This experiment was designed in such way that stocking the Giant gourami in fourgroups, the first one as control without probiotics and the second as experimental with Bacillus sp, third with Lactobacillus spand the fourth as consortium; a mixture of Bacillus sp and Lactobacillus sp. Results and Discussion: The results were compared in terms of quality of Giant gourami. The fish treated with consortium exhibited highest growth rate (Body Weight-4.13+8.24 gm) and survival of 95% at Total Length– 4.23+8.47 cm stage. The upper most growth rate was noted in group IV treated daily with consortium. This treatment group improved in mean weight from 4.13 to 8.27 with an average growth rate of 0.069% per day. This was significantly better than the control group I which grew from 4.15 to 5.83 g with an average of 0.028 % per day. In the experimental tanks the water treated with probiotics played a key role in maintaining the water quality parameters and keeping the ammonia level less than 1.0 ppm. Conclusion: It was established that the use of probiotics plays a very important role in maintaining the water quality, survival and growth rate in ornamental fish forming systems.Keywords
Bacillus sp, consortium, Giant gourami, Lactobacillus sp, Osphronemus goramy, Probiotics.References
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- Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala
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Authors
Affiliations
1 Universiti Sultan Zainal Abidin, 20400 Terengganu, MY
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, IN
3 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, IN
1 Universiti Sultan Zainal Abidin, 20400 Terengganu, MY
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, IN
3 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 297-302Abstract
Objectives: Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala. Materials and Methods: The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds. Results: Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids.The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis. Conclusion: Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.Keywords
Brassica oleracea var acephala, Phytochemical Screening, Antibacterialactivities, GC-MS Analysis, FTIR Analysis.References
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- Relative Bioremediation of Used engine oil Contaminated soil from an Industrialised area by Various microbes
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Authors
Affiliations
1 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603 103, IN
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603 103, IN
3 Universiti Sultan Zainal Abidin, 20400 Terengganu, MY
1 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603 103, IN
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603 103, IN
3 Universiti Sultan Zainal Abidin, 20400 Terengganu, MY
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 331-338Abstract
Aim:The present study is to degrade the engine oil through microbes from the industrialized areas in and around Chrompet, Chennai. Engine oil contaminated soil samples were collected from different locations of Chrompet which is located 35 KM away from Chennai. Methods:As a result of spread plate technique the microbial colonies were enumerated and three different organisms such as bacteria namely Pseudomonas sp and Bacillus sp.Actinomycetesnamely Streptomyces spand funginamely Aspergillus flavus and Aspergillus nigerwereidentified. The bacterial colonies were identified by morphological and biochemical techniques using the taxonomic scheme. pH and optical density (growth rate) was determined for Pseudomonas sp, Bacillus sp, consortium (Pseudomonas sp and Bacillus sp), Streptomyces sp,Aspergillusflavus and Aspergillus nigerto the concentration from 1ml to 5ml for 16 days. Results:All the isolated microorganisms had the potential to degrade the engine oil. Comparatively the bacterial consortium had the highest potential to degrade the engine oil.Summary:This formulation may be the best solution to solve the land pollution by engine oil and also to conserve the natural environment.Keywords
Aspergillus flavus, Aspergillus niger, Bacillus sp, Consortium, Engine oil, Pseudomonas sp, Streptomyces sp.References
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- In Silico Analysis of the Betuline from the Fiddler Crab, Uca annulipes and its Antimicrobial as Well as Anti Lung Cancer Activities.
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Authors
Affiliations
1 Universiti Sultan ZainalAbidin, Terengganu, MY
2 Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
3 Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
1 Universiti Sultan ZainalAbidin, Terengganu, MY
2 Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
3 Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai – 603 103, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 4 (2019), Pagination: 1849-1856Abstract
Introduction: <i>In silico</i> analysis of the bioactive compound betuline from the fiddler crab, Uca annulipes and its antimicrobial activities were studied. The fiddler crabs, Uca annulipes were collected from Muttukadu Estuary located on the Kanchipuram District, Tamil Nadu, India, 35 K.M away from Chennai, on the East Coast Road route to Kovalam. Materials and Methods: Fiddler crabs were collected by hand picking method. From the muscle mass of the Crab the bioactive compound was isolated by GC-MS analysis. Molecular docking was performed to identify the protein ligand responsible for the affinity with lung cancer causing tumour cells. Results and Discussion: Hence betuline is the potential lead molecule for the inhibition of lung cancer protein and the most important residues for potential drug target as carbon hydrogen bond, conventional hydrogen bond and Vander Waals interaction. In vitro studies of antimicrobial activities clearly indicated that the different concentrations of betuline bioactive compound have the potential to control the bacteria such as Escherichia coli and Pseudomonas aeruginosa. In vitro studies of anticancer activities also evidently showed the different concentrations of bioactive compound betuline have the potential to control the proliferation of lung cancer cells. Conclusion: Reports are very scanty on this species studied and the reports are very old and hence this present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs against the lung cancer. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the lung cancer.Keywords
Betuline, Escherichia coli, Lung Cancer, Pseudomonas aeruginosa and Uca annulipes.References
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- In Silico Analysis of the Phenylephrine from the Sand Crab (Emerita asiatica) for its Antimicrobial Activities
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Authors
Affiliations
1 Universiti Sultan Zainal Abidin, Terengganu, MY
2 Research Department of Biotechnology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN
3 Research Department of Microbiology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN
1 Universiti Sultan Zainal Abidin, Terengganu, MY
2 Research Department of Biotechnology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN
3 Research Department of Microbiology, Hindustan College of Arts and Science, Padur, Chennai – 603103, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2145-2152Abstract
Aim: The present investigation gives a detailed report on the analysis of the bioactive compound isolated from the sand crab Emerita asiatica is having better relation with vasoconstriction. Methods: The sand crab Emerita asiatica were collected from Thiruvidandhai beach located on the Kanchipuram District, Tamil Nadu, India, 41 K.M away from Chennai, on the East Coast Road route to Mahabalipuram. Sand crabs were collected by hand picking method. From the muscle mass of the Crab the organic compound was isolated by GC-MS analysis. Molecular docking was also performed to identify the protein ligand responsible for the constriction of blood vessels. Results: The isolated bioactive compound was identified as phenylephrine, an α1 adrenergic receptor which has the potential to lead the molecule for the vasoconstriction. In vitro studies of antibacterial activities clearly indicated that the different concentrations of phenylephrine have the antibacterial activity against the bacteria such as Klebsiella pneumoniae and Staphylococcus aureus. Summary: The present investigation would give latest information on the isolation of bioactive compound for the production of pharmaceutical drugs from marine organisms. These results will be decisive factor for determining a lead bioactive compound for further drug discovery process for the vasoconstriction.Keywords
Emerita asiatica, Klebsiella pneumoniae, Phenylephrine, Staphylococcus aureus, Vasoconstriction.References
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