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Amin Rafeeqi, Towseef
- Effect of Anti-inflammatory Activity of Aqueous, Hydro-ethanol and Methanol Extracts of Two Unani Formulations
Abstract Views :390 |
PDF Views:0
Authors
Lahari Kuna
1,
Shravan Kumar Ghali
1,
Towseef Amin Rafeeqi
2,
Gulam Mohammed Husain
1,
Mohammed Abdul Waheed
1,
Ghazala Javed
3,
Munawwar Husain Kazmi
1,
Alokananda Chakraborty
1
Affiliations
1 National Research Institute of Unani Medicine for Skin Disorders (NRIUM-SD), ESI-X-Road, Hyderabad - 500038, Telangana, IN
2 Regional Research Institute of Unani Medicine Balasore - Bhadrak - Cuttack Rd, Bankashi, Bhadrak, Odisha 756100., IN
3 Central Council for Research in Unani Medicine 61-65, opp. D-Block, Janakpuri Institutional Area, Janakpuri, New Delhi, Delhi 110058., IN
1 National Research Institute of Unani Medicine for Skin Disorders (NRIUM-SD), ESI-X-Road, Hyderabad - 500038, Telangana, IN
2 Regional Research Institute of Unani Medicine Balasore - Bhadrak - Cuttack Rd, Bankashi, Bhadrak, Odisha 756100., IN
3 Central Council for Research in Unani Medicine 61-65, opp. D-Block, Janakpuri Institutional Area, Janakpuri, New Delhi, Delhi 110058., IN
Source
Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1560-1566Abstract
In view inflammation causes of development of some chronic diseases like rheumatoid arthritis, obesity, diabetes, asthma. Cell model in vitro in an effort to provide an understanding about the cellular and molecular mechanism of Unani formulation are being used for treatment of various inflammatory diseases including arthritis for centuries. Method Antioxidant by DPPH assay. Cell viability through MTT assay. Measurement of NO level and ROS levels by (DCFDA) in RAW cells. Results in DPPH Assay showed all the extracts ofUNIM-302 shows good antioxidant activity when compared to UNIM301. UNIM drugs show no substantive cytotoxic activity against RAW macrophages cell line. UNIM301, UNIM 302 inhibited the production of Nitric oxide production in RAW cells. ROS the results found that UNIM301 and UNIM302 of all extracts could significantly inhibits the LPS stimulation and reduces production of ROS in RAW cells. In conclusion study demonstrated that both Unani formulations inhibits NO and ROS production in LPS induced RAW macrophages. Activity in all different extracts UNIM 302 exhibited better antioxidant compared to UNIM 301these unim formulation extracts can be used as natural sources of antioxidants potent anti inflammatory agent and exhibits inflammatory preventive properties.Keywords
Antioxidant, UNIM Medicine, RAW cells, UNIM 301, UNIM302, Macrophages.
References
- Yu et al., The pivotal role TBK1 in inflammatory responses mediated by macrophages. Mediators of Inflammation. 2012; 979105.
- Blazovics et al., Cytokines, prostaglandins, nutritive and non-Nutritive factors in inflammatory bowel diseases. Orvosi Hetilap. 2004; 145, 2523–2529.
- Huo et al., Anti-inflammatory effects of linalool in RAW264.7 macrophages and lip polysaccharide-induced lung injury model. Journal of Surgical Research. 2012; 180, e47–e54.
- Vargas et al., Ragweed pollen extract intensifies lip polysaccharide-induced priming of NLRP3 inflam-masome in human macrophages. Immunology. 2013; 138,392–401.
- Liu and Mali. NF-kB activation as a pathological mechanism of septic shock and inflammation. Am. J. Physiol. Lung Cell Mol. Physiol. 2006; 290, L622–L645.
- Jue et al., Nuclear factor κB (NF-κB) pathway as a therapeutic target in rheumatoid arthritis. J Korean Med Sci. 1999; 14(3):231-238.
- Nabarun Mukhopadhyay, Sampath. V, Sameer Pai, U. V. Babu, Richard Lobo. Antiarthritic Medicinal Plants: A Review. Research J. Pharm. and Tech. 2019; 12(1): 375-381. doi: 10.5958/0974-360X.2019.00068.4
- AYUSH Department Unani system of medicine the science of health and healing. Ministry of Health & FW. (2013); Govt of India.
- Siddiqui KM, Parveen S, Jamil SS. Multicentric observational studies of polyherbalUnani oral and local formulations in cases of rheumatoid arthritis. Planta Med. 2011; 77:149.
- Youwei Z, Jinlian Z, Yonghong P .A comparative study on the free radical scavengingactivities of some fresh flowers in southern China. LWT- Food Science and Technology.2008;41:1586-1591.
