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Das, Nilanjana
- Application of Gum Based and Clay Based CuO/Chitosan Nanobiocomposite Beads for the Removal of Nickel(II) from Aqueous Environments:Equilibrium, Kinetic, Thermodynamic and ex-Situ Studies
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Authors
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, TamilNadu, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, TamilNadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 5 (2017), Pagination: 1347-1359Abstract
The present investigation is focused on the removal of Ni(II) ions from aqueous environment using gum (GA) based and clay (MMT) based nanobiocomposite beads composed of CuO NPs and chitosan (Ch). The potential of beads was exploited in terms of pH (2.0-10.0), contact time (2-36 h), temperature (10-50 °C), initial metal concentration (100-1200 mg L-1) and adsorbent dosage (1.0-6.0 g L-1) during the adsorption process. Under optimized condition, maximum Ni(II) removal (95.05%) was exhibited by clay based nanobiocomposite (CuO-Ch-MMT) beads followed by gum based nanobiocomposite (CuO-Ch-GA) beads (90.12%). Equilibrium data showed the best fit to Langmuir model with the highest adsorption capacity suggesting a homogeneous mode of Ni(II) adsorption onto the nanobiocomposite beads. Kinetic studies showed better applicability of pseudo-first order model suggesting physisorption as the underlying phenomena. Thermodynamic studies showed that the process was endothermic and spontaneous. The adsorption mechanism of CuO-Ch-GA and CuO-Ch-MMT beads was further elucidated using SEM, EDX and FT-IR analyses. Ex-situ studies showed a maximum Ni(II) removal of 88.26% from electroplating wastewater using CuO-Ch-MMT beads in column mode. Regeneration studies suggested that CuO-Ch-MMT beads could be consistently reused up to 4 cycles.Keywords
Adsorption, Chitosan, Gum Arabic, Montmorillonite (MMT), Nickel(II).- Enhanced Removal of Dichlorvos from Aqueous Solution using zinc-silver Bimetallic Nanoparticles Embedded in Montmorillonite-Biopolymer Nanobiocomposites:Equilibrium, Kinetics and Thermodynamic Studies
Abstract Views :139 |
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Authors
K. Sahithya
1,
Nilanjana Das
1
Affiliations
1 School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1105-1114Abstract
The present investigation is focused on the application of bimetallic zinc-silver (Zn-Ag) nanoparticles embedded in montmorillonite (MMT)-polysaccharide (chitosan-Ch and gum ghatti-Gg) nanobiocomposites for the removal of dichlorvos (DCV) from aqueous solution. Operating parameters viz., pH (4.0-11.0), contact time (30-360 min), temperature (20-50°C), initial DCV concentration (20-140 mg/L), composite dosage (0.2-1.2 g/L) were optimized. Zn-Ag/MMT/Ch nanobiocomposite showed the maximum removal of DCV (96.6 %) followed by Zn-Ag/MMT/Gg (85.3 %) nanobiocomposite and Zn-Ag/MMT (64.3 %) nanocomposite. The process followed a heterogenous mode of DCV adsorption by all the composites. The kinetic studies indicated that the DCV adsorption followed pseudo-first order model. Intraparticle diffusion and Boyd plot suggested that the film diffusion was not the sole rate limiting step. The thermodynamic parameters indicated the feasibility and exothermic nature of DCV adsorption. The mechanism of adsorption process was further elucidated by FT-IR, AFM and EDX analysis. Packed bed column studies showed the removal of DCV up to 79.3 % using Zn- Ag/MMT/Ch under 12 cm bed height, 1 ml/min of flow rate and at 0 % dilution. Regeneration studies suggested that the Zn-Ag/MMT/Ch could be reused up to three cycles.Keywords
Adsorption, Biopolymer, Dichlorvos (DCV), Montmorillonite (MMT), Zinc-Silver Nanoparticles (Zn-Ag).- Isolation, Identification and In vitro Characterisation of Probiotic Yeast Strains
Abstract Views :156 |
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Authors
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 726-732Abstract
In the present study, ten yeasts were isolated from fruit juices and subjected to in vitro tests for screening probiotic potentiality. Three isolates exhibited higher tolerance for pH and heat stress. Based on preliminary screening, isolates were subjected to molecular identification and identified as Wickerhamomyces anomalusVITASN01, Saccharomyces cerevisiae VIT- ASN03 and Yarrowia lipolytica VIT- ASN04. The screened yeast isolates survived and grew well at human gastrointestinal conditions in pH 2.5 and 0.3% (w/v) oxgall at 37°C. The antagonistic activity of yeast isolates were tested against clinical pathogens, viz.S. typhii, B. subtilis and E. coli. W. anomalusVIT-ASN01 showed maximum tolerance to high bile salt concentration and acidity, could remove cholesterol 51.28% during 48 h and produced 586.55 mg/L of exopolysaccharide. It can be concluded that the screened novel yeast isolates can serve as promising probiotics in various fields of food industry.Keywords
Bile Tolerance, Cholesterol Assimilation, Probiotics, Yeast.- Optimization and Characterization of Polyhydroxyalkanoates and its Copolymers Synthesized by Isolated Yeasts
Abstract Views :146 |
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Authors
Nupur Ojha
1,
Nilanjana Das
1
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 861-868Abstract
