Toxicology International (Formerly Indian Journal of Toxicology)

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Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster


Affiliations
1 Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
2 Laboratorio de Genetica y Biologia Molecular, Departamento de Biologia, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
3 Instituto de Investigaciones Clínicas “Dr. Américo Negrette”, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
     

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We determined the effect of long-term administration (12 days) of minocycline (Mino) on the oxidative stress induced by paraquat (PQ) in D. melanogaster. After intoxication with PQ (40 mM) for 36 hours, the intoxicated group was subdivided in three groups: PQ (no further treatment after PQ intoxication), PQ-Mino (treated with Mino [0.05 mM] for 12 days after intoxication) and PQ-Control (exposed to PQ and maintained in standard medium for 12 days after intoxication). Two additional groups, not treated with PQ, were added: Control and Mino. After 36 hours intoxication the concentrations of H2O2, the activities of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in the PQ group were increased, but the levels of nitrite and glutathione (GSH) were reduced with respect to the Control. In the group PQ also were observed an increase in the concentrations of MDA, protein carbonyl and the 8-hydroxydeoxyguanosine (8-OHdG) compared with Control. The post-treatment by 12 days with minocycline (PQ-Mino) mitigated oxidative damage induced by PQ as evidenced by the reduction in the concentration of the oxidative stress markers and by decrease damage to the lipid membrane (MDA), protein (protein carbonyl), DNA (8-OHdG) and in an increase of the extension of the life cycle of the flies with respect to PQ-Control.

Keywords

Drosophila melanogaster, Minocycline, Oxidative Stress, Paraquat.
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  • Blesa J, Phani S, Jackson-Lewis V. Przedborski S. Classic and new animal model of Parkinson´s disease. J Biomed Biotechnol. 2012;2012:845618.

  • Cochemé HM, Murphy M. Complex I is the major site of mitochondrial superoxide production by paraquat. J Biol Chem. 2008;283:1786-98.

  • Denicola A, Radic R. Peroxynitrite and drug-dependent toxicity. Toxicology. 2005;208:273–88.

  • Day BJ, Patel M, Calavetta L, Chang, Stamler JS. A mechanism of paraquat toxicity involving nitric oxide synthase. Proc Natl Acad Sci U.S.A. 1999;96:12760-65.

  • Kalyanaraman B. Teaching the basics of redox biology to medical and graduate students: Oxidants, antioxidants and disease mechanisms. Redox Biol. 2013;1:244-57.

  • Navarro JA, Hebner S, Yenisetti SC, Bayersdorfer F, Zhang L, Voigt A, et al. Analysis of dopaminergic neuronal dysfunction in genetic and toxin-induced models of Parkinson’s disease in Drosophila. J Neurochem. 2014;131:369-82.

  • Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2’-deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. Journal of environmental science and health. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009;27:120-39.

  • Plane JM, Shen Y, Pleasure DE, Deng W. Prospects for Minocycline Neuroprotection. Arch Neurol. 2010;67:1442– 48.

  • Selva P, Lakshmi S. Anti-Oxidant activity of minocycline An In vitro Study. Int J Innovat Drug Discov. 2013;3:51-4.

  • Huang CL, Lee YC, Yang YC, Kuo TY, Huang NK. Minocycline prevents paraquat-induced cell death through attenuating endoplasmic reticulum stress and mitochondrial dysfunction. Toxicol Lett. 2012;209:203-10

  • Bonilla E, Medina-Leendertz S, Villalobos V, Molero L, Bohorquez A. Paraquat - induced oxidative stress in Drosophila melanogaster: Effects of melatonin, glutathione, serotonin, minocycline, lipoic acid and ascorbic acid. Neurochem Res. 2006;31:1425-32.

  • Fernández-Vizarra E, Ferrín G, Pérez-Martos A, FernándezSilva AP, Zeviani M, Enríquez JA. Isolation of mitochondria for biogenetical studies: An update. Mitochondrion. 2010;10: 253-62.

  • Mora M, Bonilla E, Medina-Leendertz S, Bravo Y, Arcaya JL. Minocycline increases the activity of superoxide dismutase and reduces the concentration of nitric oxide, hydrogen peroxide and mitochondrial malondialdehyde in manganese treated Drosophila melanogaster. Neurochem Res. 2014;39:1270-78.

