Research Journal of Pharmacy and Technology

S. Jayakumari ¹, V. Ravichandiran ²

Affiliations
1 Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels University (VISTAS) Chennai, Tamilnadu, IN
2 NIPER, Kolkotta, IN

Abstract

Cyclooxygenase-2 (COX-2) inhibition is an important approach in the management of inflammatory problems. Since synthetic compounds may exaggerate the levels of inflammatory mediators via COX pathway, so many researchers are focusing in search of natural compound or bioguided fraction for COX-2 inhibitors. From the literature review it is evident that natural polyphenols such as flavonoid and tannins have been found exert its anti-inflammatory effects either by decreasing formation of reactive oxygen species or by modulation of proinflammatory gene expression of Cyclooxygenase by acting through Nuclear factor - kappa B (NF-kappa) and Mitogen Activated Protein Kinase signaling. Earlier literature revealed that Terminalia chebula (Fruits); Sida rhombifolia (Root) Leucas cephalotes (Entire plant) were found to contain polyphenolic compounds and steroid and are used in Indian traditional system of medicine for inflammatory associated problems. The earlier scientific reports showed the extract of the above plants have significant anti-inflammatory activity in animal model. Hence the present study is focused to separate the fraction from the selected plants and study the effect of combined fractions for inhibition of inflammatory key enzymes .Further the bioactive fraction was standardized by HPTLC as per standard protocol. The outcome of the present work would pave a pathway for finding a new phytopharmacophore from Indian meditational plants for treatment of inflammatory associated diseases.

Keywords

Terminalia chebula fruits, Sida rhombifolia ischolar_main, Leucas cephalotes Entire Plant. Tannin, Steroid, Flavonoid Fractions and COX- II Assay.

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A. Vijayalakshmi ¹, M. Suganya ¹, Pankaj Kumar Giri ¹, S. Jayakumari ¹, P. Shanmugasundaram ¹

Affiliations
1 Department of Pharmacognosy, School of Pharmaceutical Sciences, VISTAS, Vels University, Pallavaram, Chennai-117, Tamilnadu, IN

Abstract

The present study aimed to evaluate anti-asthmatic activity of a classical Siddha formulation Seenthil chooranam (SC) by mast cell stabilizing effect and broncho dilator property by in-vivo method. Preliminary phytochemical and HPTLC analysis of SC were determined as per standard protocols. Phytochemical analysis of aqueous extract gave positive test for carbohydrates, phenols, glycosides, saponins, flavonoids, tannins and terpenoids. HPTLC finger print analysis of the aqueous extract showed the presence of possible number of components. The results of the in-vivo study demonstrate that SC has potent broncho dilator property with significant (p<0.001) mast cell stabilizing activity and decrease in leukocytosis in dose dependent manner. These findings are clearly indicative of the role of SC as potent inhibitor of mast cell degranulation and ability to control the leukocytosis, have bronchodilation and hence can be used for the management of asthma supporting the traditional claims.

Keywords

Seenthil Chooranam, Siddha Formulation, Asthma, Mast Cell.

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V. P. Laavanya ¹, R. Jayasurya ¹, N. Kavitha ¹, G. Prabakaran ¹, S. Jayakumari ¹

Affiliations
1 School of Pharmaceutical Sciences, Vels University, Vels Institute of Science, Technology and Advanced Studies [VISTAS] Pallavaram, Chennai, 600 117, Tamilnadu, IN

Abstract

A widespread epidemic of Zika virus (ZIKV) infection was reported in 2015 in South and Central America and the Caribbean. A major concern associated with this infection is the apparent increased incidence of microcephaly in fetuses born to mothers infected with(ZIKV). In this report, it was described the case of an expectant mother who had a febrile illness with rash at the end of the first trimester of pregnancy living in Brazil. Ultrasonography performed at 29 weeks of gestation revealed microcephaly with calcifications in the fetal brain and placenta. After the mother requested termination of the pregnancy, a fetal autopsy was performed. Microcephaly (an abnormally small brain) was observed, with almost complete agyria, hydrocephalus, and multifocal dystrophic calcifications in the cortex and subcortical white matter, with associated cortical displacement and mild focal inflammation. ZIKV was found in the fetal brain tissue on reverse-transcriptase- polymerase-chain-reaction (RT-PCR) assay, with consistent findings on electron microscopy. The complete genome of (ZIKV) was recovered from the fetal brain.

Keywords

Zika Virus (flavivirus) Uganda, Microcephaly, Reverse-Transcriptase–Polymerase-Chain-Reaction (RT-PCR).

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