Toxicology International (Formerly Indian Journal of Toxicology)

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2, 5-Hexanedione-Induced Oxidative Damage and DNA Fragmentation:Ameliorative Role of Rutin Ex Vivo


Affiliations
1 Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
2 Department of Food Technology, Federal Institute of Industrial Research, Oshodi, Lagos State, Nigeria
3 Department of Pharmacognosy and Ethnopharmacy, Usmanu Danfodiyo University, Sokoto, Nigeria
     

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The aim of this research was to evaluate the ameliorative effects of rutin on 2, 5-hexanedione-induced oxidative damage and DNA fragmentation in the viscera of Wistar rat ex vivo. Blood and tissues homogenates from normal Wistar rats weighing 150-200 g were used for the present investigation. The treatment with 2,5-hexanedione and/or rutin was for four (4), eight (8) and sixteen (16) hours incubation period at room temperature, and used non-specifically at the final concentrations of 0.0, 10.0 μM, 100.0 μM, 1.0 mM and 10.0 mM respectively. The experiments were done in four sequential stages involving the determination of lipid peroxidation and DNA fragmentation by standard procedures. 2,5-hexanedione dose/time-dependently caused a significant (P < 0.05) oxidative damage in the blood and tissues via lipid peroxidation and DNA fragmentation relative to control. Rutin administration was able to significantly (P < 0.05) suppress the 2,5-hexanedione-induced oxidative and DNA damage. However, the effective toxicity of 2,5-hexanedione and ameliorative effects of rutin were most versus least pronounced in liver (at 10 μM) and pancreas (at 1 mM) homogenates at 8hrs incubation time, respectively. 2,5-hexanedione in the viscera of Wistar rat induces oxidative and DNA damage characterised by higher level of malondialdehyde and DNA fragmentation. However, rutin administration was able to ameliorate these effects ex vivo.

Keywords

2, 5-Hexanedione, Ameliorative, DNA Fragmentation, Lipid Peroxidation, Rutin.
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  • Chen R, Liu S, Piao F, Wang Z, Qi Y, Li S, Zhang D, Shen J. Stem cells from rat bone marrow via mitochondria- dependent caspase-3 pathway. Ind Health. 2015; 53:222–35.

  • Adedara IA, Abolaji AO, Odion BE, Okwudi IJ, Omoloja AA, Farombi EO. Impairment of Hepatic and Renal Functions by 2 , 5-Hexanedione Is Accompanied by Oxidative Stress in Rats. J Toxicol. 2014; 2014:1–9

  • Boekelheide K, Fleming SL, Allio T, Embree-Ku ME, Hall SJ, Johnson KJ, KwonEJ, Patel SR, Rasoulpour RJ, Schoenfeld HA, Thompson S. 2,5-hexanedione-induced testicular injury. Annu Rev Pharmacol Toxicol. 2003; 43:125–47.

  • Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT - Food Sci Technol. 2008; 41:1060–6.

  • Marcarini JC, Tsuboy MSF, Luiz RC, Ribeiro LR, Hoffmann-Campo CB, Mantovani MS. Investigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells. Exp Toxicol Pathol. 2011; 63(5):459–65.

  • Kim G-N, Kwon Y-I, Jang H-D. Protective mechanism of quercetin and rutin on 2,2’-azobis(2-amidinopropane)dihydrochloride or Cu2+-induced oxidative stress in HepG2 cells. Toxicol In Vitro. 2011; 25(1):138–44.

  • Nafees S, Rashid S, Ali N, Hasan SK, Sultana S. Rutin ameliorates cyclophosphamide induced oxidative stress and inflammation in Wistar rats: Role of NFκB/MAPK pathway. Chem Biol Interact. 2015; 231:98–107.

  • Kamalakkannan N, Prince PSM. Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats. Basic Clin Pharmacol Toxicol. 2006; 98(1):97–103.

  • Khan MM, Ahmad A, Ishrat T, Khuwaja G, Srivastawa P, Khan MB, Raza SS, Javed H, Vaibhav K, Khan A, Islam F. Rutin protects the neural damage induced by transient focal ischemia in rats. Brain Res. 2009; 1292:123–35.

  • Sarkozi K, Papp A, Mate Z, Horvath E, Paulik E, Szabo A. Rutin, a flavonoid phytochemical, ameliorates certain behavioral and electrophysiological alterations and general toxicity of oral arsenic in rats. Acta Biol Hung. 2015; 66(1):14–26.

  • Abolaji AO, Kamdem JP, Lugokenski TH, Nascimento TK, Waczuk EP, Farombi EO, Loreto EL, Rocha JBT. Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster. Free Radic Biol Med. 2014; 71:99–108.

  • Ohkawa H, Ohishi N. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351–8.

  • Sellins KS, Cohen JJ. Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes. J Immunol. 1987; 139:3199–206.