- Mariathasan S, Monack DM. Inflammasome adaptors andsensors: intracellular regulators of infection and inflammation. Nat Rev Immunol. 2007; 7: 31–40.
- Duffield JS. The inflammatory macrophage: a story of Jekylland Hyde. Clin Sci. 2003;104: 27–38.
- Heiss E, Herhaus C, Klimo K, Bartsch H, Gerhauser C. Nuclearfactor kappa B is a molecular target for sulforaphane mediated anti-inflammatory mechanisms. J Biol Chem. 2001; 276:32008–32015.
- M. A. Ebrahimzadeh, S. M. Nabavi, S. F. Nabavi, F. Bahramian, and A. R. Bekhradnia. Antioxidant and free radical scavengingactivity of H. officinalis L. var. angustifolius, V. odorata, B.hyrcana and C. speciosum. Pakistan Journal of Pharmaceutical Sciences. 2010; vol. 23, no. 1, pp. 29–34.
- A. A. Adedapo, F. O. Jimoh, S. Koduru, P. J. Masika, and A.J. Afolayan, “Assessment of the medicinal potentials of themethanol extracts of the leaves and stems of Buddleja saligna, ”BMC Complementary and Alternative Medicine. 2009. Vol. 9, Article 21.
- Geetha S, Sai-Ram M, Mongia SS, Singh V, Ilavazhagan G, et al Evaluation ofantioxidant activity of leaf extract of Sea buckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J Ethnopharmacol. 2003; 87:247–251.
- Ch. Madhu, J. Swapna, K. Neelima, Monic V. Shah. A Comparative Evaluation of the Antioxidant Activity of Some Medicinal Plants Popularly Used in India. Asian J. Res. Pharm.Sci. 2(3): July-Sept. 2012; Page 98-100
- Swahili F Natural antioxidants: An overview. In Natural Antioxidants, Chemistry, Health Effects and Applications. Shahidi F (ed) Champaign: 1997; AOCS Press; 1-11.
- Kalim MD, Bhattacharyya D, Banerjee A, Chattopadhyay S Oxidative DNA damagepreventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complement Altern Med. 2010; 10:77 .
- Munhoz CD, Garcia-Bueno B, Madrigal JLM, Lepsch LB,Scavone C, Leza JC.. Stress-induced neuroinflammation: mechanisms and new pharmacological targets. Bruz J Med Biol Res. 2008; 41: 1037-1046.
- Patel D.S., Shah P. B., Managoli N. B.. Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian J. Pharm. Tech. 2(4): Oct. - Dec. 2012; Page 143-147.
- Sudhanshu Kumar Meher, Banmali Das, Purnendu Panda, GC Bhuyan, MM Rao. Uses of Withania somnifera (Linn) Dunal (Ashwagandha) in Ayurveda and its Pharmacological Evidences. Research Journal of Pharmacology and Pharmacodynamics. 2016; 8(1): January-March, 23-29. doi: 10.5958/2321-5836.2016.00006.9.
- T. Jeyanthi, P. Subramanian, P. Kumaravel. A Comparative Analysis of Antibacterial Activity of Withania somnifera Root Extract with Commercial Antibiotics. Asian J. Pharm. Res. 3(2): April- June 2013; Page 98-102.
- Shweta Singh Chauhan, Pramod Kumar Mahish. Flavonoids of the flame of forest- Butea monosperma. Research J. Pharm. and Tech. 2020; 13(11):5647-5653. doi: 10.5958/0974-360X.2020.00984.1
- Nilesh Gupta, U.K Jain, Ankit Jain, Goutam Lovanshi, Nitin Mathan, Vipin Tiwari. Review of Some Important Medicinal Plants Possesses Anti-Inflammatory Activity. Research J. Pharm. and Tech. 4(10): Oct. 2011; Page 1506-1512.
- Choi MS, Lee SH, Cho HS, et al. Inhibitory effect of obovatol on nitric oxide production and activationof NF-kappaB/MAP kinases in lipopolysaccharide-treated RAW 264.7cells. Eur J Pharmacol. 2007; 556:181–189.
- Natarajan Bhaskaran, Sanjeev Shukla, Janmejai K Srivastava, and Sanjay Gupta Chamomile, an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking Rel A/p65 activity. Int J Mol Med. 2010; December ; 26(6): 935–940.
- Paola Rosanna D, Salvatore C. Reactive oxygen species, inflammation, and lung diseases. Curr Pharm Des. 2012; 18(26):3889±900.
- Zhang J, Wang X, Vikash V, Ye Q, Wu D, Liu Y, et al. ROS and ROS-Mediated Cellular Signaling. OxidMed Cell Longev. 2016; 2016:4350965. PMCID: PMC4779832.
- Hörmann HP, Korting HC. Evidence for the efficacy and safety of topical herbal drugs in dermatology: Part I: Anti-inflammatory agents. Phytomedicine. 1994; 1:161–71.
