Toxicology International (Formerly Indian Journal of Toxicology)

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Asessment of the Hepatoprotective and Antioxidant Effect of Aqueous Leaf Extract of Costus after “Ker Gawl” on Cyclosporine a Induced Hepatotoxicity


Affiliations
1 Department of Experimental Pharmacology and Toxicology, University of Port Harcourt, Rivers State, Nigeria
     

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Objective: Costus afer has long been used in traditional African setting in several instances though lack a prove of evidence for its numerous acclaimed potentials, however, this study is designed to investigate the hepatoprotective and antioxidant properties of aqueous leaf extract of C. afer against cyclosporine A (CsA) induced hepatotoxicity. Methods: Rats of similar weight were divided into six groups of five rats each. Groups 1 and 2 served as normal and toxic control received olive oil and 50 mg/kg CsA p.o., respectively. Groups 3, 4, 5, and 6 were given 375, 750, 1125 mg/kg p.o of the extract, and 100 mg/kg silymarin an hour prior to 50 mg/kg CsA, respectively, for 5 days. Physical, biochemical, and histological parameters estimated by reported methods serves as scientific evidence in the study. Results: C. afer significantly restored catalase (P < 0.005), superoxide dismutase (P < 0.005), malondialdehyde (P < 0.005), and glutathione (P < 0.05) levels dose dependently. In all cases as mentioned previously, C. afer at all dose level promoted significant (P < 0.05) restoration of serum liver parameters such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, conjugated bilirubin, total bilirubin, total protein, and albumin. The biochemical observations were supplemented with histopathological reports, which showed attenuation of hepatocellular necrosis. Conclusion: Aqueous leaf extract of C. afer dose dependently prevented the significant changes on the physical, biochemical, and histological changes induced by CsA in the liver. Therefore, aqueous leaf extract of C. afer can be employed as an economic agent to treat liver diseases related to CsA administration and possibly other hepatotoxic drugs.

Keywords

Costus afer, Hepatocellular Necrosis, Liver Enzyme, Oxidative Stress, Silymarin.
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  • Asessment of the Hepatoprotective and Antioxidant Effect of Aqueous Leaf Extract of Costus after “Ker Gawl” on Cyclosporine a Induced Hepatotoxicity

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Authors

Anthonet Ndidiamaka Ezejiofor
Department of Experimental Pharmacology and Toxicology, University of Port Harcourt, Rivers State, Nigeria
Orish Ebere Orisakwe
Department of Experimental Pharmacology and Toxicology, University of Port Harcourt, Rivers State, Nigeria

Abstract


Objective: Costus afer has long been used in traditional African setting in several instances though lack a prove of evidence for its numerous acclaimed potentials, however, this study is designed to investigate the hepatoprotective and antioxidant properties of aqueous leaf extract of C. afer against cyclosporine A (CsA) induced hepatotoxicity. Methods: Rats of similar weight were divided into six groups of five rats each. Groups 1 and 2 served as normal and toxic control received olive oil and 50 mg/kg CsA p.o., respectively. Groups 3, 4, 5, and 6 were given 375, 750, 1125 mg/kg p.o of the extract, and 100 mg/kg silymarin an hour prior to 50 mg/kg CsA, respectively, for 5 days. Physical, biochemical, and histological parameters estimated by reported methods serves as scientific evidence in the study. Results: C. afer significantly restored catalase (P < 0.005), superoxide dismutase (P < 0.005), malondialdehyde (P < 0.005), and glutathione (P < 0.05) levels dose dependently. In all cases as mentioned previously, C. afer at all dose level promoted significant (P < 0.05) restoration of serum liver parameters such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, conjugated bilirubin, total bilirubin, total protein, and albumin. The biochemical observations were supplemented with histopathological reports, which showed attenuation of hepatocellular necrosis. Conclusion: Aqueous leaf extract of C. afer dose dependently prevented the significant changes on the physical, biochemical, and histological changes induced by CsA in the liver. Therefore, aqueous leaf extract of C. afer can be employed as an economic agent to treat liver diseases related to CsA administration and possibly other hepatotoxic drugs.

Keywords


Costus afer, Hepatocellular Necrosis, Liver Enzyme, Oxidative Stress, Silymarin.

References





DOI: https://doi.org/10.22506/ti%2F2015%2Fv22%2Fi3%2F137629