![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_open_medium.gif)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltext_restricted_medium.gif)
Clastrogenic Instrict of Sodium Bispyribac and Pinoxaden Herbicides on Drosophila melanogaster
Subscribe/Renew Journal
The present research is attributed to evaluate the genotoxic potential of two herbicides namely Sodium Bispyribac and Pinoxaden, at sublethal level, LC20 using larval salivary gland polytene chromosomes of Drosophila melanogaster. Standardization of exposure concentration was achieved by exposing second instars larvae of Drosophila to serial dilution concentration of the selected herbicides, for continuum of 24 hours. Thereafter, on the basic of observed mortality in treated and control groups, exact value of LC20 was calculated by applying probit analysis and the corresponding values are 4.4 pl/ml for Sodium Bispyribac and 7.1pl/ml for Pinaxoden. Subsequently, exposure of standarized concentration was provided to second instar larvae, by mixing specified concentration in culture medium and, simultaneously negative control were also maintained. Third instar larvae were sacrificed for temporary squash preparation of polytene chromosomes and chromosomal complement were scrutinized for various types of structural chromosomal abnormities, in herbicide exposure group and natural population. Procured data indicated that the selected herbicides affected the structural integrity of polytene chromosomes, by inducing intrachromosomal, interchromosomal and telomeric fusions, paracentric inversions, inversion complexes, intrachromosomal and interchromosomal ectopic pairings due to induced homozygocity, chromatid breaks, chromosomal translocations and homologous chromosomal asynapses with respective value with respective number 45.66 ± 6.65 and 21 ± 3.60 in Sodium Bispyribac treated individuals and control, whereas in Pinoxaden treated and controls value 48.64 ± 8.6 and 25.32 ± 3.66 in control group. Subsequently, analysis of procured data, indicated that selected herbicides induced statistical significant genotoxicity in treated individuals in comparison to control groups (p<0.05).
Keywords
Genotoxicity, Bispyribac, Pinoxaden, Polytene, Drosophila.
User
Subscription
Login to verify subscription
Font Size
Information
- Ghosh P, Bhattacharya S. and Bhattacharya S. Impairement of regulation of gonadal function in Channa panctatus by metacid- 50 and carbaryl under laboratory and field conditions. Biomed Environ Sci 1990; 3 (Pt1): 106-2.
- Kumar L P, and Panneerselvam N. Toxic effects of pesticides: A Review on cytogenetic biomonitoring studies. Med Biol 2008 15(Pt2); 46-5.
- Chaturvedi M, Sharma C, Chaturvedi M. Effects of pesticides on human beings and farm animals: A case study. RJCES 2013; 1:14-9.
- Andersson H, Tago D, Triech N. Pesticides and health: A review of evidence on health effects valuation of risks, and benefit cost analysis. Adv Health Econ Health Serv Res 2014; 24: 203-5.
- Marwaha L. Genotoxicity assessment of an organophosphate insecticide, ethion using mosquito genome (Diptera: Culicidae). Toxicology International 2015; 22(Pt2):25-3.
- http://dnr.wi.Gov/lakes/plants/factsheets /BispyribacSodiumFactsheet.pdf.
- http://www.herbiguide. com.au/Descriptions/hg_Pinoxaden_100.htm.
- Sharma G P, Sobti R C, Chaudhry A, Ahluwalia K K. Genotoxicity of two heavy metal compounds lead acetate and mercuric chloride in Anopheles stephensi liston (Diptera: Culicidae). Cytologia 1988; 53:263-7.
- Chaudhry A, Lovleen. Evaluation of mutagenic potential of carbaryl by dominant lethal test on Culex quinquefasciatus. J Cytol Genet 2008; 9: 37-4.
- Chaudhry A, Lovleen. Chromosomal aberrations and dominant lethal test based genotoxicity assessment of dicofol by using mosquito genome. J Cytol Genet 2010; 11: 11-2.
- Lovleen. In vivo mutagenicity assessment of ethion pesticide using polytene chromosomes of Anopheles culicifacies (Diptera: Culicidae). AJPT 2015a; 3: 1-6.
- Lovleen. Mutagenicity analysis of ethion using Culex quinquefasciatus (Diptera: Culicidae). Journal of Chemical and Pharmaceutical Research 2015b; 7: 533-9.
- Lovleen. Genotoxicity evaluation of carbaryl using Culex quinquefasciatus (Diptera: Culicidae) RJPBCS 2015c; 6: 339-9.
- Lovleen. rDNA ITS1 sequence analysis based genotoxicity assessment of Dicofol by using Culex quenquefasciatus (DIPTERA: CULICIDAE). IJPBS 2015d; 6: 744-4.
- Bhinder P, Chaudhary A. Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay. Toxicology International, 2014; 21 (1): 84-8.
- Gaulden M E. Hypothesis: some mutagens directly alter specific chromosomal protein (DNA topoisomerase II and peripheral protein) to produce chromosome stickiness which cause chromosome aberrations. Mutagenesis 1987; 2: 357-5.
- Purdy A, Su TT 2004. Telomeres: Not all breaks are equal. Curr Biol, 2004; 14: 613-9.
- Gilley D, Tanaka H, Herbert B S. Telomere dysfunction in aging and cancer. Int J Biochem Cell Biol 2005; 37: 1000-3.
- Michailova P V. Rearrangements in Chironomidae (Diptera) genomes induced by various environmental stress factor. Becmhunk. Botuc 2010; 14: 43-3.
- Michailova P, Belcheva P. Different effects of lead on external morphology and polytene chromosomes of Glyptotendipes barbipes (Staeger), (Diptera: Chironomidae). Folia Biol 1990; 38: 83-8.
- Michailova P, Todorova K, White K. The effect of lead on the salivary gland chromosomes of Glyptotendipes salinus Michailova (Chironomidae, Diptera). Biologia 2002; 57(Pt3): 359-7.
- Henderson D S, Glover D M. Chromosome fragmentation resulting from an inability to repair transposase-induced DNA double-strand breaks in PCNA mutants of Drosophila. Mutagenesis 1998; 13 (Ptl): 57-6.
- Chen S, McElroy J S, Flessnera M L, Dane F. Utilizing next-generation sequencing to study homeologous polymorphisms and herbicide-resistance-endowing mutations in Poa annua acetolactate synthase genes. Pest Manag Sci 2014; 71(Pt8):1141-8.
- Gawarammana I B, Roberts D M, Mohamed F. Acute human self-poisoning with bispyribac- containing herbicide Nomineel- A prospective observational study. Clin Toxicol (Phila) 2010; 48(3); 198-2.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 563
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 2