Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Azizah, Norma Nur
- The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line
Abstract Views :150 |
PDF Views:0
Authors
Ratu Shafira Hanifah
1,
Nadia Aida Novitarani
1,
Fadhilah Harmen
1,
Aryo Tedjo
2,
Norma Nur Azizah
2,
Rista Putrianingsih
2,
Wilzar Fachri
3,
Kusmardi Kusmardi
4
Affiliations
1 Universitas Indonesia, Salemba, Jakarta, ID
2 Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
4 Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, ID
1 Universitas Indonesia, Salemba, Jakarta, ID
2 Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
4 Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2902-2906Abstract
Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1,327 μg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173,33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.Keywords
COX-2, Colorectal Cancer, IC50, Immunocytochemistry, MTT Assay, Phaleria macrocarpa.
References
- PubMed Health [Internet]. Cancer (Malignant neoplasm). Bethesda: U.S National Library of Medicine; 2016 [cited 2018 Jul 17]. Available from: https://www.ncbi.nlm.nih.gov/pubmedhealth/PMHT0015630/
- Depkes.go.id [Internet]. Situasi Penyakit Kanker. Jakarta: Indonesian Ministry of Health; 2015 [cited 2018 Jul 17] Available from: http://www.depkes.go.id/resources/download/pusdatin/infodatin/infodatin-kanker.pdf
- Cancer [Internet]. Geneva: World Health Organization; 2018 [cited 2018 Jul 17]. Available from: http://www.who.int/news-room/fact-sheets/detail/cancer
- Kemkes.go.id [Internet]. Kanker kolorektal. Jakarta: Indonesian Ministry of Health; 2017. Available from: http://kanker.kemkes.go.id/guidelines/PNPKkolorektal.pdf
- Abdullah M, Sudoyo AW, Utomo AR, Fauzi A, Rani AA. Molecular profile of colorectal cancer in Indonesia: is there another pathway ?. Gastroenterol Hepatol Bed Bench. 2012; 5(2): 71–8.
- Roelofs HMJ, Morsche RHM, Heumen BWH, Nagengast FM, Peters WHM. Over-expression of COX-2 mRNA in colorectal cancer. BMC Gastroenterology. 2014; 14:1.
- Cuzick J, Otto F, Baron JA, Brown PH, Burn J, Greenwald P, et al. Aspirin and non-steroidal anti-inflammatory drugs for cancer prevention: An international consensus statement. Lancet Oncol. 2009; 10: 501-7.
- Shwter AN, Abdullah NA, Alshawsh MA, El-Seedi HR, Al-Henhena NA, Khalifa SA, et al. Chemopreventive effect of Phaleria macrocarpa on colorectal cancer aberrant crypt foci in vivo. J Ethnopharmacol. 2016 Dec 4; 193:195-206.
- Yeung TM, Gandhi SC, Wilding JL, Muschel R, Bodmer WF. Cancer stem bark cells from colorectal cancer-derived cell lines. Proc Natl Acad Sci U S A. 2010 Feb 23; 107(8): 3722–3727.
- Sriwirajayan N, Ninpesh T, Sukpondma Y, Nasomyon T, Graidist p. Cytotoxicity screening of plants of genus Piper in breast cancer cell lines. Trop J Pharm Res. 2014;13(6):921-8.
- Hinkle DE, Wiersma W, Jurs SG. Applied statistics for the behavioral sciences. 5th Ed. Boston: Houghton Mifflin; 2003.
- Gurbuz V, Yilmaz A, Gokçe O, Konac E. The apoptotic effects of cisplatin on human colon cancer cell line (HT29). Drug Metab Rev. 2010 Aug;42(2):196-7.
- Son DJ, Hong JE, Ban JO, Park JH, Lee HL, Gu SM, et al. Synergistic inhibitory effects of cetuximab and cisplatin on human colon cancer cell growth via inhibition of the ERK-dependent EGF receptor signaling pathway. Biomed Res Int. 2015; 2015:397563.
- Rahmawati E, Dewoto HR, Wuyung PE. Anticancer activity study of ethanol extract of Mahkota dewa fruit pulp (Phaleria macrocarpa (Scheff.) Boerl.) in C3H mouse mammary tumor induced by transplantation. Med J Indones. 2006 Nov 1;15(4):217-2.
