Toxicology International (Formerly Indian Journal of Toxicology)

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Toxicological Investigation of Single Oral Dose Administration of Imidacloprid in Male Wistar Rats


Affiliations
1 College of Veterinary Sciences, GADVASU, Ludhiana – 141004, Punjab, India
2 Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly– 243122, Uttar Pradesh, India
     

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The aim of this study was to evaluate the acute toxic effect of Imidacloprid (IM) on adult wistar rats. Rats were divided into four different groups. Animals from Group I and II were received water and corn oil, respectively, while, Group III and IV were received oral dose of IM @ 150mg/kg and 300mg/kg body weight, respectively. Thereafter, animals were subjected to series of neuro-toxicity related parameters (general behavioral changes, motor activity, pentobarbitone induced sleeping time and measurement of pain response through mechanical stimulation, radiant heat and tail clip method). IM administration in rats produced significant behavioral depression which resulted into decrease in alertness, awareness and grip strength, and there was reduction in the response to sound, touch and pinna reflexes as compared control. Further, IM resulted in significant decrease in spontaneous locomotor activity and forced locomotor activity (rota-rod performance). IM administration significantly increased the pentobarbitone-induced sleeping time without altering the onset of sleeping time. Orally administered IM did not produce any significant analgesic or hyperalgesic effect. The present study suggests that IM has pronounced CNS effect without effecting analgesic activity during acute toxicosis in animals.


Keywords

Analgesic Activity, Imidacloprid, Motor Activity, Sleeping Time.
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  • Toxicological Investigation of Single Oral Dose Administration of Imidacloprid in Male Wistar Rats

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Authors

M. K. Lonare
College of Veterinary Sciences, GADVASU, Ludhiana – 141004, Punjab, India
Manoj Kumar
Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly– 243122, Uttar Pradesh, India
A. More
Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly– 243122, Uttar Pradesh, India
A. G. Telang
Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly– 243122, Uttar Pradesh, India

Abstract


The aim of this study was to evaluate the acute toxic effect of Imidacloprid (IM) on adult wistar rats. Rats were divided into four different groups. Animals from Group I and II were received water and corn oil, respectively, while, Group III and IV were received oral dose of IM @ 150mg/kg and 300mg/kg body weight, respectively. Thereafter, animals were subjected to series of neuro-toxicity related parameters (general behavioral changes, motor activity, pentobarbitone induced sleeping time and measurement of pain response through mechanical stimulation, radiant heat and tail clip method). IM administration in rats produced significant behavioral depression which resulted into decrease in alertness, awareness and grip strength, and there was reduction in the response to sound, touch and pinna reflexes as compared control. Further, IM resulted in significant decrease in spontaneous locomotor activity and forced locomotor activity (rota-rod performance). IM administration significantly increased the pentobarbitone-induced sleeping time without altering the onset of sleeping time. Orally administered IM did not produce any significant analgesic or hyperalgesic effect. The present study suggests that IM has pronounced CNS effect without effecting analgesic activity during acute toxicosis in animals.


Keywords


Analgesic Activity, Imidacloprid, Motor Activity, Sleeping Time.

References





DOI: https://doi.org/10.18311/ti%2F2019%2Fv26i1%2F22966