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Vijayalakshmi, K.
- Evaluation of Anticancer activity of Ethanol extract of Bauhinia tomentosa Linn. on A549, Human Lung Carcinoma Cell lines
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Authors
Affiliations
1 Department of Biochemistry, Bharathiar University. Coimbatore–641046, IN
2 Department of Biochemistry, Bharathi Women’s College, Chennai–600108, IN
1 Department of Biochemistry, Bharathiar University. Coimbatore–641046, IN
2 Department of Biochemistry, Bharathi Women’s College, Chennai–600108, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2748-2752Abstract
Objective: The aim of the present study is to evaluate the anticancer activity of ethanol extract of B.tomentosa (EBT). Methods: The anticancer activity of ethanol extract of B.tomentosa was evaluated by MTT Assay, AO/EtBr Fluorescence assay and DNA Fragmentation assay against A549 (human lung cancer cell line). Results: Treatment of A549 cells with EBT (at IC50 concentration) for 48 hrs significantly inhibited the proliferation in a concentration dependent manner. AO/EtBr staining confirms the occurrence of apoptosis in A549 cells after the treatment of EBT. From DNA fragmentation assay, it is evident that EBT has also significantly induced DNA damage in treated A549 cells. Conclusion: Thus the present study demonstrates the anticancer activity of EBT. The ethanol leaf extract of B.tomentosa has shown potent anticancer activity on A549 cancerous cell line. Further studies are needed to identify the bioactive molecules and to explore its mechanism of action which might throw light in the development of new alternative drug for lung cancer.Keywords
Anticancer Activity, B.tomentosa, MTT Assay, AO/Et Br, DNA Fragmentation, A549.
References
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- Effect of Punica granatum peel and Vitis vinifera seeds on Biochemical Indices in DEN induced Hepatocellular Carcinoma in Rats
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Authors
Affiliations
1 Department of Biotechnology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamilnadu, IN
2 Department of Biochemistry, Bharathi Women’s College, Chennai, Tamilnadu, IN
1 Department of Biotechnology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamilnadu, IN
2 Department of Biochemistry, Bharathi Women’s College, Chennai, Tamilnadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2758-2764Abstract
Plants are the richest source of traditional medicines in treatment against many diseases. Many of the plant materials wasted are the reservoir of phytochemicals possessing important biological activities. In this present study, the extracts of Punica granatum peel and Vitis vinifera a seeds were evaluated in rats against (diethylnitrosamine) DEN induced hepatocellular carcinoma for assessing the hepatoprotective potential. Rats were divided into four groups. The first group served as normal control group, the second group received DEN at a dose of 200 mg/kg body weight by Single intraperitoneal administration. The third one received DEN as in DEN-treated group and co-treated with 400 mg/kg Punica granatum peel extract (EPGP). The final group also received DEN and co-treated with 400 mg/kg Vitis vinifera seeds extract (EVVS). DEN administration to rats resulted in significantly elevated levels of serum AFP, bilirubin, triglycerides, cholesterol and urea. The levels of plasma total protein, albumin and blood glucose were significantly decreased in DEN treated rats. No significant changes were noted in creatinine levels of DEN induced rats. Co-treatment with the Punica granatum peel and Vitis vinifera seed extracts orally for 12 weeks significantly reversed the DEN induced alterations of above mentioned parameters in the serum. The present study indicates that both the selected extracts exhibits good hepato protectivity against DEN induced hepatocellular damage in rats.Keywords
Vitis vinifera, Punica granatum, Diethylnitrosamine, Alpha Feto Protein, Hepatocellular Carcinoma.References
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