- Effect of Anti-inflammatory Activity of Aqueous, Hydro-ethanol and Methanol Extracts of Two Unani Formulations
Abstract Views :145 |
PDF Views:0
Authors
Lahari Kuna
1,
Shravan, Kumar Ghali
1,
Towseef Amin Rafeeqi
2,
Gulam Mohammed Husain
1,
Mohammed Abdul Waheed
1,
Ghazala Javed
3,
Munawwar Husain Kazmi
1,
Alokananda Chakraborty
1
Affiliations
1 National Research Institute of Unani Medicine for Skin Disorders (NRIUM-SD), ESI-X-Road, Hyderabad - 500038, Telangana,, IN
2 RRIUM,Srinagar ,Jammu and Kashmir., IN
3 Central Council for Research in Unani Medicine 61-65, opp. D-Block, Janakpuri Institutional Area, Janakpuri, New Delhi, Delhi 110058., IN
1 National Research Institute of Unani Medicine for Skin Disorders (NRIUM-SD), ESI-X-Road, Hyderabad - 500038, Telangana,, IN
2 RRIUM,Srinagar ,Jammu and Kashmir., IN
3 Central Council for Research in Unani Medicine 61-65, opp. D-Block, Janakpuri Institutional Area, Janakpuri, New Delhi, Delhi 110058., IN
Source
Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1560-1566Abstract
In view inflammation causes of development of some chronic diseases like rheumatoid arthritis, obesity, diabetes, asthma. Cell model in vitro in an effort to provide an understanding about the cellular and molecular mechanism of Unani formulation are being used for treatment of various inflammatory diseases including arthritis for centuries. Method Antioxidant by DPPH assay. Cell viability through MTT assay. Measurement of NO level and ROS levels by (DCFDA) in RAW cells. Results in DPPH Assay showed all the extracts ofUNIM-302 shows good antioxidant activity when compared to UNIM301. UNIM drugs show no substantive cytotoxic activity against RAW macrophages cell line. UNIM301, UNIM 302 inhibited the production of Nitric oxide production in RAW cells. ROS the results found that UNIM301 and UNIM302 of all extracts could significantly inhibits the LPS stimulation and reduces production of ROS in RAW cells. In conclusion study demonstrated that both Unani formulations inhibits NO and ROS production in LPS induced RAW macrophages. Activity in all different extracts UNIM 302 exhibited better antioxidant compared to UNIM 301these unim formulation extracts can be used as natural sources of antioxidants potent anti inflammatory agent and exhibits inflammatory preventive properties.Keywords
Antioxidant, UNIM Medicine, RAW cells, UNIM 301, UNIM302, Macrophages.References
- Yu et al., The pivotal role TBK1 in inflammatory responses mediated by macrophages. Mediators of Inflammation. 2012; 979105.
- Blazovics et al., Cytokines, prostaglandins, nutritive and non-Nutritive factors in inflammatory bowel diseases. Orvosi Hetilap. 2004; 145, 2523–2529.
- Huo et al., Anti-inflammatory effects of linalool in RAW264.7 macrophages and lip polysaccharide-induced lung injury model. Journal of Surgical Research. 2012; 180, e47–e54.
- Vargas et al., Ragweed pollen extract intensifies lip polysaccharide-induced priming of NLRP3 inflam-masome in human macrophages. Immunology. 2013; 138,392–401.
- Liu and Mali. NF-kB activation as a pathological mechanism of septic shock and inflammation. Am. J. Physiol. Lung Cell Mol. Physiol. 2006; 290, L622–L645.
- Jue et al., Nuclear factor κB (NF-κB) pathway as a therapeutic target in rheumatoid arthritis. J Korean Med Sci. 1999; 14(3):231-238.
- Nabarun Mukhopadhyay, Sampath. V, Sameer Pai, U. V. Babu, Richard Lobo. Antiarthritic Medicinal Plants: A Review. Research J. Pharm. and Tech. 2019; 12(1): 375-381. doi: 10.5958/0974-360X.2019.00068.4
- AYUSH Department Unani system of medicine the science of health and healing. Ministry of Health & FW. (2013); Govt of India.
- Siddiqui KM, Parveen S, Jamil SS. Multicentric observational studies of polyherbalUnani oral and local formulations in cases of rheumatoid arthritis. Planta Med. 2011; 77:149.
- Youwei Z, Jinlian Z, Yonghong P .A comparative study on the free radical scavengingactivities of some fresh flowers in southern China. LWT- Food Science and Technology.2008;41:1586-1591.
- Mariathasan S, Monack DM. Inflammasome adaptors andsensors: intracellular regulators of infection and inflammation. Nat Rev Immunol. 2007; 7: 31–40.
