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A simple and rapid HPTLC analysis was developed for the quantitative determination of diosgenin and solasodine from hydroethanolic extract of Solanum nigrum L. The two variants of Solanum nigrum - black and orange fruits - are used in various parts of the world as food and medicine drastically. These two variants showed distinctive features in morphology and anatomy. To probe the phytoconstituent variation among these two variants, HPTLC analysis was done and the results of present HPTLC analysis showed important differences in peak values. So this study may provide scientific validation on spectral characteristics of two variants of Solanum nigrum L. with black and orange fruits.
Diosgenin, HPTLC, Hydroethanolic Extract, Solanum nigrum, Solanaceae, Solasodine