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Ojha, Nupur
- Optimization and Characterization of Polyhydroxyalkanoates and its Copolymers Synthesized by Isolated Yeasts
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Authors
Nupur Ojha
1,
Nilanjana Das
1
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 861-868Abstract
The present study investigates the role of yeasts, isolated from different environments for its abilities to synthesize biodegradable polymer, Polyhydroxyalkanoates (PHA) and its copolymers. Serial dilution method and spread plate technique had been used for the isolation of yeasts from different samples. Screening had been done by crotonic assay followed by UV Spectrophotometric analysis. Growth parameters such as effect of carbon sources, incubation period, inoculum percentage, incubation temperature and pH of the production medium were optimized for each of the screened isolates. Characterization of PHA was done by FTIR and GCMS analysis. Maximum PHA produced was observed after 96 hours of incubation period inoculated with 2% (v/v) of inoculum in minimal media of pH 8 containing various carbon sources viz. 5% sucrose for Isolate1; 5% glucose for Isolate 2; 3% starch for Isolate 3 and 5% Lactose for Isolate 4. Incubation temperature was maintained at 37°C. Maximum PHA production was noted as 40 % in Isolate 1, 33% in Isolate 2, 29% in Isolate 3and 25% in Isolate 4 respectively on the basis of dry cell weight under optimized condition. Prominent peaks obtained through FTIR analysis at wavelength 3273, 2922, 1739 and 1462 cm -1 showed the presence of stretching bands of -OH-,-CH-, -C=O- and -CH3- group which are the characteristic peaks of PHA polymer. GC-MS chromatogram detected the peaks at retention time 3.063 and 19.25 min were corresponding to the derivatized products of butanoic acid (polyhydroxybutyrate) and octadecanoic acid (polyhydroxyoctadecanoate).The present study demonstrated that yeast Isolate 1 could produce high amount of PHA (40% on dry cell weight basis ) with hydroxybutyrate (HB) and hydroxyoctadecanoate (HOD) as its main constituents.Keywords
Yeast Isolates, Optimization, FTIR, GC-MS, Polyhydroxybutyrate, Polyhydroxyoctadecanoate.- Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 83-92Abstract
The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO3, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO3 at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).Keywords
Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.References
- Eugenio, Mateus, et al. "Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria." RSC Advances 6.12 (2016): 9893-9904.
- BoroumandMoghaddam, Amin, et al. "Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medical applications." Molecules 20.9 (2015): 16540-16565.
- Gajbhiye, Monali, et al. "Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole." Nanomedicine: Nanotechnology, Biology and Medicine 5.4 (2009): 382-386.
- Nayak, RatiRanjan, et al. "Green synthesis of silver nanoparticle by Penicilliumpurpurogenum NPMF: the process and optimization." Journal of Nanoparticle Research 13.8 (2011): 3129-3137.
- Kowshik, Meenal, et al. "Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3." Nanotechnology 14.1 (2002): 95.
- Shahverdi, Ahmad R., et al. "Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli." Nanomedicine: Nanotechnology, Biology and Medicine 3.2 (2007): 168-171.
- Durán, Nelson, et al. "Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains." Journal of nanobiotechnology 3.1 (2005): 8.
- Ghodake, Gajanan, Yeong Deuk Seo, and Dae Sung Lee. "Hazardous phytotoxic nature of cobalt and zinc oxide nanoparticles assessed using Allium cepa." Journal of hazardous materials 186.1 (2011): 952-955.
- Ahmad, Absar, et al. "Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum." Colloids and surfaces B: Biointerfaces 28.4 (2003): 313-318.
- Jha, Anal K., and K. Prasad. "PbS nanoparticles: biosynthesis and characterization." International Journal of Nanoparticles 5.4 (2012): 369-379.
- Saxena, J., Sharma, P. K., Sharma, M. M., and Singh, A. Process optimization for green synthesis of silver nanoparticles by Sclerotinia sclerotiorum. Springer Plus5.1 (2016): 1-10.
- Maiti, Swarnali, et al. "Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract." Journal of Analytical Science and Technology 5.1 (2014): 40.
- Nagaich, U., Gulati, N., and Chauhan, S. "Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract." Journal of Pharmaceutics, (2016).
- Shaligram, Nikhil S., et al. "Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain." Process Biochemistry 44.8 (2009): 939-943.
- Alahmad, Abdalrahim, et al. "Preparation of colloidal silver nanoparticles and structural characterization." Physical Sciences Research International 1.4 (2013): 89-96.
- Geetha, A.R., et al. "Optimization of green synthesis of silver nanoparticles from leaf extracts of Pimenta dioica (Allspice)." The Scientific World Journal (2013).
- Madhiyazhagan, Pari, et al. "Sargassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens." Parasitology research 114.11 (2015): 4305-4317.
- Thakkar, K. N., Mhatre, S. S., and Parikh, R. Y. Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine 6.2 (2010): 257-262.
- Hemath Naveen, K.S., et al. “Extracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp.”Arch. Appl. Sci. Res 2.6 (2010):161-167.