The present study investigates the role of yeasts, isolated from different environments for its abilities to synthesize biodegradable polymer, Polyhydroxyalkanoates (PHA) and its copolymers. Serial dilution method and spread plate technique had been used for the isolation of yeasts from different samples. Screening had been done by crotonic assay followed by UV Spectrophotometric analysis. Growth parameters such as effect of carbon sources, incubation period, inoculum percentage, incubation temperature and pH of the production medium were optimized for each of the screened isolates. Characterization of PHA was done by FTIR and GCMS analysis. Maximum PHA produced was observed after 96 hours of incubation period inoculated with 2% (v/v) of inoculum in minimal media of pH 8 containing various carbon sources viz. 5% sucrose for Isolate1; 5% glucose for Isolate 2; 3% starch for Isolate 3 and 5% Lactose for Isolate 4. Incubation temperature was maintained at 37°C. Maximum PHA production was noted as 40 % in Isolate 1, 33% in Isolate 2, 29% in Isolate 3and 25% in Isolate 4 respectively on the basis of dry cell weight under optimized condition. Prominent peaks obtained through FTIR analysis at wavelength 3273, 2922, 1739 and 1462 cm -1 showed the presence of stretching bands of -OH-,-CH-, -C=O- and -CH3- group which are the characteristic peaks of PHA polymer. GC-MS chromatogram detected the peaks at retention time 3.063 and 19.25 min were corresponding to the derivatized products of butanoic acid (polyhydroxybutyrate) and octadecanoic acid (polyhydroxyoctadecanoate).The present study demonstrated that yeast Isolate 1 could produce high amount of PHA (40% on dry cell weight basis ) with hydroxybutyrate (HB) and hydroxyoctadecanoate (HOD) as its main constituents.Keywords
Yeast Isolates, Optimization, FTIR, GC-MS, Polyhydroxybutyrate, Polyhydroxyoctadecanoate.- Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 83-92Abstract
The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO3, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO3 at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).Keywords
Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.References
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- A Green Approach Towards Utilization of Floral Wastes for the Extraction of Natural Colorants
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 269-279Abstract
The present study is focused on the utilization of floral wastes for the extraction of natural colorants. Waste flowers were collected from various sites of temples, garden, domestic and decorative areas and identified as Rosahybrid, Tageteserecta, Spathodeacampanulata, Bougainvilleaglabra, and Euphorbiamili. Identification and characterization of extracted colorants was done using thin layer chromatography (TLC), UV Spectroscopy andFourier Transform Infra-red spectroscopy (FT-IR) analysis. Antimicrobial and antioxidant properties of extracted natural colorants were evaluated. Applicability of extracted colorants as natural dye and biofertilizer was also evaluated. It can be concluded that the natural colorants extracted from the floral wastes can be used in textile and pharmaceutical industries.In addition, contamination free natural colorants can also serve as safe biofertilizer.Keywords
Floral Wastes, Natural Colorants, Antimicrobial, Antioxidant, Natural Dye, Biofertilizer.References
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- Applications of Microbial Bioflocculants for Environmental Remediation: An Overview
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Authors
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT, Vellore 632014, Tamil Nadu,, IN
2 Department of Biotechnology, Chaitanya Bharathi Institute of Technology, Hyderabad - 500075, Telangana,, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT, Vellore 632014, Tamil Nadu,, IN
2 Department of Biotechnology, Chaitanya Bharathi Institute of Technology, Hyderabad - 500075, Telangana,, IN
Source
Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1883-1890Abstract
The application of bioflocculants has recently become a promising solution for the treatment of water and wastewater as well as removal of pollutants from environment. Water pollution is the most challenging environmental issue in the developing countries to determine the quality of life. The wastewater from different sources contains suspended solids, organic and inorganic particles, dissolved solids, heavy metals, dyes and other impurities which are harmful to the environment causing major health hazards in human and animals. The use of bioflocculants is advantageous for the control of environmental pollution as they are non- toxic and biodegradable in nature. Moreover, they do not create any secondary pollution. Chemical flocculants being a source of carcinogens can be replaced by bioflocculants which needs to be produced on a large scale. However, commercially viable bioflocculants are yet to be produced and marketed widely. This review intends to present the updated information on microbial bioflocculants and their applications for remediation of pollutants from wastewater. It may bring up the significant issues which can be attempted by future researchers for a better understanding to develop commercially viable, safe, eco-friendly and cost effective bioflocculants using new biotechnological techniques.Keywords
Bioflocculant, Biodegradable, Eco-Friendly, Flocculation Activity, Wastewater Treatment.References
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