Open Access
Subscription Access
Open Access
Subscription Access
In vivo Genotoxicity Evaluation of Carbaryl Pesticides Using Polytene Chromosomes of Anopheles culicifacies (Diptera:Culicidae)
Subscribe/Renew Journal
Carbamate pesticides are in tremendous use in modern agricultural practices due to their relatively low persistence in environment but their impacts on genetic imprints of living organism are well documented by various investigations, therefore genotoxicity evaluation of such pesticides has become crucial before their extensive commercial use. The present research work deals with genotoxicity assessment of a carbamate pesticide viz: carbaryl by using genetic material of Anopheles culicifacies. To achieve the target, mosquito larvae were exposed to LC20 of selected pesticide for duration 24 hours and subsequently larval salivary polytene chromosomes were studied to observe detrimental consequences of carbaryl. The concerned carbamate pesticide was observed to induce different types of structural aberrations such as inversions, translocations, deletions, ectopic pairings, asynapses, chromosomal fusions and breaks. After statistical analysis of obtained data, values were calculated 31.35±9.07 in carbaryl exposed stocks as compared 19.36±4.63 in control groups. Subsequently, statically examination of procured data, from executed parameter, demonstrated significant outcomes, when scrutinized through student't' test (p<0.05).
Keywords
Polytene Chromosomes, Carbaryl and Anopheles culicifacies.
User
Subscription
Login to verify subscription
Font Size
Information
- Ghosh P, Bhattacharya S and Bhattacharya S. Impairement of regulation of gonadal function in Channa panctatus by metacid- 50 and carbaryl under laboratory and field conditions. Biomed Environ Sci 1990; 3 (1): 106-12.
- Schreinemachers D M. Birth defects higher in babies born to families living near farming areas using pesticides. Environ Health Perspect 2003; 111(9):1259-64.
- Richard A, Sridhar C, Vaughan GM. Breast cancer linked to home pesticide chlordane. Breast Cancer Res Treat 2005; 90:55-64.
- Sharma GP, Sobti RC, Chaudhry A, Ahluwalia KK . Chromosomal aberrations and dominant lethal in Culex fatigans due to mercuric chloride. Cytobios 1989; 59: 131-35.
- Chaudhry A, Anand PK, Geeta, Singh S, Lovleen. Ectopic pairing of the intercalary heterochromatin in the organophosphate pesticide treated mosquito chromosome (Culicidae: Diptera). Cytologia 2006; 71(4): 431-37.
- Chaudhry A, Lovleen. Evaluation of mutagenic potential of carbaryl by dominant lethal test on Culex quinquefasciatus. J Cytol Genet. 2008 ;9: 37-44.
- Chaudhry A, Lovleen. Chromosomal aberrations and dominant lethal test based genotoxicity assessment of dicofol by using mosquito genome. J Cytol Genet 2010; 11: 11-20.
- Lovleen . In vivo mutagenicity assessment of ethion pesticide using polytene chromosomes of Anopheles culicifacies (Diptera: Culicidae). AJPT 2015; 3(9): 1-7.
- Tripathi PK, Singh A. Carbaryl induced alterations in the reproduction and metabolism of freshwater snail Lymnaea acuminata. Pestic Biochem Phys 2004; 79: 1-9.
- Delescluse C, Ledirac N, Li R, Piechocki MP, Hines RN, Gidrol X, Rahmani R . Induction of cytochrome P4501A1 gene expression, oxidative stress and genotoxicity by carbaryl and thiabendazole in transfected human HepG2 and lymphoblastoid cells. Biochem. Pharmacol 2000; 61: 39907.
- Singh OP, Raghavendra K, Nanda N, Mittal PK, Subbarao SK. Pyrethroid resistance in Anopheles culicifacies in Surat district, Gujarat, west India. Cur Sci. 2002; 82(5): 547-50.
- Wattal BL, Kalra N L. Regionwise pictorial keys to the female Indian Anopheles. Bull Nat Soc Ind Mal Mosq Born Dis, 1967; 9: 133-38.
- Singh KRP, Brooks GD, Ansari MA. Mass rearing of mosquitoes. J Com Dis 1974; 6: 121-26.
- Singh KRP, Patterson RS, Labrecque GC, Razdan RK. Mass rearing of Culex pipiens fatigans Weid . J Com Dis 1975; 7: 31-53.
- Finney DJ. Probit analysis. Cambridge University Press, Cambridge, 1971; 3: 237-45.
- French WL, Baker RH, Kitzmiller JB. Preparation of mosquito chromosomes. Mosq News 1962; 22: 377-83.
- Michailova, P., Todorova, K. and White K. The effect of lead on the salivary gland chromosomes of Glyptotendipes salinus (Chironomidae, Diptera). Biologia, 2002; 57(3): 35967.
- Barr HJ, Ellison JR. Ectopic pairing of chromosome regions containing chemically similar DNA. Chromosoma 2004; 39: 53-61.
- Michailova PV. Rearrangements in Chironomidae (Diptera) genomes induced by various environmental stress factor. Becmhunk. Botuc 2010; 14: 43-53.
- Michailova P, Petrova N., Ilkova J, Bovero S, Brunetti S, White K, Sella, G. Genotoxic effect of copper on salivary gland chromosomes of Chironomus riparius Meigen 1804 (Diptera: Chironomidae). Environ Pollut 2006; 144(2): 647-54.
- Ahmed FE, Hart RW, Lewis NJ. Pesticide induced DNA damage and its repair in cultured human cells. Mutat Res, 1977; 42:161-74.
- Grisolia CK. A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin C and various pesticides. Mutat Res. 2002 ; 518(2): 145-50.
- Grover IS, Ladhar SS, Randhawa SK.. Carbaryl-A selective genotoxicant. Environ Pollut 1989; 58: 313-23.
- Wyrobek AJ, Watchmaker G, Gordon L, Wong K, Moore D, Whorton D. Sperm shape abnormalities in carbaryl-exposed employees. Environ Health Perspect 1981; 40: 255.
- Xia Y, Cheng S, Bian Q, Xu L, Collin MD, Chang HC, Song L . Genotoxic effects on spermatozoa of carbaryl–exposed workers. Toxicol Sci 2004 ; 85(1): 615-23.
- Meeker J D, Singh N P, Ryan L, Duty S M, Barr D B, Harick R F, Bennett D H, Hauser R . Human semen quality and sperm DNA damage in relation to urinary metabolites of pyrethroid insecticides. Hum Reprod 2004;19: 2573-80.
Abstract Views: 602
PDF Views: 0