- A Green Approach Towards Utilization of Floral Wastes for the Extraction of Natural Colorants
Abstract Views :175 |
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 269-279Abstract
The present study is focused on the utilization of floral wastes for the extraction of natural colorants. Waste flowers were collected from various sites of temples, garden, domestic and decorative areas and identified as Rosahybrid, Tageteserecta, Spathodeacampanulata, Bougainvilleaglabra, and Euphorbiamili. Identification and characterization of extracted colorants was done using thin layer chromatography (TLC), UV Spectroscopy andFourier Transform Infra-red spectroscopy (FT-IR) analysis. Antimicrobial and antioxidant properties of extracted natural colorants were evaluated. Applicability of extracted colorants as natural dye and biofertilizer was also evaluated. It can be concluded that the natural colorants extracted from the floral wastes can be used in textile and pharmaceutical industries.In addition, contamination free natural colorants can also serve as safe biofertilizer.Keywords
Floral Wastes, Natural Colorants, Antimicrobial, Antioxidant, Natural Dye, Biofertilizer.References
- Kulkarni, Bodake, et al. “ Extraction of Natural Dye from Chili (Capsicum Annum) for Textile Coloration”, UJERT, 2011, 1(1)
- Wang, Lin, Chu, et al. “Protective effect of Hibiscus anthocyanins against tertbutylhydroperoxide-induced hepatic toxicity in rats”, Food Chem Toxicol, 2000, 38(5),411-6.
- Kant R,“Textile dyeing industry an environmental hazard”, Natural science, 2012, 4(1), 22-6
- Binapani, Pankaj et al. “Exploration of plant derived natural dyes in Assam”, AJST, 2014, 9(1), 17-20.
- Cronquist A, “The evolution and classification of flowering plants”, The evolution and classification of flowering plants, 1968.
- Mak, Bhat et al. “Antioxidant and antibacterial activities of hibiscus (Hibiscus rosasinensis L.) and Cassia (Senna bicapsularis L.) flower extracts”.JKSUES, 2013, 25(4), 275-82.
- Voon H C, Bhat R et al.“Flower extracts and their essential oils as potential antimicrobial agents for food uses and pharmaceutical applications”, COMPR REV FOOD SCI F., 2012, 11(1):34-55.
- Vankar P S and Srivastava J, “Evaluation of anthocyanin content in red and blue flowers”, IJFE, 2010, 6(4).
- Venugopalan P and K. T, Revathy, “Antioxidant Activity of Cassia Fistula Flower Extracts”. J. Pharm. Appl. Chem., 2016, 2(2), 77-80
- HaddenW L, et al.“Carotenoid composition of marigold (Tagetes erecta) flower extract used as nutritional supplement”, J Agric Food Chem, 1999, 47(10), 4189-94.
- Kamurthy H andDontha S, “Phytochemical Screening on Euphorbia milii Red Flowers â Isolation of Terpenoids, Flavone and Phenols”.AJEthno, 2015, 2(6)
- Singh R and Srivastava S, “Exploration of flower based natural dyes-a review”, Research Journal of Recent Sciences, 2015, (4) 68.
- Sumathi R and Anuradha R, “FT-IR Spectroscopic Studies on Flowers of Allamandaneriifolia” Hook. Int. J. Curr. Microbiol. App. Sci. 2016, 5(6):287-91
- Neha S and Jyoti S, “Phytochemical Analysis of Bougainvillea GlabraChoisy by FTIR and UV-VIS Spectroscopic Analysis”, Int. J. Pharm. Sci. Rev. Res., 2013, (33): 96-198.
- Chakraborty D D, Ravi V, Chakraborty P, “Phytochemical evaluation and TLC protocol of various extracts of Bombaxceiba Linn.” IJPSR, 2010, 1(8):66-73.
- Patil V V, Patil S B et al. “Study of methanolic extract of flower of Spathodea campanulata L. as an anti-solar”, IJGP, 2009, 3 (3).
- Nithyadevi J and Sivakumar R, “Phytochemical screening and GC–MS, FT-IR analysis of methanolic extract leaves of Solanum torvum” Sw. Int. J. Res. Studies in Biosci. 2015, 3(9), 61-6
- Maiti S, Krishnan D, et al. “Antimicrobial activities of silver nanoparticles synthesized from Lycopersiconesculentum extract”, JAST, 2014, 5(1), 40.
- Jain N, “Extraction and application of Natural dye by utilizing temple floral waste Tegeteserecta. L”., IJETSR, 2017, 4(3)
- Grover N, and Vidya P, “Extraction and application of natural dye preparations from the floral parts of Woodfordiafruticosa (Linn.)”Kurz, 2011, IJNPR, 2011, 2(4):403-408
- Bindhu K B, “Effect of Azolla extract on growth performance of Pisum sativum”, IRJBS, 2013, 2(10), 88-90.
- Takhtajan and Armen L, Outline of the classification of flowering plants (Magnoliophyta), The botanical review, (1980), 46(3), 225359.
- Navarro-González I, González-Barrio R, et al. “Nutritional composition and antioxidant capacity in edible flowers: characterization of phenolic compounds by HPLC-DADESI/ MSn”, Int J Mol Sci, 2014, 16(1), 805-22.
- Domenici V, Ancora et al. “Extraction of pigment information from near-UV vis absorption spectra of extra virgin olive oils”, J Agric Food Chem, 2014, 62(38), 9317-25.
- Shurvell H F, “Spectra-structure correlations in the mid- and farinfrared. In: Chalmers, J.M., Griffiths, P.R. (Eds.)”, Handbook of Vibration Spectroscopy. John Wiley and Sons Ltd., UK, 2002, 1783–1816.
- Tejado A, Penaa, et al.“Physico-chemical characterization of lignins from different sources for use in phenol-formaldehyde resin synthesis”, Bioresour Techno, 2007, 98, 1655–1663.