ScieXplore: International Journal of Research in Science

Open Access Open Access  Restricted Access Subscription Access

Analysis of Genetic Diversity of Plumbago zeylanica L.


Affiliations
1 P.T.M.T.M College, Department of Botany, Kamuthi, Tamilnadu, India
2 St. Xavier’s College (Autonomous), Palayamkottai, Tamilnadu, India
 

Plumbago zeylanica is extensively used in herbal medicines and it has an anticancer compounds. The current investigation examines the genetic diversity of Plumbago zeylanica from five different populations of the Western Ghats using ISSR markers. Twenty primers were screened of which twelve were found to be the most informative. Based on Jaccard's coefficient, the P. zeylanica populations confirm a high level of genetic variability or heterozygosity. The PZ1 (Kallar -Ooty) accession has the highest percentage (70.59 %) of polymorphism. The overall mean value of genetic diversity (HT) in the group is 0.43 whereas the diversity within populations (Hs) is 0.30. The overall locus of Genetic differentiation (GST) between populations is 0.30. Inter population gene flow (Nm = 1.28) shows that the genetic exchange among populations is limited. Thus the ISSR marker is useful to study the genetic diversity of Plumbago populations.

Keywords

Genetic Diversity, Inter Simple Sequence Repeats (ISSR), Plumbago zeylanica L.
User
Notifications
Font Size


  • Bousquet J., Simon L., Lalonde M., “DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction”, Can J For Res, Vol. 20, pp. 254–457, 1990.

  • Deeksha B., Anil S., Mukesh K., Vipin K., Sunil M., Shiveta S., Shailendra S., “Comparative genetic diversity analysis in chrysanthemum: A pilot study based on morpho-agronomic traits and ISSR markers”, Sci Horticult, Vol. 167(6), pp. 164–168, 2014.

  • Dharmar K., John De Britto A., “DNA fingerprinting of Plumbago zeylanica L. using ISSR Markers”, Int J App Biores, Vol. 6, pp. 23–27, 2012.

  • Fei L., Qiao-sheng G., Hong-zhuan S., Fu L., Ye-bing Y., Lin-lan L., Jin-tian H., Ai-ming W., Hui-xing L., “Genetic diversity analysis of Perinereis aibuhitensis based on ISSR and SRAP markers of Chinese coast populations”, Biochem Syst Ecol, Vol. 57, pp. 262–269, 2014.

  • Liyuan C., Faju C., Suichao H., Luyi M., “High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP markers”, Elect J Biotech, Vol. 1(6), pp. 268–274, 2014.

  • Rohlf R. J., NTSYS-PC. “Numerical taxonomy and multivariate analysis system, version, 1.50”, Exeter Publication Ltd: New York; 1989.

  • Shikha S., Manoj K. P., Sanghamitra N., “Evaluation of genetic diversity in turmeric (Curcuma longa L.) using RAPD and ISSR markers”, Ind Crops Prod. Vol. 37(1), pp. 284–291, 2012.

  • Sneath P. H. A., Sokal R. R., “Numerical Taxonomy”, WH Freeman and Company: San Francisco; 1973.

  • Umadevi M., Sampath K., Debjit B. K. P., Duraivel S., “Traditionally used Anticancer Herbs In India”. Journal of Medicinal Plants, Vol. 1(3), pp. 56–74, 2013.

  • Ying L., Ju-ming Z., Xian-guo W., Fang L., Zhong-bao S., “Genetic diversity in Vicia amoena (Fabaceae) germplasm resource in China using SRAP and ISSR Markers”, Biochem Syst and Ecol, Vol. 51, pp. 86–93, 2013.

  • Zhiyong W., Li L., Xuejun Y., Hailin G., Aigui G., Jianxiu L., “Genetic diversity analysis of Cynodon dactylon (bermudagrass) accessions and cultivars from different countries based on ISSR and SSR Markers”, Biochem Syst Ecol. Vol. 46, pp. 108–115, 2013.


Abstract Views: 726

PDF Views: 337




  • Analysis of Genetic Diversity of Plumbago zeylanica L.

Abstract Views: 726  |  PDF Views: 337

Authors

K. Dharmar
P.T.M.T.M College, Department of Botany, Kamuthi, Tamilnadu, India
A. John De Britto
St. Xavier’s College (Autonomous), Palayamkottai, Tamilnadu, India

Abstract


Plumbago zeylanica is extensively used in herbal medicines and it has an anticancer compounds. The current investigation examines the genetic diversity of Plumbago zeylanica from five different populations of the Western Ghats using ISSR markers. Twenty primers were screened of which twelve were found to be the most informative. Based on Jaccard's coefficient, the P. zeylanica populations confirm a high level of genetic variability or heterozygosity. The PZ1 (Kallar -Ooty) accession has the highest percentage (70.59 %) of polymorphism. The overall mean value of genetic diversity (HT) in the group is 0.43 whereas the diversity within populations (Hs) is 0.30. The overall locus of Genetic differentiation (GST) between populations is 0.30. Inter population gene flow (Nm = 1.28) shows that the genetic exchange among populations is limited. Thus the ISSR marker is useful to study the genetic diversity of Plumbago populations.

Keywords


Genetic Diversity, Inter Simple Sequence Repeats (ISSR), Plumbago zeylanica L.

References





DOI: https://doi.org/10.15613/sijrs%2F2015%2Fv2i1%2F80081