- Shwter AN, Abdullah NA, Alshawsh MA, El-Seedi HR, Al-Henhena NA, Khalifa SA, Abdulla MA. Chemopreventive effect of Phaleria macrocarpa on colorectal cancer aberrant crypt foci in vivo. J Ethnopharmacol. 2016 Dec; 193:195-206.
- Khalek FJ, Gallicano GI, Mishra L. Colon cancer stem bark cells. Gastrointest Cancer Res. 2010 Nov-Dec; (Suppl 1): S16–23.
- Mustafa M, Menon J, Muniandy RK, Illzam EL, Shah MJ, Sharifa AM. Colorectal cancer: pathogenesis, management and prevention. IOSR JDMS. 2016 May;15(5):94-8.
- Sostres C, Gargallo CJ, Lanas A. Aspirin, cyclooxygenase inhibition and colorectal cancer. Worl J Gastrointest Pharmacol Ther. 2014 Feb;5(1):40-9.
- Wang D, DuBois RN. The role of COX-2 in intestinal inflammation. Oncogene. 2010 Feb; 29(6):781-8.
- Kusmardi K, Estuningtyas A, Shavera D, Tedjo A, Priosoeryan to B, Habiburrahman M, et al. The effect of mahkota dewa (Phaleria macrocarp) (Scheff.) fruit pericarp extract on COX-2 and B-catenin expression in mice colon induced by dextran sodium sulfate. Pharmacology Online. 2018;2(2):199-205.
- Lay MM, Karsani SA, Banisalam B, Mohajer S, Malek A, Nurestri S. Antioxidants, phytochemicals, and cytotoxicity studies on Phaleria macrocarpa (Scheff.) Boerl seeds. BioMed Res Intl. 2014;2014.
- Wray V, Kahl R, Wa W, Michels G, Niering P, et al. J Pharm Pharmacol. Polyphenols from plants used in traditional Indonesian medicine (Jamu): uptake and antioxidative effects in rat H4IIE hepatoma cells. Journal of pharmacy and pharmacology. 2005 Feb;57(2):233-40.
- He Z, Chen AY, Rojanasakul Y, Rankin GO, Chen YC. Gallic acid, a phenolic compound, exerts anti-angiogenic effects via the PTEN/AKT/HIF-1α/VEGF signaling pathway in ovarian cancer cells. Oncol rep. 2016 Jan;35(1):291-7.
- Susilawati S, Matsjeh S, Pranowo HD, Anwar C. Antioxidant activity of 2, 6, 4’-trihydroxy-4-methoxy benzophenone from ethyl acetate extract of leaves of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.). Indonesian Journal of Chemistry. 2011 Nov 3;11(2):180-5.
- Winarno H. Benzophenone glucoside isolated from the ethyl acetate extract of the bark of mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] and its inhibitory activity on leukemia L1210 cell line. Indonesian Journal of Chemistry. 2010 Jun 20;9(1):142-5.
- Lee KM, Lee KW, Jung SK, Lee EJ, Heo YS, Bode AM, Lubet RA, Lee HJ, Dong Z. Kaempferol inhibits UVB-induced COX-2 expression by suppressing Src kinase activity. Biochem pharmacol. 2010 Dec 15;80(12):2042-9.
- García-Mediavilla V, Crespo I, Collado PS, Esteller A, Sánchez-Campos S, Tuñón MJ, González-Gallego J. The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang Liver cells. European journal of pharmacology. 2007 Feb 28;557(2-3):221-9.
- Amaravani M, Prasad NK, Ramakrishna V. COX-2 structural analysis and docking studies with gallic acid structural analogues. Springer Plus. 2012 Dec 1;1(1):58.
- Shen YC, Chen SL, Zhuang SR, Wang CK. Contribution of tomato phenolics to suppression of COX‐2 expression in KB cells. Journal of food science. 2008 Jan;73(1):C1-0.