  • Hosamani R, Muralidhara. Acute exposure of Drosophila melanogaster to paraquat causes oxidative stress and mitochondrial dysfunction. Arch Insect Biochem Physiol. 2013;83:25-40.

  • Mora M, Medina-Leendertz S, Bonilla E, Terán RE, Paz MC, Arcaya JL. Minocycline, but not ascorbic acid, increases motor activity and extends life span of Drosophila melanogaster. Invest Clin. 2013;54:161-70.

  • Inamdar AA, Chaudhuri A, O’Donnell J. The protective effect of minocycline in a paraquat-induced Parkinson’s disease model in Drosophila is modified in altered genetic backgrounds. Parkinsons Dis. 2012;2012:938528.

  • Mehdi SH, Qamar A. Paraquat-induced ultrastructural changes and DNA damage in the nervous system is mediated via oxidative-stress-induced cytotoxicity in Drosophila melanogaster. Toxicol Sci. 2013;134:355-65.

  • Hosamani R, Muralidhara. Prophylactic treatment with Bacopa monnieri leaf powder mitigates Paraquat induced oxidative perturbations and lethality in Drosophila melanogaster. Indian J Biochem Bio. 2010;47:75-2.

  • Narayanasamy SK, Simpson DC, Martin I, Grotewiel M, Gronert S. Paraquat exposure and Sod2 knockdown have dissimilar impacts on the Drosophila melanogaster carbonylated protein proteome. Proteomics. 2014;14:2566-77.

  • Ajjuri RR, O’Donnell JM. Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response. J Vis Exp. 2013;82: e50892.

  • Djukic M, Jovanovic MC, Ninkovic M, Vasiljevic I, Jovanovic M. The role of nitric oxide in paraquat-induced oxidative stress in rat striatum. Ann Agric Environ Med. 2007;14:247-52.

  • Lin S, Zhang Y, Dodel R, Farlow MR, Paul SM, Du Y. Minocycline blocks nitric oxide-induced neurotoxicity by inhibition p38 MAP kinase in rat cerebellar granule neurons. Neurosci. Lett. 2001;315:61-64.


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  • Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster

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Authors

E. Bonilla
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
S. Medina-Leendertz
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
M. Mora
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
J. R. Vielma
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
L. Atencio-Bracho
Laboratorio de Genetica y Biologia Molecular, Departamento de Biologia, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of
Y. Bravo
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
M. Paz
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
A. Leal-Yepez
Laboratorio de Neurobiologia, Centro de Investigaciones Biomedicas, Instituto Venezolano de Investigaciones Científicas (IVIC), Estado Zulia, Venezuela, Bolivarian Republic of
J. L. Arcaya
Instituto de Investigaciones Clínicas “Dr. Américo Negrette”, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela, Bolivarian Republic of

Abstract


We determined the effect of long-term administration (12 days) of minocycline (Mino) on the oxidative stress induced by paraquat (PQ) in D. melanogaster. After intoxication with PQ (40 mM) for 36 hours, the intoxicated group was subdivided in three groups: PQ (no further treatment after PQ intoxication), PQ-Mino (treated with Mino [0.05 mM] for 12 days after intoxication) and PQ-Control (exposed to PQ and maintained in standard medium for 12 days after intoxication). Two additional groups, not treated with PQ, were added: Control and Mino. After 36 hours intoxication the concentrations of H2O2, the activities of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in the PQ group were increased, but the levels of nitrite and glutathione (GSH) were reduced with respect to the Control. In the group PQ also were observed an increase in the concentrations of MDA, protein carbonyl and the 8-hydroxydeoxyguanosine (8-OHdG) compared with Control. The post-treatment by 12 days with minocycline (PQ-Mino) mitigated oxidative damage induced by PQ as evidenced by the reduction in the concentration of the oxidative stress markers and by decrease damage to the lipid membrane (MDA), protein (protein carbonyl), DNA (8-OHdG) and in an increase of the extension of the life cycle of the flies with respect to PQ-Control.

Keywords


Drosophila melanogaster, Minocycline, Oxidative Stress, Paraquat.

References





DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi1%2F149037