Toxicology International (Formerly Indian Journal of Toxicology)

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An Acute Toxicity Study of Tylophora Indica Leaves Extract in Wistar Rats


Affiliations
1 Department of Veterinary Pathology, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
2 Department of Veterinary Pathology, Maharashtra Animal and Fishery Sciences University, MAFSU, Nagpur – 440006, Maharashtra, India
3 Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab – 144411, India
4 Department of Veterinary Public Health, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
5 Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
6 Hospital Registrar, TVCC Complex, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
     

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The present investigation was carried out to evaluate the safety of hydroalcoholic extract of Tylophora indica leaves after single dose administration to determine its potential toxicity individually as per Organisation for Economic Co-operation and Development [OECD] 423 guideline. General behaviour, adverse effects and mortality parameters were studied for up to 14 days. Extract was found safe at the oral doses of 2000 mg/kg b. wt. There were no apparent toxicity signs and death observed during experimental trial. Gross necropsy examination revealed no abnormal lesions. In view of the dose of Tylophora indica leaves consumed in traditional medicine, it is observed that there is a wide margin of safety for the therapeutic use.

Keywords

Acute Toxicity, OECD Guideline 423, Tylophora Indica, Wistar Rats.
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  • Meyer SA, Kulkarni AP. Hepatotoxicity. Introduction to Biochemical Toxicology. 3rd edn. New York: John Wiley and Sons; 2001. p. 487.

  • Wolf PL. Biochemical diagnosis of liver diseases. Indian J Clin Biochem. 1999; 14:59–90. PMid: 23105203 PMCid: PMC3453559. https://doi.org/10.1007/BF02869152.

  • Rao GMM, Rao CV, Pushpangadan P, Shirwaikar A. Hepatoprotective effects of rubiadin, a major constituent of Rubia cordifolia Linn. Journal of Ethnopharmacology. 2006; 103(3):484–90. PMid: 16213120. https://doi.org/10.1016/j.jep.2005.08.073.

  • Thomas S, Seethe Devi B. Phytochemical and in vitro anthelmintic studies of hydro-alcoholic extract of Costus pictus D. Don. Int J of Pharm Sci. 2013; 5(3):639–41.

  • Ayurvedic Pharmacopeia of India. Part I, Vol VI. Appendix6; 2008. p. 247.

  • OECD/OCDE OECD Guideline for testing of chemicals. Acute Oral Toxicity - Acute Toxicity Class Method; 2011. p. 423.

  • Raina V, Raina S. The responsiveness of leukocyte adenyl cyclase to tylophorine in asthmatic subjects. Biochemical and Biophysical Research Communications. 1980; 94(4):1074– 7. https://doi.org/10.1016/0006-291X(80)90528-8.

  • Mujeeb M, Aeri V, Bagri P, Khan SA. Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill leaves IJGP. 2009; 3(2):125–7. https://doi.org/10.4103/0973-8258.54901.


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  • An Acute Toxicity Study of Tylophora Indica Leaves Extract in Wistar Rats

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Authors

N. S. Bembde
Department of Veterinary Pathology, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
P. V. Meshram
Department of Veterinary Pathology, Maharashtra Animal and Fishery Sciences University, MAFSU, Nagpur – 440006, Maharashtra, India
P. D. Gadhave
Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab – 144411, India
V. M. Vaidya
Department of Veterinary Public Health, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
S. S. Sole
Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India
R. R. Rohi
Hospital Registrar, TVCC Complex, Mumbai Veterinary College, Mumbai – 400012, Maharashtra, India

Abstract


The present investigation was carried out to evaluate the safety of hydroalcoholic extract of Tylophora indica leaves after single dose administration to determine its potential toxicity individually as per Organisation for Economic Co-operation and Development [OECD] 423 guideline. General behaviour, adverse effects and mortality parameters were studied for up to 14 days. Extract was found safe at the oral doses of 2000 mg/kg b. wt. There were no apparent toxicity signs and death observed during experimental trial. Gross necropsy examination revealed no abnormal lesions. In view of the dose of Tylophora indica leaves consumed in traditional medicine, it is observed that there is a wide margin of safety for the therapeutic use.

Keywords


Acute Toxicity, OECD Guideline 423, Tylophora Indica, Wistar Rats.

References





DOI: https://doi.org/10.18311/ti%2F2018%2Fv25i3%2F23751