  • US Environmental protection agency. Toxicological review of n-Hexane no. 110–54–3; 2005.

  • Perbellini L, Mozzo P, Olivato D, Brugnone F. Dynamic’ biological exposure indexes for n-hexane and 2,5-hexanedione, suggested by a physiologically based pharmacokinetic model. Am Ind Hyg Assoc J. 1990; 51(7):356–62.

  • Mayan O, Teixeira JP, Alves S, Azevedo C. Urinary 2,5 hexanedione as a biomarker of n-hexane exposure. Biomarkers. 2002; 7(4):299–305.

  • Song F, Zhang Q, Kou R, Zou C, Gao Y, Xie K. 2,5-Hexanedione altered the degradation of low-molecular-weight neurofilament in rat nerve tissues. Food Chem Toxicol. 2012; 50(12): 4277–84.

  • Cardona A, Marhuenda D, Prieto MJ, Martí J, Periago JF, Sánchez JM. Behaviour of urinary 2,5-hexanedione in occupational co-exposure to n-hexane and acetone. Int Arch Occup Environ Health. 1996; 68(2):88–93.

  • Kohen R, Nyska A. Oxidation of biological systems: Oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. 2002; 30(6): 620–50.

  • Marnett LJ. Lipid peroxidation - DNA damage by malondialdehyde. Mutat Res Mol Mech Mutagen. 1999; 424(1–2):83–95.

  • Ladefoged O, Perbellini L. Acetone-induced changes in the toxicokinetics of 2,5-hexanedione in rabbits. Scand J Work Environ Heal. 1986; 12(6):627–9.

  • Myall OT, Allen SL, McLean WG. The effect of 2,5-hexanedione on the induction of ornithine decarboxylase in the dorsal ischolar_main ganglion of the rat. Neurosci Lett. 1990; 114(3):305–8.

  • De Caprio AP, Olajos EJ, Weber P. Covalent binding of a neurotoxic n-hexane metabolite: Conversion of primary amines to substituted pyrrole adducts by 2,5-hexanedione. Toxicol Appl Pharmacol. 1982; 65(3):440–50.

  • Carney R, Dardis C, Cullen WK, Felipo V, Anwyl R, Rowan MJ. Early spatial memory deficit induced by 2,5-hexanedione in the rat. Toxicol Lett. 2002; 128(1–3):107–15.

  • Guo R, Wei P, Liu W. Combined antioxidant effects of rutin and vitamin C in Triton X-100 micelles. J Pharm Biomed Anal. 2007; 43(4):1580–6.


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  • 2, 5-Hexanedione-Induced Oxidative Damage and DNA Fragmentation:Ameliorative Role of Rutin Ex Vivo

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Authors

Aliyu Muhammad
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Ochuko L. Erukainure
Department of Food Technology, Federal Institute of Industrial Research, Oshodi, Lagos State, Nigeria
Ibrahim Malami
Department of Pharmacognosy and Ethnopharmacy, Usmanu Danfodiyo University, Sokoto, Nigeria
Umoru Murtala
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Fadila I. Mora
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Aminu Suleiman
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Rahfat S. Abubakar
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
James A. Kehinde
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Zaynab M. K. Muhammed
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Ephraim E. Alahirah
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria

Abstract


The aim of this research was to evaluate the ameliorative effects of rutin on 2, 5-hexanedione-induced oxidative damage and DNA fragmentation in the viscera of Wistar rat ex vivo. Blood and tissues homogenates from normal Wistar rats weighing 150-200 g were used for the present investigation. The treatment with 2,5-hexanedione and/or rutin was for four (4), eight (8) and sixteen (16) hours incubation period at room temperature, and used non-specifically at the final concentrations of 0.0, 10.0 μM, 100.0 μM, 1.0 mM and 10.0 mM respectively. The experiments were done in four sequential stages involving the determination of lipid peroxidation and DNA fragmentation by standard procedures. 2,5-hexanedione dose/time-dependently caused a significant (P < 0.05) oxidative damage in the blood and tissues via lipid peroxidation and DNA fragmentation relative to control. Rutin administration was able to significantly (P < 0.05) suppress the 2,5-hexanedione-induced oxidative and DNA damage. However, the effective toxicity of 2,5-hexanedione and ameliorative effects of rutin were most versus least pronounced in liver (at 10 μM) and pancreas (at 1 mM) homogenates at 8hrs incubation time, respectively. 2,5-hexanedione in the viscera of Wistar rat induces oxidative and DNA damage characterised by higher level of malondialdehyde and DNA fragmentation. However, rutin administration was able to ameliorate these effects ex vivo.

Keywords


2, 5-Hexanedione, Ameliorative, DNA Fragmentation, Lipid Peroxidation, Rutin.

References





DOI: https://doi.org/10.22506/ti%2F2015%2Fv22%2Fi3%2F137638