- Duffield JS. The inflammatory macrophage: a story of Jekylland Hyde. Clin Sci. 2003;104: 27–38.
- Heiss E, Herhaus C, Klimo K, Bartsch H, Gerhauser C. Nuclearfactor kappa B is a molecular target for sulforaphane mediated anti-inflammatory mechanisms. J Biol Chem. 2001; 276:32008–32015.
- M. A. Ebrahimzadeh, S. M. Nabavi, S. F. Nabavi, F. Bahramian, and A. R. Bekhradnia. Antioxidant and free radical scavengingactivity of H. officinalis L. var. angustifolius, V. odorata, B.hyrcana and C. speciosum. Pakistan Journal of Pharmaceutical Sciences. 2010; vol. 23, no. 1, pp. 29–34.
- A. A. Adedapo, F. O. Jimoh, S. Koduru, P. J. Masika, and A.J. Afolayan, “Assessment of the medicinal potentials of themethanol extracts of the leaves and stems of Buddleja saligna, ”BMC Complementary and Alternative Medicine. 2009. Vol. 9, Article 21.
- Geetha S, Sai-Ram M, Mongia SS, Singh V, Ilavazhagan G, et al Evaluation ofantioxidant activity of leaf extract of Sea buckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J Ethnopharmacol. 2003; 87:247–251.
- Ch. Madhu, J. Swapna, K. Neelima, Monic V. Shah. A Comparative Evaluation of the Antioxidant Activity of Some Medicinal Plants Popularly Used in India. Asian J. Res. Pharm. Sci. 2(3): July-Sept. 2012; Page 98-100
- Swahili F Natural antioxidants: An overview. In Natural Antioxidants, Chemistry, Health Effects and Applications. Shahidi F (ed) Champaign: 1997; AOCS Press; 1-11.
- Kalim MD, Bhattacharyya D, Banerjee A, Chattopadhyay S Oxidative DNA damagepreventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complement Altern Med. 2010; 10:77 .
- Munhoz CD, Garcia-Bueno B, Madrigal JLM, Lepsch LB,Scavone C, Leza JC.. Stress-induced neuroinflammation: mechanisms and new pharmacological targets. Bruz J Med Biol Res. 2008; 41: 1037-1046.
- Patel D.S., Shah P. B., Managoli N. B.. Evaluation of In-vitro Anti-oxidant and Free Radical Scavenging activities of Withania somnifera and Aloe vera. Asian J. Pharm. Tech. 2(4): Oct. - Dec. 2012; Page 143-147.
- Sudhanshu Kumar Meher, Banmali Das, Purnendu Panda, GC Bhuyan, MM Rao. Uses of Withania somnifera (Linn) Dunal (Ashwagandha) in Ayurveda and its Pharmacological Evidences. Research Journal of Pharmacology and Pharmacodynamics. 2016; 8(1): January-March, 23-29. doi: 10.5958/2321-5836.2016.00006.9.
- T. Jeyanthi, P. Subramanian, P. Kumaravel. A Comparative Analysis of Antibacterial Activity of Withania somnifera Root Extract with Commercial Antibiotics. Asian J. Pharm. Res. 3(2): April- June 2013; Page 98-102.
- Shweta Singh Chauhan, Pramod Kumar Mahish. Flavonoids of the flame of forest- Butea monosperma. Research J. Pharm. and Tech. 2020; 13(11):5647-5653. doi: 10.5958/0974-360X.2020.00984.1
- Nilesh Gupta, U.K Jain, Ankit Jain, Goutam Lovanshi, Nitin Mathan, Vipin Tiwari. Review of Some Important Medicinal Plants Possesses Anti-Inflammatory Activity. Research J. Pharm. and Tech. 4(10): Oct. 2011; Page 1506-1512.
- Choi MS, Lee SH, Cho HS, et al. Inhibitory effect of obovatol on nitric oxide production and activationof NF-kappaB/MAP kinases in lipopolysaccharide-treated RAW 264.7cells. Eur J Pharmacol. 2007; 556:181–189.
- Natarajan Bhaskaran, Sanjeev Shukla, Janmejai K Srivastava, and Sanjay Gupta Chamomile, an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking Rel A/p65 activity. Int J Mol Med. 2010; December ; 26(6): 935–940.
- Paola Rosanna D, Salvatore C. Reactive oxygen species, inflammation, and lung diseases. Curr Pharm Des. 2012; 18(26):3889±900.
- Zhang J, Wang X, Vikash V, Ye Q, Wu D, Liu Y, et al. ROS and ROS-Mediated Cellular Signaling. OxidMed Cell Longev. 2016; 2016:4350965. PMCID: PMC4779832.
- Hörmann HP, Korting HC. Evidence for the efficacy and safety of topical herbal drugs in dermatology: Part I: Anti-inflammatory agents. Phytomedicine. 1994; 1:161–71.