- Synthesis Nanoparticle extract of Clove (Syzygium aromaticum L.) and Characterization by Differential Scanning Colorimetry Profiling and its Activities as Inhibitor of HeLa Cell lines
Abstract Views :335 |
PDF Views:0
Authors
Fadilah
1,
Vallas Aditiar Widodo
2,
Rahardi Prasetia Priawan
2,
Ericko Ongko Joyo
2,
Hadin Abdurrohman
2,
Fatmawaty
1,
Rafika Indah Paramita
1,
Norma Nur Azizah
3
Affiliations
1 Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
2 Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
1 Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
2 Medical Student, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3355-3358Abstract
Herbs and spices have been used for thousands of years as medicines. But their have hydrophobic properties, poor stabilities and low bio availabilities, nanoparticles are possible since the interaction of the particle surface with the solvent is strong enough to overcome density differences. DSC melting profiles are essential for identifying the state of the drug, which can significantly influence bioavailability, stability, and water content. The aim of research analysed of nanoparticle extract of Clove (Syzygium aromaticum L.) by Differential Scanning Colorimetry and their activity toward HeLa cell line. The result of synthesis showed that nanoparticle extract with yield 75% yield. In-vitro test results showed that the IC50 of nanoparticle extract and ethanol extract 6.2±2.1 μg/ml, 22.53±1.1μg/ml respectively. The results of this test indicate the synthesis of nanoparticle with traditional herbal medicine may provide a very useful tool in designing future herbal medicine with improved bioavailability profile and less toxicity. That the combination a high potential as a anti cervical cancer compound.Keywords
Nanoparticle, Extract Syzygium aromaticum L, DSC, HeLa Cell Line, MTT Assay.References
- Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C. Cancer Incidence and Mortality Worldwide: IARC Cancer Base No.11 [internet]. Lyon: International Agency for Research on Cancer. Globocan 2012 v1.0; Available from http://globocaniarc.fr.
- Ministry of Health Republic of Indonesia. Cancer Situation in Indonesia. Jakarta: Ministry of Health, 2015, 1-5
- Longo D, Harrison T. Harrison's hematology and oncology. New York: McGraw-Hill Education, 2013, 312-33.
- Campos NG, Sharma M, Clark A, Lee KE, Geng F, Kim FJ. Comprehensive Global Cervical Cancer Prevention: Costs and Benefits of Scaling Up within A Decade. Boston: Center for Health Decision Science Harvard School of Public Health, 2016; 8-12.
- Dimmer JLB, Hastert T, Silbiger D, Ruterbusch J, Cespedes E, Chlebowski RT, Manson JE. Features of The Metabolis Syndrome and Survival after A Diagnosis of Breasr Cancer in The Women’s Health Initiative (WHI). Journal of Clinical Oncology. 2016, 15: 1517.
- American Cancer Society. Treating Cervical Cancer. American Cancer Society, 2016:4-17.
- American Cancer Society. Treating Cervical Cancer. 2016: 5-10.
- Chaieb K, Hajlaoui H, Zmantar T, Kahla N, Rouabhia M, Mahdouani K, and Bakhrouf A. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzygium aromaticum L.). Phytother. Res. 2007, 21, 501-506.
- Liu S, Gong W, Dong Y, Hu Q, Chen X, Gao X. The effect of submicron bioactive glass particles on in vitro osteogenesis. RSC Adv. 2015, 5:38830–38836.
- Misra SK, Mohn D, Brunner TJ, Stark WJ, Philip SE, Roy I, Salih V, Knowles JC, Boccaccini AR. Comparison of nanoscale and microscale bioactive glass on the properties of P(3HB)/Bioglass® composites. Biomaterials. 2008, 29: 1750–1761.
- Michnik A, Drzazga Z, Kluczewska A, Michalik K. Differential scanning micrcalorimetry study of the thermal denaturation of haemoglobin. Biophysical Chemistry. 2005, 118(2-3): 93-101.
- Rajaonarivony M, Vauthier C, Couarraze G. Development of a new drug carrier made from alginate. J. Pharm. Sci. 1993, 82:912–917.
- Darling JL. Neuronal and glial tumours in vitro: An overview, in: Doyle, A., Griffiths, J.B. (Eds.), Cell and tissue culture for medical research. John Wiley and Sons Ltd, London, 2000, 306 – 320.