Toxicology International (Formerly Indian Journal of Toxicology)

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Macrophages and Lipid Peroxides Concentration of the Tissues:A Bio-Indicator of Ecological Hazard


Affiliations
1 Amity Institute of Biotechnology, Amity University, Noida - 201313, Uttar Pradesh, India
2 Magadh Mahila College, Patna University, Patna - 800001, Bihar, India
     

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A comprehensive study on the reproductive system of air breathing catfishes was undertaken to evaluate the effect of environmental toxicants on the breeding capacity of the catfishes, Heteropneustes fossilis. Histopathological examination of ovary by standard error of mean along with oxidative stress estimation measuring lipid peroxides and protein peroxides was carried on the ovary and the vital organs of 125 fishes from a polluted pond near Hindon river in Ghaziabad, Uttar Pradesh, India. Depending on the physico-chemical parameter of the polluted pond near Hindon river, it was found unhealthy and designated as Type E. Fresh fishes were taken as control from Gazipur, fish farm. Comparative study of ovarian follicles of control and polluted pond fishes was conducted to estimate the histopathology of the reproductive organ. Polluted pond fishes ovaries showed degenerative changes such as prominent longitudinal zig-zag folds, with eroded surface forming convex region on the margin of developing ovaries. Phagocytic macrophages were also seen present on the surface epithelium of the ovary. It was probably due to stressful condition induced by xenobiotics in the pond. Lipid peroxidation in different tissues was found more consistent in their action as compared to the protein peroxidation due to free radical generation by xenobiotics present in the pond. Thus, excess of macrophages and high level of lipid peroxidation of the tissues can be taken as bio-indicator of ecological hazards in an aquatic ecosystem.

Keywords

Heteropneustes fossilis, Lipid Peroxides, Macrophages, Malathion, Oocytes, Polluted Pond, Protein Peroxides, Standard Error of Mean, Ultrastructural.
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  • Sasaki YF, Izumiyama F, Nishidate E, Ishibashi S, Tsuda S, Matsusaka N, et al. Detection of genotoxicity of polluted sea water using shellfish and the alkaline single-cell gel electrophoresis (SCE) assay: A preliminary study. Mutat Res. 1997; 393:133–9.

  • Singh S, Sahai S. Effect of malathion on the immature ovary of Rasbora daniconius (Ham.) a histopathological study. Int J Acad Ichthyol (Proc. VAISHi). 1985a; 6:11–5.

  • Singh S, Sahai S. Effect of Endosulfan to the Ovaries of Rasbora daniconius (Ham.) Teleostei. National Seminar on Effects of Water Polution; 1985b. p. 21–2.

  • Harilal RS, Sahai YN. Effect of carbon on the gonads of Heteropneustes fossilis (Bloch). Environment and Ecotoxicology. Publisher in Academy of Environmental Biology; India. 1987. p. 35–49, 277–81.

  • Dutta HM, Nath A, Adhikari S, Roy PK, Singh MK, Munshi JS. Cell division and differentiation science. 102nd Annual Meeting the Ohio Academy of Science; Youngstown, State University. 1993 Apr 4-7 Apr.

  • Mani K, Saxena PK. Effect of safe concentration of some pesticides in ovarian recrudescence in the fresh water murrel Channa punctatus. A quantitative study. Ecotoxicol Environ Saf. 1985; 9:241–9.

  • Saxena PK, Garg M. Effect of insecticidal pollution on ovarian recrudescence in the fresh water teleost Channa punctatus (Bl). Indian J Exp Biol. 1978; 16:689–91.

  • Pandey AK, Shukla L. Ovarian recrudescence in freshwater teleost Sarotherodon mossambicus on DOT treatment and pattern of recoupment. J Environ Biol. 1985; 3:195–204.

  • Ojha S, Norton SP, Shrivastava N, Jain S. Toxic effect of malathion on the reproductive system of albino rats. Environ Ecol. 1992; 10:833–6.

  • Chowdhary DN, Sahay GR, Singh JN. Antifertility and canniballistic properties of some mycotoxins in albino rats. J Food Sci Technol. 1994; 31:497–9.

  • Nath A, Kedia OK. Toxic effect of malathion on the spermatozoa of Heteropneustes fossillis. Trans Zoolog Soc India. 1997; 1:99–102.

  • Singh S, Malik D, Thakur J, Kaur A, Singh RK, Nijhawan S. A comparative analysis of the physico-chemical properties and heavy metal pollution in three major rivers across India. Int J Sci Res. 2014; 3:1936–41.

  • Sehgal M, Garg A, Suresh R, Dagar P. Heavy metal contamination in the Delhi segment of Yamuna basin, centre for environmental studies. New Delhi: The Energy and Resources Institute; 2012.

  • Sen I, Shandil A, Shrivastava VS. Study for determination of heavy metals in fish species of the river Yamuna (Delhi) by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICPOES). Adv Appl Sci Res. 2011; 2:161–6.

  • Sprague JB. The ABC’s of pollutant bioassay using fish. In biological methods for the assessment of water quality. Am Soc Test Mater Technol Publ. 1973; 528:6–36.

  • Ismail M, Ali R, Ali T, Waheed U, Khan QM. Evaluation of acute toxicity of profenofos and its effect on behavior pattern of fingerling common carp (Cyprinus carpio L.) 1758. Bull Envi Contom Toxicol. 2009; 82:569–73.

  • APHA, AWWA, WPCF. Standard Methods for the Examination of Water and Waste Water. 16th ed. Washington, D.C: American Public Health Association, American Water Works Association, Water Pollution Control Federation; 1985.

  • Gopal B, Asthana V. Aquatic sciences in India. 1991. p. 12. [Adsorbs/3-78-79].

