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Wardhani, Puspa
- Comparison of the Effectiveness of Alcohol-based Handrub with the Combination of Chlorhexidine Gluconate, N-propanol and Hydrogen Peroxide in Dr. Soetomo General Hospital, Surabaya, Indonesia
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Authors
Affiliations
1 Resident, Clinical Pathology Specialization Programme, Department of Clinical Pathology, Faculty of Medicine, Airlangga University - Dr. Soetomo Hospital, Surabaya,, ID
2 Department of Clinical Pathology, Faculty of Medicine, Airlangga University - Dr. Soetomo Hospital, Surabaya,, ID
1 Resident, Clinical Pathology Specialization Programme, Department of Clinical Pathology, Faculty of Medicine, Airlangga University - Dr. Soetomo Hospital, Surabaya,, ID
2 Department of Clinical Pathology, Faculty of Medicine, Airlangga University - Dr. Soetomo Hospital, Surabaya,, ID
Source
Research Journal of Pharmacy and Technology, Vol 15, No 3 (2022), Pagination: 1037-1041Abstract
This study aims to analyze the ratio of the number of microorganism colonies on the surface of medical personnel’s hands before and after the use of three types of alcohol-based handrubs. This study is an experimental study with pre and post design at Dr. Soetomo Hospital in September-October 2019 with 20 respondents for each handrub used. T-test was performed to analyze the number of microbe colonies before and after the use of handrub with the combination of chlorhexidine gluconate, meanwhile Wilcoxon test was performed to analyze the number of microbe colonies before and after the use of handrub with the combination of n-propanol and hydrogen peroxide. Effectiveness analysis of the three handrubs was done using Kruskal Wallis test. There was a significant difference in the number of microbes before and after the use of handrub (p 0.05). Hands are an intermediary medium that often transmit pathogenic microorganisms that may cause HAIs. According to WHO, the usage of the three types of alcohol-based handrubs in this research can significantly decrease the number of germs. In conclusion, there was a significant difference in the number of microbial colonies before and after handrub usage. Alcohol-based handrub with the combination of chlorhexidine gluconate had the greatest effect on decreasing microbial colony count in this study. The contents of the three types of handrubs studied in this research were equally effective in decreasing microbial colony numbers on the hands of health workers.Keywords
Healthcare acquired infections, Hand hygiene, Handrub.
References
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- Comparison of Diagnostic Tests for Detection of Nonstructural-1(Ns1) Antigen Dengue Virus Using Immunochromatography and Fluorescence Immunoassay Methods
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Authors
Nelly Zuroidah
1,
May Fanny Tanzilia
2,
I Gusti Agung Ayu Eka Putri Sunari
1,
Billy Jordan Wrahatnala
3,
Faradila Khoirun Nisa Haki
3,
Aryati
4,
Puspa Wardhani
5,
Dominicus Husada
6,
Ali Rohman
7,
Siti Nadia Tarmizi
8
Affiliations
1 Clinical Pathology Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
2 Clinical Pathology Sub-Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
3 Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
4 Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
5 Institute of Tropical Disease, Universitas Airlangga, Surabaya, ID
6 6Department of Pediatric, Faculty of Medicine – Universitas Airlangga, Surabaya., ID
7 Department of Chemistry, Faculty of Science and Technology – Universitas Airlangga, Surabaya., ID
8 Directorate of Vector Borne and Zoonotic Disease, Ministry of Health Republic of Indonesia, Jakarta,, ID
1 Clinical Pathology Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
2 Clinical Pathology Sub-Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
3 Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
4 Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, ID
5 Institute of Tropical Disease, Universitas Airlangga, Surabaya, ID
6 6Department of Pediatric, Faculty of Medicine – Universitas Airlangga, Surabaya., ID
7 Department of Chemistry, Faculty of Science and Technology – Universitas Airlangga, Surabaya., ID
8 Directorate of Vector Borne and Zoonotic Disease, Ministry of Health Republic of Indonesia, Jakarta,, ID
Source
Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1494-1498Abstract
Background : NS1 is currently widely used for diagnosis of dengue virus (DENV) infection. Various methods are used to diagnose DENV infection (DVI), either ELISA, immunochromatography (ICT) or most recently the fluorescence immunoassay (FIA) method which are commercially available. Objective: This study aimed to compare the detection capabilities of dengue NS1 antigens using (1) Dengue NS1 ICT Ag (Standard Q - SD Biosensor, Inc.), (2) Dengue NS1 ICT Ag (SD Bioline - Standard Diagnostic, Inc), and (3) Dengue NS1 Ag FIA (Standard F - SD Biosensor, Inc.) Methods: This study consisted of serum samples (n=80) with the number of DVI patients (n=50), non-DVI (n=30). All samples were examined using all three commercial kits for NS1 antigen testing. All DVI samples showed results of reverse-transcriptase polymerase chain reaction (RT-PCR - SIMPLEXAᵀᴹ Dengue - Focus Diagnostics) and/or positive dengue NS1 (Panbio® Dengue Early ELISA) antigen. Results: Standard F showed the highest sensitivity (82%) compared to Standard Q (74%) and SD Bio line (74%). These three commercial kits had the same specificity 100%. The positive predictive value all of these kits was 100% each. The negative prediction value of Standard F, Standard Q, and SD Bio line were 76.9%, 63.8%, 63.8%, respectively. These three NS1 antigen tests had a good agreement (κ 0.681-0.774). Conclusions: FIA test performance (Standard F SD - Biosensor, Inc.) were a quick and easy examination, showing a higher sensitivity and specificity than ICT for detecting DENV infection. Further research is needed to confirm the diagnosis of primary or secondary infection.Keywords
Dengue Viral Infection, NS1 Dengue antigen, ICT, FIA.References
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