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Dhanalakshmi, S.
- In Vitro Anti-Oxidant Study of Herbal Extract Mixture by Nitric oxide and DPPH Method
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Affiliations
1 Department of Pharmacognosy, School of Pharmaceutical Sciences, VISTAS, Vels University, Pallavaram, Chennai-117, Tamilnadu, IN
1 Department of Pharmacognosy, School of Pharmaceutical Sciences, VISTAS, Vels University, Pallavaram, Chennai-117, Tamilnadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 1 (2017), Pagination: 277-280Abstract
Herbalism is a traditional medicine or folk medicine practice based on the use of plants or it's extracts. Herbal extract mixture produce synergetic activity compared to individuals, it was confirmed by its in- vitro antioxidant activity. Herb-herb combinations have been used in practice for thousands of years, yet scientific evidence of their therapeutic benefits is lacking.An increase demand for this , the present work examine thepotential of Herbal extract mixture. Mixtures were prepared in different combination (aqueous extract of Terminalia belerica : aqueous extract of Solanum xanthocarpum) ratio of 10:90, 20:80, 30 :70,40:60,50:50,60:40,70:30,80:20,90:10. All ratios were subjected to nitric oxide scavenging assay to find out the effective combinational mixture. The ratio 70:30 (Terminalia belerica : Solanum xanthocarpum ) showed maximum free radical scavenging property. The present results suggest that traditional crude drugs might be potent and novel therapeutic agents for scavenging of NO and the regulation of pathological conditions caused by excessive NO and its oxidation product. These findings may also help to explain, at least in part, certain pharmacological activities of crude drugs, especially anti-infection and anti-inflammatory activities.Keywords
Solanum Xanthocarpum (entire plant ), Terminalia Belerica (fruit) , Herbal Extract Mixture Preparation. Tannin and Nitric Oxide Free Radical Scavenging Assay, DPPH Method.
- Development and Optimization of Natural Colourant from Microbial Pigment
Abstract Views :316 |
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Authors
Affiliations
1 Dr. M. G. R Educational and Research Institute, Velappanchavadi, Chennai – 600077, Tamil Nadu, IN
1 Dr. M. G. R Educational and Research Institute, Velappanchavadi, Chennai – 600077, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3475-3478Abstract
Background: Natural pigments and synthetic dyes have been extensively used in various field such as food production, textile industries etc. Prodigiosin is red pigment produced by Serratia species. Prodigiosin is a promising drug owing to its reported characteristics of having anti-microbial, immunosuppressive, anti-proliferative and anti-cancer activity. Objective: The objective of the study is to development and optimization of natural colourant from microbial pigment. Methods: The aim of the work is to formulate suitable growth conditions and interaction of carbon and nitrogen sources in medium for growth production of prodigiosin from Serratia species. Result: The maximum production A2 (units/cell) was achieved at 280C, at pH7 with the addition of 1% of carbon source (Glucose) and 0.25% of nitrogen sources (peptone)after 72 days of incubation. Conclusion: Our results indicate that we have designed an economic medium supporting enhanced Serratia marcescens A2 prodigiosin production giving an added value for crude glycerol obtained from biodiesel industry.Keywords
Serratia, Bio Pigment, Factorial Design, Prodigiosin.References
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