  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351–8.

  • Gay C, Collins J, Gebicki JM. Hydroperoxide assay with the ferric xylenol orange complex. Anal Biochem. 1999; 273:149–55.

  • Dutta HM, Nath A, Adhikari S, Roy PK, Singh MK, Munshi JS. Sublethal malathion induced changes in the ovary of an air breathing fish Heteropneustes fossilis: A histological study. Hydrobiologia. 1994; 294:2213–8.

  • Darnell J, Lodish H, Baltimore D. Molecular cell biology. New York: Scientific American Books; 1986.

  • Pareda J, Corral E, Pozo J. Congress XI International. Congress on Electron Microscopy; Kyoto. 1986. p. 2989–90.

  • Bloom W, Fawcett DW. A Text Book of Histology. Philadelphia, London: W.B. Saunders Company; 1962. p. 80.

  • Nath A, Jamuar MP. Ultrastructural changes in the sertoli cells during spermatogenic cycle of hanstigine 4th international. Congress Cell Biology; Madrid, Spain. 1992.

  • Munshi JSD, Singh NK, Mishra N, Ojha J. Cytology of macrophages in normal and mercury treated air breathing fish C. batrachus (Bloch). J Fish Biol. 1990; 37:651–3.

  • Nath A, Chand GB. Malathion induced spermiophagy in the testes of Heteropneustes fossilis, SEM study. Indian J Freshw Biol. 1997; 9:158–61.

  • Azmi MW, Bongso TA. Ultrastructure of sertoli cells during prepuberty and puberty. Proceedings 11th International. Congress on Election Microscopy; Kyoto. 1986. p. 2953.

  • Murakami M, Nishida TR, Shiromol M. SEM observation of the human testes with special reference to epithelial spermiophagy. Proceedings 11th International Congress on Electron Microscopy; Kyoto. 1986. p. 2955–7.

  • Motta PM, Makabe S. Occurrence of germ cells in the surface of human developing ovary. Proceedings 11th International. Congress on Electron Microscopy; Kyoto. 1986. p. 2987–8.

  • Saha K, Das K, Sahu A. Antibody dependent haemolysin, compliment and opsanin in seara of a major carp Cirrhina mrigala and cat fish Clarias batrachus and Heteropneustes fossilis. Comp Immunol Microbiol Infect Dis. 1993; 16:323–30.

  • Srivastava RK, Yadav KK, Trivedi SP. Devicyprin induced gonadal impairment in a fresh water food fish, Channa punctatus (Bloch). J Environ Biol. 2008; 29:187–91.

  • Bagchi M, Stohs SJ. In vitro induction of reactive oxygen species by 2,3,7,8 – TetraChlorobenzo-p-dioxin, endrin and lindane in rat peritoneal macrophages, hepatic mitrochondria and microsomes. Free Radic Biol Med. 1993; 14:11–8.

  • Thomas P, Wofford HW. Effects of cadmium and Aroclor 1254 on lipid peroxidation, glutathione peroxidase activity and selected antioxidant in Atlantic croaker tissues. Aquat Toxicol. 1993; 27:159–78.

  • Kale RK, Sitaswad SL. Lipid peroxidation and membrane damage. Radiat Phys Chem. 1990; 36:361–8, 31.

  • Giri AN, Srivastava DK, Trivedi SP. Insecticide basathrin induced histoanatomical insult of ovarian tissue of Indian catfish, Heteropneustes fossilis. Biol Mem. 2000; 26:20–4.


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  • Macrophages and Lipid Peroxides Concentration of the Tissues:A Bio-Indicator of Ecological Hazard

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Authors

Kumud Bala
Amity Institute of Biotechnology, Amity University, Noida - 201313, Uttar Pradesh, India
Manisha Lall
Magadh Mahila College, Patna University, Patna - 800001, Bihar, India
Abha Kumari
Amity Institute of Biotechnology, Amity University, Noida - 201313, Uttar Pradesh, India
Nidhi Srivastava
Amity Institute of Biotechnology, Amity University, Noida - 201313, Uttar Pradesh, India

Abstract


A comprehensive study on the reproductive system of air breathing catfishes was undertaken to evaluate the effect of environmental toxicants on the breeding capacity of the catfishes, Heteropneustes fossilis. Histopathological examination of ovary by standard error of mean along with oxidative stress estimation measuring lipid peroxides and protein peroxides was carried on the ovary and the vital organs of 125 fishes from a polluted pond near Hindon river in Ghaziabad, Uttar Pradesh, India. Depending on the physico-chemical parameter of the polluted pond near Hindon river, it was found unhealthy and designated as Type E. Fresh fishes were taken as control from Gazipur, fish farm. Comparative study of ovarian follicles of control and polluted pond fishes was conducted to estimate the histopathology of the reproductive organ. Polluted pond fishes ovaries showed degenerative changes such as prominent longitudinal zig-zag folds, with eroded surface forming convex region on the margin of developing ovaries. Phagocytic macrophages were also seen present on the surface epithelium of the ovary. It was probably due to stressful condition induced by xenobiotics in the pond. Lipid peroxidation in different tissues was found more consistent in their action as compared to the protein peroxidation due to free radical generation by xenobiotics present in the pond. Thus, excess of macrophages and high level of lipid peroxidation of the tissues can be taken as bio-indicator of ecological hazards in an aquatic ecosystem.

Keywords


Heteropneustes fossilis, Lipid Peroxides, Macrophages, Malathion, Oocytes, Polluted Pond, Protein Peroxides, Standard Error of Mean, Ultrastructural.

References





DOI: https://doi.org/10.22506/ti%2F2015%2Fv22%2Fi3%2F137635