Toxicology International (Formerly Indian Journal of Toxicology)

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Kolaviron and Garcinia kola Attenuate Homocysteine-Induced Arteriosclerosis and Cardiotoxicity in Wistar Rats


Affiliations
1 Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Nigeria
2 Department of Veterinary Medicine, University of Ibadan, Nigeria
3 Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria
     

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Sixty male Wistar rats were divided into six groups of 10 rats each. Group I was the control. Group II received homocysteine (Hcy) (200 mg/kg body weight) alone daily for 14 days. Rats in groups III and IV received Kolaviron orally at 100 and 200 mg/kg respectively, in addition to Hcy. Rats in groups V and VI received Garcinia kola (GK) orally (100 and 200 mg/ kg respectively) in addition to Hcy (200 mg/kg) for a period of 14 days. The results showed that Hcy caused a reduction in heart rate, shortened QT and QTc intervals together with low voltage QRS which were reversed by Kolaviron (100&amp;200 mg/kg body weight) and Garcinia kola (100&200 mg/kg body weight). Hcy alone also caused a significant (p<0.05) increase in MDA, H2O2 generation, Xanthine oxidase, Myeloperoxidase, Lactate dehydrogenase (LDH), Creatinine kinase cardiac specific (CK-MB) and nitrite with a significant (p<0.05) reduction in non-enzymic and enzymic antioxidants such as Reduced glutathione (GSH), Glutathione peroxidase (GPx), Glutathione-s-transferase (GST), Superoxide dismutase (SOD) and Catalase (CAT) coupled with a significantly high serum values of total cholesterol, triglycerides and low density lipoproteins (LDL) and a significant reduction in (p<0.05) high density lipoprotein (HDL) cholesterol. Overall, co-administration of Hcy with Garcinia kola at 200 mg/kg body weight showed a better cardioprotective and antiatherosclerotic effect on (Hcy)-induced cardiotoxicity and atherosclerosis than Kolaviron in this study.

Keywords

Homocysteine (HCY), Garcinia kola, Kolaviron, Anti-Atherosclerosis, ECG Changes.
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  • Cao R, Bai Y, Xu R, et al. Homocysteine is associated with plasma high-sensitivity cardiac troponin T levels in a communitydwelling population. Clin Interv Aging. 2014; 9:7984.

  • Parnetti L, Caso V, Santucci A, et al. Hyperhomocysteinemia, a cardiac metabolic disease: role of nitric oxide and the p22phox subunit of NADPH oxidase. Neurol Sci. 2004; 25:13-7.

  • Wang X, Cui L, Joseph J, et al. Homocysteine induces cardiomyocyte dysfunction and apoptosis through p38 MAPK-mediated increase in oxidant stress. J Mol Cell Cardiol. 2012 52:753-760.

  • Levrand S, Pacher P, Pesse B, et al. Homocysteine induces cell death in H9C2 cardiomyocytes through the generation of peroxynitrite. Biochem Biophys Res Commun. 2007; 359:445-450.

  • Kolling J, Scherer EB, da Cunha AA, et al. Homocysteine induces oxidative-nitrative stress in heart of rats: prevention by folic acid. Cardiovasc Toxicol. 2011; 11:67-73.

  • Baszczuk A, Kopczyński Z, Thielemann A. Endothelial dysfunction in patients with primary hypertension and hyperhomocysteinemia. Postepy Hig Med Dosw. 2014 68:91-100.

  • Weiss N. Mechanisms of increased vascular oxidant stress in hyperhomocysteinemia and its impact on endothelial function. Curr Drug Metab. 2005; 6:27-36.

  • Adedara IA, Lawal TA, Adesina AA, et al. Sperm functional parameters and erythrocytes oxidant-antioxidant imbalance during municipal landfill leachate treatment withdrawal in rats. Environ Toxicol Pharmacol. 2014; 37:460467.

  • Iwu MM, Igboko OA, Onwuchekwa U, et al. Evaluation of the anti-hepatotoxicity of the biflavonoids of Garcinia kola seeds. Journal of Ethnopharmacology. 1987; 21:127-142.

  • Shinha KA. Colorimetric assay of Catalase. Anal. Biochem. 1972; 47: 389-394.

  • Misra HP, Fridovich I. The role of superoxide anoin in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 1972; 217: 3170-3175.

  • Habig WH, Pabst MJ, Jacoby WB. Glutathione-S-transferase Activity: The Enzymic step in Mercapturic Acid formation. J. Biol. Chem. 1974; 249: 130-139.

