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Microbial Production and Purification of Cellulase Enzyme on Waste as a Substrate
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The cellulase is of industrial importance and there is a high demand to produce low cost cellulase. The cellulase production was carried out by Aspergillus niger on wastes like saw dust and wheat straw, as it is a need to reduce and reuse waste from the environment. The production was optimized at 72 hrs for saw dust and wheat straw with enzyme activity of 249.10 U/ ml/min and 338.02 U/ml/min respectively. The produced enzyme was purified, the specific enzyme activity of Cellulase after precipitation at 80% (NH4)2SO4 was observed as 4.71 U/min/mg for saw dust and 6.91 U/min/mg for wheat straw respectively. After ion-exchange chromatography best specific enzyme activity was observed in saw dust at 0.6 M NaCl with 3498.04 U/min/mg and for wheat straw at 0.4 M NaCl with 1692.6 U/min/mg. The industrial importance is to produce cellulase at low cost which can be achieved using waste as substrate as well as reduces toxicity of waste.
Keywords
Aspergillus niger, Cellulase, Fermentation, Purification.
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