Toxicology International (Formerly Indian Journal of Toxicology)

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Effect of Cadmium on Glucose, Lipid Profile and Oxidative Stress in Streptozotocin-Induced Diabetic and Non-Diabetic Rats


Affiliations
1 Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran, Islamic Republic of
2 Department of Immunogenetics, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran, Islamic Republic of
3 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran, Islamic Republic of
     

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Cadmium (Cd), highly toxic heavy metal, has been considered as a possible risk factor for diabetes and its complications. However, the underlying mechanisms of Cd-induced diabetes are not clear. The present study was designed to evaluate the effects of Cd on the oxidative system in the diabetic and non-diabetic rats. Rats were divided into the following groups of 8 animals each: control (C), diabetic (D), Cd-exposed (1 mg/kg/bw) and diabetic-exposed to Cd (1 mg/kg/bw) (D + Cd- exposed) groups. Diabetes was induced by streptozotocin (intraperitoneally (i.p.)) at a single dose of 60 mg/kg. Cd (i.p.) was administered 3 days after streptozotocin (STZ) administration to the end of the study. After 4-weeks, blood was drawn to determine the changes of glucose, serum superoxide dismutase (SOD), catalase (CAT), glutathione-S-transfrase (GST) and glutathione peroxidase (GPx) activities as well as the levels of reduced glutathione (GSH), malondialdehyde (MDA) and lipid profile. The results indicated that Cd exposure aggravated increased blood glucose, MDA, triglycerides, total cholesterol, LDL-C as well as decreased GSH levels and the activities of antioxidant enzymes in diabetic rats (p<0.001). These results suggest that Cd exposure deteriorates diabetic effect and its complications in STZ-diabetic model by induction of oxidative stress.

Keywords

Cadmium, Diabetes, Lipid Profile, Oxidative Stress, Rats.
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  • Effect of Cadmium on Glucose, Lipid Profile and Oxidative Stress in Streptozotocin-Induced Diabetic and Non-Diabetic Rats

Abstract Views: 772  |  PDF Views: 3

Authors

Saeed Samarghandian
Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran, Islamic Republic of
Abasalt Borji
Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran, Islamic Republic of
Tahereh Farkhondeh
Department of Immunogenetics, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran, Islamic Republic of
Asadi-Samani Majid
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran, Islamic Republic of

Abstract


Cadmium (Cd), highly toxic heavy metal, has been considered as a possible risk factor for diabetes and its complications. However, the underlying mechanisms of Cd-induced diabetes are not clear. The present study was designed to evaluate the effects of Cd on the oxidative system in the diabetic and non-diabetic rats. Rats were divided into the following groups of 8 animals each: control (C), diabetic (D), Cd-exposed (1 mg/kg/bw) and diabetic-exposed to Cd (1 mg/kg/bw) (D + Cd- exposed) groups. Diabetes was induced by streptozotocin (intraperitoneally (i.p.)) at a single dose of 60 mg/kg. Cd (i.p.) was administered 3 days after streptozotocin (STZ) administration to the end of the study. After 4-weeks, blood was drawn to determine the changes of glucose, serum superoxide dismutase (SOD), catalase (CAT), glutathione-S-transfrase (GST) and glutathione peroxidase (GPx) activities as well as the levels of reduced glutathione (GSH), malondialdehyde (MDA) and lipid profile. The results indicated that Cd exposure aggravated increased blood glucose, MDA, triglycerides, total cholesterol, LDL-C as well as decreased GSH levels and the activities of antioxidant enzymes in diabetic rats (p<0.001). These results suggest that Cd exposure deteriorates diabetic effect and its complications in STZ-diabetic model by induction of oxidative stress.

Keywords


Cadmium, Diabetes, Lipid Profile, Oxidative Stress, Rats.

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DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi1%2F149027