  • Lowry OH. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951; 193: 265- 275.

  • Jollow DJ, Mitchell JR, Zampaglione N, et al. Bromobenzeneinduced liver necrosis; protective role of GSH & evidence for 3, 4 bromobenzene oxide as the hepatotoxic metabolite. JB lippinocott. 1994; 108-144.

  • Wolff SP. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Method Enzymol. 1994; 233: 182-189.

  • Farombi EO, Tahnteng JG, Agboola AO, et al. Chemoprevention of 2 acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron a-Garcina kola seed extract. Food Chem. Toxicol. 200; 38: 353-541.

  • Buetler, E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963; 61: 882-888.

  • Akaike T, Ando M, Oda T, et al. Dependence on O2- generation by xanthine oxidase of pathogenesis of influenza virus infection in mice. J Clin Invest. 1990; 85:739-745.

  • Olaleye SB, Adaramoye OA, Erigbali PP, et al. Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol-exposed rats. World J Gastroenterol. 2007; 13:5121-126.

  • Xia Y, Zweier JL. Measurement of myeloperoxidase in leukocytecontaining tissues. Anal Biochem 1997; 245: 93-96.

  • Wang X, Cui L, Joseph J, et al. Homocysteine induces cardiomyocyte dysfunction and apoptosis through p38 MAPK-mediated increase in oxidant stress. J Mol Cell Cardiol. 2012; 52:753-760.

  • Garg DP, Bansal AK, Malhotra A, et al. (2009). Methomyl induced hematological and biochemical alterations-protection by vitamin E. Pestic Biochem Phys. 2009; 93:127-132.

  • Anatoliotakis N, Deftereos S, Bouras G, et al. Myeloperoxidase: expressing inflammation and oxidative stress in cardiovascular disease. Curr Top Med Chem. 2013; 13:115138.

  • Martin M. Small Animal ECGs an introductory guide. Second edition. Blackwell Publishing, Oxford, United Kingdom. 2007.

  • Vennila L, Pugalendi KV. Protective effect of sesamol against myocardial infarction caused by isoproterenol in Wistar rats. Redox Rep. 2010; 15:36-42.


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  • Kolaviron and Garcinia kola Attenuate Homocysteine-Induced Arteriosclerosis and Cardiotoxicity in Wistar Rats

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Authors

A. A. Oyagbemi
Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Nigeria
T. O. Omobowale
Department of Veterinary Medicine, University of Ibadan, Nigeria
E. O. Farombi
Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria

Abstract


Sixty male Wistar rats were divided into six groups of 10 rats each. Group I was the control. Group II received homocysteine (Hcy) (200 mg/kg body weight) alone daily for 14 days. Rats in groups III and IV received Kolaviron orally at 100 and 200 mg/kg respectively, in addition to Hcy. Rats in groups V and VI received Garcinia kola (GK) orally (100 and 200 mg/ kg respectively) in addition to Hcy (200 mg/kg) for a period of 14 days. The results showed that Hcy caused a reduction in heart rate, shortened QT and QTc intervals together with low voltage QRS which were reversed by Kolaviron (100&amp;200 mg/kg body weight) and Garcinia kola (100&200 mg/kg body weight). Hcy alone also caused a significant (p<0.05) increase in MDA, H2O2 generation, Xanthine oxidase, Myeloperoxidase, Lactate dehydrogenase (LDH), Creatinine kinase cardiac specific (CK-MB) and nitrite with a significant (p<0.05) reduction in non-enzymic and enzymic antioxidants such as Reduced glutathione (GSH), Glutathione peroxidase (GPx), Glutathione-s-transferase (GST), Superoxide dismutase (SOD) and Catalase (CAT) coupled with a significantly high serum values of total cholesterol, triglycerides and low density lipoproteins (LDL) and a significant reduction in (p<0.05) high density lipoprotein (HDL) cholesterol. Overall, co-administration of Hcy with Garcinia kola at 200 mg/kg body weight showed a better cardioprotective and antiatherosclerotic effect on (Hcy)-induced cardiotoxicity and atherosclerosis than Kolaviron in this study.

Keywords


Homocysteine (HCY), Garcinia kola, Kolaviron, Anti-Atherosclerosis, ECG Changes.

References





DOI: https://doi.org/10.22506/ti%2F2016%2Fv23%2Fi3%2F146718